The Thermo Scientific Pierce Cypridina Luciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Cypridina luciferase reporters.
This Pierce Luciferase Glow Assay Kit contains reagents for measuring Cypridina luciferase activity in mammalian cell culture media and lysates. When used with Thermo Scientific Cypridina Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of luciferase activity. The light output generated by the luciferase reaction can be correlated to the amount of luciferase protein produced, which in turn is proportional to the activity of the promoter driving the luciferase expression. The kit can be used to detect the activity of any Cypridina luciferases or derivatives that use Vargulin as a substrate.
- Sensitive – highly sensitive detection of Cypridina luciferase activity
- Secreted luciferase – allows real-time assays and kinetic studies without destroying cells
- Stable – reagents provide increased signal stability compared to flash assays
- Simple – does not require a luminometer with injectors
- Compatible – assay reagents compatible with other Cypridina or Vargula luciferases
- Automation-friendly – amenable to high throughput assays
- Convenient – contains a universal cell lysis buffer and optimized glow assay reagent
- Safe – allows one to perform non-radioactive assays
Cell lysis buffer, reaction buffer and substrate
Cypridina luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers.
- Promoter studies for analyzing cis- regulatory elements and trans-acting factors
- Drug screening
- siRNA and miRNA screening
- Multiplexed assays to study off-target effects
- Secretory pathway / protein localization reporter assays
- Signal transduction pathway analysis
- RNA splicing studies
The Cypridina luciferase is a 61kDa protein from the marine ostracod, Cypridina noctiluca. Cypridina luciferase is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. Light output captured using a luminometer can be correlated with the amount of Cypridina luciferase protein produced and used to determine the activity of the promoter driving Cypridina expression. The Cypridina gene in Thermo Scientific Cypridina Luc Vectors is modified for expression in mammalian cells and provides optimal performance with the assay kit. The Cypridina glow reagent yields a Cypridina luciferase reaction with glow-type kinetics and is recommended for luminometers without injectors or for applications requiring batch processing of samples.
||Cypridina luciferase reaction. Cypridina luciferin, also known as Vargulin, substrate is oxidized by Cypridina luciferase, producing Cypridina oxyluciferin, carbon dioxide and light.
||Thermo Scientific Pierce Cypridina Glow Assay Kit reagents results in stable bioluminescent signal. Glow assay reagent was added to sample wells containing media obtained from HEK293 cells transfected with Pierce pCMV-Cypridina plasmid. Total light output was captured using the luminescence module of the Thermo Scientific Varioskan Flash Multimode Reader (integration time of 1sec/reading). The graph demonstrates the initial flash signal from Cypridina luciferase which is followed by a glow signal that is stable over 30 minutes.
- Nakajima, Y., et al. (2004). cDNA Cloning and characterization of a secreted luciferase from the luminous Japanese ostracod, Cypridina noctiluca. Biosci. Biotechnol. Biochem. 68(3):565-570.
Review of Luciferase Reporters
Luciferase Reporter Assay Selection Guide
Cypridina Vectors, Flash and Dual Assay Kits
All Luciferase Reporter Assay Reagents and Kits
Pierce Luciferase Cell Lysis Buffer (2X)
TurboFect in vitro Transfection Reagent
Modified Dulbecco’s PBS BupH Packs
Modified Dulbecco’s PBS, 20X Solution