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Pierce High Capacity Endotoxin Removal Resin


A quick method to remove endotoxins (lipopolysaccharides or LPS) from samples.

Pierce High Capacity Endotoxin Removal Spin Columns

Thermo Scientific Pierce High Capacity Endotoxin Removal Resin binds to reduce endotoxin levels in protein samples by ≥99% in 1 hour while maximizing protein recovery.

The Pierce High Capacity Endotoxin Removal Resin combines porous cellulose beads and an FDA-approved food preservative, poly(ε-lysine), as an affinity ligand to selectively bind endotoxins. Endotoxin levels in protein samples are reduced by ≥99% in as little as 1 hour using our spin cup format, and protein recovery is ≥85%. The modified polylysine affinity ligand eliminates the toxicity concerns associated with alternative technologies using polymixin B ligands and sodium deoxycholate buffers. Pierce High Capacity Endotoxin Removal Resin is available as a slurry to pack a custom column that can be re-used up to 10 times with no loss in endotoxin removal efficiency. This resin is also available in a new single-use spin column format optimized for different sample volumes.

Highlights:

  • High capacity – bind up to 2,000,000 EU/mL to eliminate >99% of endotoxins
  • Durable – re-use resin up to 10 times with no loss in performance
  • Selective – recover ≥85% of your protein sample
  • High performance – meets FDA guidelines by reducing final EU concentration to <5 EU/mL
  • Fast – our new spin cup format enables endotoxin depletion within 1 hour
  • Clean – single-use spin columns avoid cross contamination of samples
  • Optimized – spin cups are optimized for different sample volumes
  • Economical – large-volume discounts available

Requires:

Endotoxin-free water

Applications:

Eliminate endotoxins from protein or antibody samples prior to cell transfection or animal injection

Product Details:

The presence of endotoxins in biologically derived products prepared for research, vaccination, or therapeutic use is a major concern due to the diverse and potentially harmful biological response to these molecules. Endotoxin contamination in proteins purified from bacterial cultures is due to residual lipopolysaccharides (LPS) derived from the outer cell membrane of gram-negative bacteria such as E. coli. Endotoxin-contaminated protein samples transfected into cells or injected into an animal host can initiate a strong immune response resulting in a Systemic Inflammatory Response Syndrome (SIRS) and/or sepsis. Traditional endotoxin removal resins that use polymixin B and sodium deoxycholate to clear endotoxins from a sample also have the negative side effects of neurotoxicity and acute renal tubular necrosis.

Pierce High Capacity Endotoxin Removal Resin

Pierce High Capacity Endotoxin Removal Resin is a porous cellulose bead that has been surface-modified with covalently attached polylysine chains that have a high affinity for LPS but lacks the toxicity of other endotoxin-binding molecules. With a 2,000,000-EU/mL binding capacity, endotoxin levels can be reduced by 99% in samples containing initial endotoxin levels of 10,000 EU/mL. Typical protein samples processed with the High Capacity Endotoxin Removal Resin have a final endotoxin concentration below 5 EU/mL. This resin is offered in a slurry format for custom packing of endotoxin removal columns for gravity flow or flow rates ranging from 10-15mL/hour. Alternatively, the spin cup format is a fast, single-use method to remove endotoxins from protein samples in as little as 1 hour. The spin cups use a batch format to bind and remove endotoxins while allowing for >85% protein recovery and are available in 3 sizes to process protein samples of different volumes.

Pierce High Capacity Endotoxin Removal Resin

Pierce High Capacity Endotoxin Removal Resin protocol summary.
1.
Centrifuge at 500Xg for 1 minute to remove the storage buffer.
2. Regenerate with either 0.2N NaOH (overnight) or 0.2N NaOH in 95% ethanol for 1-2 hours.
3. Wash with 2M NaCl followed by ET-free water.
4. Equilibrate with ET-free buffer, pH 6-8. Repeat three times.
5. Add sample and incubate at 4-22°C with gentle end-over-end mixing for 1 hour.
6. Centrifuge at 500 X g for 1 minute to collect the sample.


Thermo Scientific Pierce High Capacity Endotoxin Removal Resin eliminates >99% of endotoxins from various protein samples.
Protein
(1mg/mL)
Molecular weight pI Endotoxin removal Protein recovery
Initial
(EU/mL)
Final
(EU/mL)
Efficiency
Cytochrome C 12,000 10.6 10,000 1.35 >99.9% 86%
Myoglobin 17,000 6.8 10,000 3.67 >99.9% 87%
Bovine serum albumin (BSA) 66,000 4.9 10,000 0.80 >99.9% 85%
Bovine gamma globulin (BGG) 150,000 7.4 10,000 4.60 >99.9% 92%
The 0.5-mL Pierce High Capacity Endotoxin Removal Spin column was challenged with 1mL of a protein solution (1mg containing 10,000 EU E.Coli strain 055:B5 in 50mM sodium phosphate, 0.15M NaCl and 0.05% sodium azide, pH 7.2) and incubated with gentle end-over-end mixing at room temperature (22°C) for 1 hour. Samples were collected by centrifugation at 500 x g for one minute. Protein recovery was determined using the BCA Protein Assay and the endotoxin concentration was determined using the Pierce LAL Chromogenic Endotoxin Quantitation Kit.

More Product Data

One endotoxin unit/mL (EU/mL) equals approximately 0.1ng endotoxin/mL of solution.

Related Products:

Detoxi-Gel Endotoxin Removal Gel (a more economical alternative for endotoxin removal)
Pierce LAL Chromogenic Endotoxin Quantitation Kit
Fusion Protein Purification Resins and Kits


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
88270 Pierce High Capacity Endotoxin Removal Resin
Formulation: Proprietary ligand on beaded cellulose slurried in 20% ethanol
Sufficient For: Removing (per mL of resin) >99.9% of 10,000 endotoxin units from 5mL test samples
10mL Product Instructions for product #88270 Pierce High Capacity Endotoxin Removal Resin MSDS for product #88270 Pierce High Capacity Endotoxin Removal Resin Certificate of Analysis for product #88270 Pierce High Capacity Endotoxin Removal Resin $265.00

Add to cart
88271 Pierce High Capacity Endotoxin Removal Resin
Formulation: Proprietary ligand on beaded cellulose slurried in 20% ethanol
Sufficient For: Removing (per mL of resin) >99.9% of 10,000 endotoxin units from 5mL test samples
100mL Product Instructions for product #88271 Pierce High Capacity Endotoxin Removal Resin MSDS for product #88271 Pierce High Capacity Endotoxin Removal Resin Certificate of Analysis for product #88271 Pierce High Capacity Endotoxin Removal Resin $2,101.00

Add to cart
88272 Pierce High Capacity Endotoxin Removal Resin
Formulation: Proprietary ligand on beaded cellulose slurried in 20% ethanol
Sufficient For: Removing (per mL of resin) >99.9% of 10,000 endotoxin units from 5mL test samples
250mL Product Instructions for product #88272 Pierce High Capacity Endotoxin Removal Resin MSDS for product #88272 Pierce High Capacity Endotoxin Removal Resin Certificate of Analysis for product #88272 Pierce High Capacity Endotoxin Removal Resin $4,500.00

Add to cart
88272B Pierce High Capacity Endotoxin Removal Resin
Formulation: Proprietary ligand on beaded cellulose slurried in 20% ethanol
Sufficient For: Removing (per mL of resin) >99.9% of 10,000 endotoxin units from 5mL test samples
Custom
88273 Pierce High Capacity Endotoxin Removal Spin Columns, 0.25mL
Formulation: 5mL-capacity centrifuge columns containing 0.25mL resin
Sufficient For: Removing >99.9% of the endotoxin present in typical 0.5 to 1mL samples
5 columns Product Instructions for product #88273 Pierce High Capacity Endotoxin Removal Spin Columns, 0.25mL MSDS for product #88273 Pierce High Capacity Endotoxin Removal Spin Columns, 0.25mL Certificate of Analysis for product #88273 Pierce High Capacity Endotoxin Removal Spin Columns, 0.25mL $49.00

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88274 Pierce High Capacity Endotoxin Removal Spin Columns, 0.5mL
Formulation: 8mL-capacity centrifuge columns containing 0.5mL resin
Sufficient For: Removing >99.9% of the endotoxin present in typical 1 to 4mL samples
5 columns Product Instructions for product #88274 Pierce High Capacity Endotoxin Removal Spin Columns, 0.5mL MSDS for product #88274 Pierce High Capacity Endotoxin Removal Spin Columns, 0.5mL Certificate of Analysis for product #88274 Pierce High Capacity Endotoxin Removal Spin Columns, 0.5mL $57.00

Add to cart
88275 Pierce High Capacity Endotoxin Removal Spin Columns, 0.5mL
Formulation: 8mL-capacity centrifuge columns containing 0.5mL resin
Sufficient For: Removing >99.9% of the endotoxin present in typical 1 to 4mL samples
25 columns Product Instructions for product #88275 Pierce High Capacity Endotoxin Removal Spin Columns, 0.5mL MSDS for product #88275 Pierce High Capacity Endotoxin Removal Spin Columns, 0.5mL Certificate of Analysis for product #88275 Pierce High Capacity Endotoxin Removal Spin Columns, 0.5mL $205.00

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88276 Pierce High Capacity Endotoxin Removal Spin Columns, 1mL
Formulation: 22mL-capacity centrifuge columns containing 1mL resin
Sufficient For: Removing >99.9% of the endotoxin present in typical 2 to 10mL samples
5 columns Product Instructions for product #88276 Pierce High Capacity Endotoxin Removal Spin Columns, 1mL MSDS for product #88276 Pierce High Capacity Endotoxin Removal Spin Columns, 1mL Certificate of Analysis for product #88276 Pierce High Capacity Endotoxin Removal Spin Columns, 1mL $159.00

Add to cart
88277 Pierce High Capacity Endotoxin Removal Spin Columns, 1mL
Formulation: 22mL-capacity centrifuge columns containing 1mL resin
Sufficient For: Removing >99.9% of the endotoxin present in typical 2 to 10mL samples
25 columns Product Instructions for product #88277 Pierce High Capacity Endotoxin Removal Spin Columns, 1mL MSDS for product #88277 Pierce High Capacity Endotoxin Removal Spin Columns, 1mL Certificate of Analysis for product #88277 Pierce High Capacity Endotoxin Removal Spin Columns, 1mL $523.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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