The Thermo Scientific Pierce Gaussia Luciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life greater than 1 hour), especially in the presence of Gaussia-Dura luciferase reporter.
This Pierce Luciferase Glow Assay Kit contains reagents for measuring Gaussia luciferase activity in mammalian cell culture media and lysates. When used with Thermo Scientific Gaussia-Dura Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of luciferase activity with extended signal stability. The light output generated by the luciferase reaction can be correlated to the amount of luciferase protein produced, which in turn is proportional to the activity of the promoter driving the luciferase expression. The kit can be used to detect the activity of any Gaussia luciferases or derivatives that use coelenterazine as a substrate.
- Sensitive – highly sensitive detection of Gaussia luciferase activity
- Stable – increased signal stability
- Simple – does not require a luminometer with injectors
- Compatible – assay reagents compatible with other Gaussia luciferases
- Automation-friendly – amenable to high throughput assays
- Convenient – contains a universal cell lysis buffer and optimized glow assay reagent
- Safe – allows one to perform non-radioactive assays
Cell lysis buffer, reaction buffer and substrate
Gaussia luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers.
- Promoter studies for analyzing cis- regulatory elements and trans-acting factors
- Drug screening
- siRNA and miRNA screening
- Multiplexed assays to study off-target effects
- Secretory pathway / protein localization reporter assays
- Signal transduction pathway analysis
- RNA splicing studies
Gaussia luciferase is a 20kDa protein from the marine copepod, Gaussia princeps. The bioluminescent enzyme is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. Light output generated by the luciferase reaction can be correlated to the amount of Gaussia luciferase protein produced and used to determine the activity of the promoter driving Gaussia expression. The signal produced by Gaussia luciferase shows strong flash kinetics and is considerably greater than flash signals from either firefly or Renilla luciferases assayed under similar conditions.
The Gaussia-Dura gene used in the Thermo Scientific Gaussia-Dura Luc Vectors is derived from the wild type Gaussia gene and provides equally bright but more stable light output. The Gaussia Glow Assay Kit reagent yields a Gaussia-Dura luciferase reaction with glow-type kinetics and is recommended for luminometers without injectors or for applications requiring batch processing of samples.
||Gaussia luciferase reaction. Oxidative decarboxylation of coelenterazine by Gaussia luciferase produces coelenteramide, carbon dioxide, and light.
||Thermo Scientific Pierce Gaussia Glow Assay Kit reagents results in stable bioluminescent signal. Glow assay reagents were added to sample wells containing lysates obtained from HEK293 cells transfected with Thermo Scientific pCMV-Gaussia-Dura plasmid. Total light output was captured using the luminescence module of the Thermo Scientific Varioskan Flash Multimode Reader (integration time of 1sec/reading).
- Szent-Gyorgyi, C., et al. (1999). Cloning and characterization of new bioluminescent proteins. Part of the SPIE Conference on Molecular Imaging: Reporters, Dyes, Markers, and Instrumentation. San Jose, CA. Proc. SPIE 3600:4-11.
- Tannous, B. A., et al. (2005). Codon-optimized Gaussia luciferase cDNA for mammalian gene expression in culture and in vivo. Molecular Therapy 11:435-443.
Review of Luciferase Reporters
Luciferase Reporter Assay Selection Guide
pMCS-Gaussia Dura Luc Vector and Glow Assay Kit
Gaussia Vectors, Flash and Dual Assay Kits
All Luciferase Reporter Assay Reagents and Kits
Pierce Luciferase Cell Lysis Buffer (2X)
TurboFect in vitro Transfection Reagent
Modified Dulbecco’s PBS BupH Packs
Modified Dulbecco’s PBS, 20X Solution