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Normal Sera


Non-immune sera from goat, human, mouse, rabbit and other species.

Pierce Normal Serum

Thermo Scientific Pierce Normal Sera are non-immune serum samples from goat, human, mouse, rabbit and other species that are popular blocking agents for immunohistochemical (IHC) staining and cellular imaging methods.

Normal serum that is derived from the same or related species of animal as a tissue sample or from the host species of specific antibodies used in immunodetection method provides several properties that improve assay performance for immunohistochemistry. Normal serum provides a sufficient quantity of endogenous proteins to saturate and block nonspecific binding sites on fixed samples or assay substrates. When used as an antibody diluent, non-immune serum also supplies a native chemical environment for antibodies to function properly without adding foreign protein components.

Highlights:

  • Multiple species available – choose normal serum from goat, horse, human, mouse, rabbit, rat and swine
  • Trouble-free formulation – delipidated (lipid-extracted) and dialyzed against weak phosphate-buffered saline (PBS) containing sodium azide
  • Stable format – supplied lyophilized (from PBS with preservative) for long shelf life and easy reconstitution in water
  • Safe source – USA is country of origin for all these animal-derived products

Product Details:

Normal serum is the term used to describe serum from an animal that has not been immunized to produce antibodies against specific antigens. Thus, normal serum is the opposite of anti-serum. Normal sera contain the usual complement of serum proteins, including the various classes of immunoglobulins that exist in healthy animals of the particular species. Normal serum is frequently used for blocking or saturating generalized binding interactions for immunodetection methods, especially those involving tissue samples such as immunohistochemistry (IHC). Normal sera are also useful as controls for testing general and specific antibody purification methods.

References:

  1. Alegria-Schaffer, A., et al. (2009). Performing and optimizing Western blots with an emphasis on chemiluminescent detection. Methods Enzymol. 463:573-99.

Related Resources:

Blocking buffers for Western blot and ELISA -- technical guide
Blocking strategies for immunohistochemistry -- technical guide

Related Products:

All Blocking Buffers
Nonspecific Antibodies (purified immunoglobulins and gamma globulins)


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
31872 Normal Goat Serum
Formulation: Lyophilized powder from delipidated and PBS-dialyzed serum
2mL MSDS for product #31872 Normal Goat Serum Certificate of Analysis for product #31872 Normal Goat Serum $35.00

Add to cart
31873 Normal Goat Serum
Formulation: Lyophilized powder from delipidated and PBS-dialyzed serum
10mL MSDS for product #31873 Normal Goat Serum Certificate of Analysis for product #31873 Normal Goat Serum $99.00

Add to cart
31874 Normal Horse Serum
Formulation: Lyophilized powder from delipidated and PBS-dialyzed serum
2mL MSDS for product #31874 Normal Horse Serum Certificate of Analysis for product #31874 Normal Horse Serum $35.00

Add to cart
31876 Normal Human Serum
Formulation: Lyophilized powder from delipidated and PBS-dialyzed serum
2mL MSDS for product #31876 Normal Human Serum Certificate of Analysis for product #31876 Normal Human Serum $35.00

Add to cart
31880 Normal Mouse Serum
Formulation: Lyophilized powder from delipidated and PBS-dialyzed serum
2mL MSDS for product #31880 Normal Mouse Serum Certificate of Analysis for product #31880 Normal Mouse Serum $45.00

Add to cart
31881 Normal Mouse Serum
Formulation: Lyophilized powder from delipidated and PBS-dialyzed serum
5mL MSDS for product #31881 Normal Mouse Serum Certificate of Analysis for product #31881 Normal Mouse Serum $65.00

Add to cart
31883 Normal Rabbit Serum
Formulation: Lyophilized powder from delipidated and PBS-dialyzed serum
5mL MSDS for product #31883 Normal Rabbit Serum Certificate of Analysis for product #31883 Normal Rabbit Serum $65.00

Add to cart
31884 Normal Rabbit Serum
Formulation: Lyophilized powder from delipidated and PBS-dialyzed serum
2mL MSDS for product #31884 Normal Rabbit Serum Certificate of Analysis for product #31884 Normal Rabbit Serum $35.00

Add to cart
31888 Normal Rat Serum
Formulation: Lyophilized powder from delipidated and PBS-dialyzed serum
2mL MSDS for product #31888 Normal Rat Serum Certificate of Analysis for product #31888 Normal Rat Serum $35.00

Add to cart
31890 Normal Swine Serum
Formulation: Lyophilized powder from delipidated and PBS-dialyzed serum
2mL MSDS for product #31890 Normal Swine Serum Certificate of Analysis for product #31890 Normal Swine Serum $35.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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