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Pierce Detergent Removal Spin Columns and Plates


Quickly remove detergents from protein and peptide samples before mass spectrometry.

Detergent Removal ResinThermo Scientific Pierce Detergent Removal Resin efficiently binds to and removes high concentrations of detergents from 10µL to 1mL samples with minimal sample loss and is available prepacked into spin columns and 96-well spin filter plates.

The Pierce Detergent Removal Resin specifically binds a wide variety of detergents and surfactants that are commonly used in protein extraction and biological sample preparation and provide an especially convenient and rapid format for treating protein and peptide solutions to remove interfering detergents before downstream analysis by mass spectrometry and other techniques.

Highlights:

  • Fast and efficient protocol
  • Removes greater than 95% of detergents with high protein recovery
  • Helps improves mass spectrometry peptide coverage
  • Best for processing samples with proteins or peptides at greater than 100µg/mL
    (for low-concentration samples, consider our HiPPR Detergent Removal Products)

Product Details:

Detergent removal from peptide samples is a challenge, especially for MS analysis in which even low detergent concentrations contaminate instruments and interfere with column binding, elution and ionization. Experiments demonstrate that Pierce Detergent Removal Resin effectively removes a variety of detergents from BSA and HeLa cell lysate tryptic digests, enabling improved LC-MS/MS and MALDI-MS analysis. After processing samples, the high baseline caused by detergents is reduced or eliminated. Analysis of the digested HeLa cell lysates by LC-MS/MS resulted in an approximate four-fold increase of identified peptides in the treated sample compared to the detergent-contaminated sample and equivalent numbers of peptides compared to a control sample, indicating minimal losses of peptides.

We recommend Pierce Detergent Removal Resin for samples having protein or peptide concentrations greater than 100µg/mL. Use the HiPPR Detergent Removal Resin (where "HiPPR" denotes High Protein and Peptide Recovery) for sample concentrations of 1 to 100µg/mL.

A. Spin Column Protocol

Protocol for removing detergent from protein samples with the Pierce Detergent Removal Spin Columns.
 

B. Spin Plate Protocol

Protocol summary for Thermo Scientific Pierce Detergent Removal Spin Columns.
 

Protocol summary for Thermo Scientific Pierce Detergent Removal Resin.

 

MALDI-MS analysis of a BSA tryptic digest before and after removal of Triton-X100 Detergent and Triton-X114 Detergent. Peaks corresponding to detergents are eliminated in processed samples, allowing reliable peptide/protein identification. BSA tryptic digest (0.1mL, 100µg) containing a detergent was processed through 0.5mL of Pierce Detergent Removal Resin and subjected to MALDI-MS analysis on a MALDI-Orbitrap Mass Spectrometer. Similar results were produced for samples containing CHAPS, NP-40 and SDS (data not shown).

 

Graph comparing the number of peptides identified from 1% SDS HeLa cell lysate with and without removal of SDS detergent with the Pierce Detergent Removal Columns Effective detergent removal enables greater peptide identification. A tryptic digest of HeLa cell lysate (0.1mL, 100µg) containing 1% SDS was processed through 0.5ml of Pierce Detergent Removal Resin and subjected to LC-MS/MS analysis. The processed sample allowed similar numbers of identified peptides as digests containing no SDS. Peptide identification is greatly reduced in sample containing SDS.

 

Detergent are effectively removed with high protein recovery Samples (0.1mL containing 100µg BSA and detergent) were processed through 0.5mL of Pierce Detergent Removal Resin and the percent detergent removed was determined. Similar results were produced for insulin (5.7kDa), α-lactalbumin (14.2kDa) and carbonic anhydrase (29kDa) (data not shown).
Detergent Detergent Removal (%) BSA Recovery (%)
SDS, 2.5% 99 95
Sodium deoxycholate, 5% 99 100
CHAPS, 3% 99 90
Octyl glucoside, 5% 99 90
Octyl thioglucoside, 5% 99 95
Lauryl maltoside, 1% 98 99
Triton™ X-100, 2% 99 87
Triton X-114, 2% 95 100
NP-40, 1% 95 91
Brij™-35, 1% 99 97

Related Resources:

Review of sample preparation for mass spectrometry

Related Products:

HiPPR Detergent Removal Columns
Detergents for Cell Lysis
Pierce Cell Lysis Reagents
Pierce C-18 Spin Columns

Available Formats:

  • Resin Slurries – 10mL bottle
  • Spin Columns – 0.125mL, 0.5mL (microcentrifuge), 2mL, 4mL columns
  • 96-well Spin Plates – pre-dispensed 96-well filter plates, compatible with centrifugation and vacuum manifold systems for manual or automated purification. The plates enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput separations. See Detergent Removal 96-well Spin Plate data.

Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
87780 Pierce Detergent Removal Resin
Formulation: Proprietary resin slurried in 150mM NaCl, 0.05% sodium azide
Sufficient For: 1 to 2.5mL of typical detergent-containing sample
10mL Product Instructions for product #87780 Pierce Detergent Removal Resin MSDS for product #87780 Pierce Detergent Removal Resin $269.00

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87776 Pierce Detergent Removal Spin Column, 125µL
Formulation: Microcentrifuge columns containing 125µL of proprietary resin
Sufficient For: 10 to 25µL sample with each column
25 columns Product Instructions for product #87776 Pierce Detergent Removal Spin Column, 125µL MSDS for product #87776 Pierce Detergent Removal Spin Column, 125µL $139.00

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87777 Pierce Detergent Removal Spin Column, 0.5mL
Formulation: Microcentrifuge columns containing 0.5mL of proprietary resin
Sufficient For: 25 to 100µL sample with each column
25 columns Product Instructions for product #87777 Pierce Detergent Removal Spin Column, 0.5mL MSDS for product #87777 Pierce Detergent Removal Spin Column, 0.5mL $174.00

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87778 Pierce Detergent Removal Spin Column, 2mL
Formulation: Centrifuge columns containing 2mL of proprietary resin
Sufficient For: 150 to 500µL sample with each column
5 columns Product Instructions for product #87778 Pierce Detergent Removal Spin Column, 2mL MSDS for product #87778 Pierce Detergent Removal Spin Column, 2mL $92.00

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87779 Pierce Detergent Removal Spin Column, 4mL
Formulation: Centrifuge columns containing 4mL of proprietary resin
Sufficient For: 500 to 1000µL sample with each column
5 columns Product Instructions for product #87779 Pierce Detergent Removal Spin Column, 4mL MSDS for product #87779 Pierce Detergent Removal Spin Column, 4mL $126.00

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88304 Pierce Detergent Removal Spin Plates
Formulation: 96-well filter plates containing 0.55mL resin slurry per well; Includes 2 wash plates and 2 collection plates
Sufficient For: 192 samples of 25 to 100µL each

Learn more about Pierce 96-well Filter Spin Plates.
2 plates Product Instructions for product #88304 Pierce Detergent Removal Spin Plates MSDS for product #88304 Pierce Detergent Removal Spin Plates Certificate of Analysis for product #88304 Pierce Detergent Removal Spin Plates $440.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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