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Pierce 96-well Microdialysis Plates


Convenient and rapid dialysis system for 1 to 96 small samples.

Pierce 96-well Microdialysis Plates

The Thermo Scientific Pierce 96-well Microdialysis Plates are automation-compatible, microplate dialysis devices (3.5K or 10K MWCO) for rapidly processing 1 to 96 samples with volumes 10 to 100µL.

Each sample processed with the Pierce 96-well Microdialysis Dialysis Plate is dialyzed inside a single, removable dialysis device created by two regenerated cellulose membranes separated by ~2mm. The short diffusion distance and large surface area facilitates rapid dialysis with the removal of salts and small molecules often completed in 2 to 4 hours. Sample recovery is performed using standard laboratory micropipettes. Individual microdialysis devices can also be used with 2mL microcentrifuge tubes for processing single samples. The available plate seals are made of high-quality polypropylene and help protect samples from contamination and evaporation, especially during long-term storage.

Highlights:

  • High yield – greater than 90% protein recovery after dialysis
  • Rapid – dialysis completed in 2 to 4 hours
  • Easy to use – sample loading and retrieval can be done with a standard pipette
  • Flexible – detachable 8-device strips, scalable from 1 to 96 samples
  • Automation-compatible – plate format conforms to the SBS Microplate Standard
Video of the Pierce 96-well Microdialysis Plate in action!

Applications:

Product Details:

Each 96-well Microdialysis Plate comes pre-loaded with twelve 8-dialysis device strips. Each dialysis device strip can be removed from the plate and individual microdialysis devices easily separated, allowing only the number of needed devices to be used and eliminating waste. The sample chamber inside each microdialysis device is formed by two low-binding regenerated cellulose membranes that are rated to retain proteins and other macromolecules >10,000 Daltons while allowing removal of buffer salts and contaminants <1000 Daltons. Surface tension and the small distance between the membranes facilitate highly efficient sample recovery using standard laboratory pipettes.

Pierce Microdialyzer Plate Protocol Overview
Protocol summary for Thermo Scientific 96-well Microdialysis Plate.

Using the Microdialysis Plate, sample dialysis can be efficiently performed in a standard 96 deep-well plate with a minimal amount of buffer while still providing >95% removal of small molecules. The assembled device is compatible with standard 96-well laboratory equipment and automated liquid-handling systems making it an ideal option for high-throughput applications. Single microdialysis devices can be used independently in 2mL microcentrifuge tube for processing single samples.

Pierce Microdialyzer Plate Protocol Overview Rate of removal of NaCl in the Thermo Scientific Pierce 96-well Microdialysis Device, 10K MWCO. Samples of 0.1mL (0.4mg/mL cytochrome c containing 1M NaCl) were dialyzed against 1.8mL of water in a 96 deep-well plate at RT with gentle shaking on a plate shaker. The buffer was changed at 1-, 2- and 3-hour intervals over a 4-hour period. The rate of NaCl removal was determined by measuring the conductivity of the retentate at the indicated time intervals. Removal of NaCl was >83% after 2 hours and >99% after 4 hours.

 

Pierce 96-well Microdialysis Plate membrane chemical compatibility list. Compatibility of various solutions, rated as good (G), limited (L) or not compatible (N).
Acetic acid, 96% G     Formic acid, 25% G     Phosphoric acid, 85% N    
Acetonitrile G Formic acid, 100% N Isopropanol G
Acetone L Glycerol G 1-Propanol G
Ammonium hydroxide (1N) L Hydrochloric acid, 10% L Potassium hydroxide (1N) L
Ammonium hydroxide, 25% G Hydrochloric acid, 25% N Potassium hydroxide, 32% N
Chloroform G Hydrofluoric acid, 50% N Sodium hydroxide (1N) L
Dimethyl sulfoxide G Methanol, 98% G Sodium hydroxide, 32% N
Ethanol, 98% G Methylene chloride G Sulfuric acid, 98% N
Ethylacetate G Nitric acid, 25% N Tetrahydrofuran G
Ethylene glycol G Phosphoric acid, 25% L Toluene G
G = Good chemical resistance
L = Limited chemical resistance (poor size not guaranteed)
N = No chemical resistance, not recommended

Related Resources:

Dialysis Methods (Technical Guide)
Dialysis Products Selection Guide

Related Products:

Slide-A-Lyzer MINI Dialysis Devices (volumes 10 to 2000µL)
Rapid Equilibrium Dialysis Devices (volumes 100 to 500µL)
Slide-A-Lyzer Dialysis Concentrating Solution
Dialysis Tubing, Cassettes and Systems
Desalting Columns
Pierce Protein Concentrators
Buffers for Protein Methods


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
88262 Pierce 96-well Microdialysis Plate, 3.5K MWCO
Formulation: Dialysis membrane framed in plastic insert with 96-well deep-well plate
Sufficient For: Dialysis of 96 samples 10 to 100µL each

Kit Contents:
8-microdialysis device strip, 12 strips
96-deep well plate, 1 plate
1-plate set Product Instructions for product #88262 Pierce 96-well Microdialysis Plate, 3.5K MWCO Certificate of Analysis for product #88262 Pierce 96-well Microdialysis Plate, 3.5K MWCO $417.00

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88260 Pierce 96-well Microdialysis Plate, 10K MWCO
Formulation: Dialysis membrane framed in plastic insert with 96-well deep-well plate
Sufficient For: dialysis of 96 samples 10 to 100µL each

Kit Contents:
8-microdialysis device strip, 12 strips
96-well deep-well plate, 1 plate
1-plate set Product Instructions for product #88260 Pierce 96-well Microdialysis Plate, 10K MWCO MSDS for product #88260 Pierce 96-well Microdialysis Plate, 10K MWCO Certificate of Analysis for product #88260 Pierce 96-well Microdialysis Plate, 10K MWCO $417.00

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88261 96-well Deep-well Plate, 2.2mL
Formulation: Polypropylene deep-well assay plate
Sufficient For: 96 microdialysis devices
1 plate $15.00

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88269 Plate Seal for Pierce 96-well Microdialysis Plates
Formulation: Adhesive coated plastic sheet, perforated to 8-well strips fitting microdialysis plate
Sufficient For: 12 x 8 microdialysis plate wells
1 sheet $10.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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