Thermo Scientific Pierce High Capacity Endotoxin Removal Resin binds to reduce endotoxin levels in protein samples by ≥99% in 1 hour while maximizing protein recovery.
The Pierce High Capacity Endotoxin Removal Resin combines porous cellulose beads and an FDA-approved food preservative, poly(ε-lysine), as an affinity ligand to selectively bind endotoxins. Endotoxin levels in protein samples are reduced by ≥99% in as little as 1 hour using our spin cup format, and protein recovery is ≥85%. The modified polylysine affinity ligand eliminates the toxicity concerns associated with alternative technologies using polymixin B ligands and sodium deoxycholate buffers. Pierce High Capacity Endotoxin Removal Resin is available as a slurry to pack a custom column that can be re-used up to 10 times with no loss in endotoxin removal efficiency. This resin is also available in a new single-use spin column format optimized for different sample volumes.
- High capacity – bind up to 2,000,000 EU/mL† to eliminate >99% of endotoxins
- Durable – re-use resin up to 10 times with no loss in performance
- Selective – recover ≥85% of your protein sample
- High performance – meets FDA guidelines by reducing final EU concentration to <5 EU/mL
- Fast – our new spin cup format enables endotoxin depletion within 1 hour
- Clean – single-use spin columns avoid cross contamination of samples
- Optimized – spin cups are optimized for different sample volumes
- Economical – large-volume discounts available
Eliminate endotoxins from protein or antibody samples prior to cell transfection or animal injection
The presence of endotoxins in biologically derived products prepared for research, vaccination, or therapeutic use is a major concern due to the diverse and potentially harmful biological response to these molecules. Endotoxin contamination in proteins purified from bacterial cultures is due to residual lipopolysaccharides (LPS) derived from the outer cell membrane of gram-negative bacteria such as E. coli. Endotoxin-contaminated protein samples transfected into cells or injected into an animal host can initiate a strong immune response resulting in a Systemic Inflammatory Response Syndrome (SIRS) and/or sepsis. Traditional endotoxin removal resins that use polymixin B and sodium deoxycholate to clear endotoxins from a sample also have the negative side effects of neurotoxicity and acute renal tubular necrosis.
Pierce High Capacity Endotoxin Removal Resin is a porous cellulose bead that has been surface-modified with covalently attached polylysine chains that have a high affinity for LPS but lacks the toxicity of other endotoxin-binding molecules. With a 2,000,000-EU/mL binding capacity, endotoxin levels can be reduced by 99% in samples containing initial endotoxin levels of 10,000 EU/mL. Typical protein samples processed with the High Capacity Endotoxin Removal Resin have a final endotoxin concentration below 5 EU/mL. This resin is offered in a slurry format for custom packing of endotoxin removal columns for gravity flow or flow rates ranging from 10-15mL/hour. Alternatively, the spin cup format is a fast, single-use method to remove endotoxins from protein samples in as little as 1 hour. The spin cups use a batch format to bind and remove endotoxins while allowing for >85% protein recovery and are available in 3 sizes to process protein samples of different volumes.
Pierce High Capacity Endotoxin Removal Resin protocol summary.
1. Centrifuge at 500Xg for 1 minute to remove the storage buffer.
2. Regenerate with either 0.2N NaOH (overnight) or 0.2N NaOH in 95% ethanol for 1-2 hours.
3. Wash with 2M NaCl followed by ET-free water.
4. Equilibrate with ET-free buffer, pH 6-8. Repeat three times.
5. Add sample and incubate at 4-22°C with gentle end-over-end mixing for 1 hour.
6. Centrifuge at 500 X g for 1 minute to collect the sample.
Thermo Scientific Pierce High Capacity Endotoxin Removal Resin eliminates >99% of endotoxins from various protein samples.
|Bovine serum albumin (BSA)
|Bovine gamma globulin (BGG)
|The 0.5-mL Pierce High Capacity Endotoxin Removal Spin column was challenged with 1mL of a protein solution (1mg containing 10,000 EU E.Coli strain 055:B5 in 50mM sodium phosphate, 0.15M NaCl and 0.05% sodium azide, pH 7.2) and incubated with gentle end-over-end mixing at room temperature (22°C) for 1 hour. Samples were collected by centrifugation at 500 x g for one minute. Protein recovery was determined using the BCA Protein Assay and the endotoxin concentration was determined using the Pierce LAL Chromogenic Endotoxin Quantitation Kit.
† One endotoxin unit/mL (EU/mL) equals approximately 0.1ng endotoxin/mL of solution.
Detoxi-Gel Endotoxin Removal Gel (a more economical alternative for endotoxin removal)
Pierce LAL Chromogenic Endotoxin Quantitation Kit
Fusion Protein Purification Resins and Kits