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Phosphoprotein Enrichment Kit


Enrich phosphoproteins for analysis in Western blotting or mass spectrometry.

Pierce Phosphoprotein Enrichment Kit

The Thermo Scientific Pierce Phosphoprotein Enrichment Kit is designed for the efficient enrichment of phosphorylated proteins derived from mammalian cells and tissues.

The proprietary resin and buffer composition produces superior yields with negligible non-specific binding. The kit is uses a convenient spin column format and is compatible with downstream applications including Western blotting, Mass Spec, 2D-PAGE, and protein arrays. The affinity resin is also available as pre-packed chromatography cartridges for use with automated liquid chromatography (LC) systems.

Highlights:

  • Specific - low nonspecific protein contamination from complex biological samples, such as cell culture lysate and mouse tissue extract
  • Fast - easy spin format enables enrichment of phosphorylated proteins in less than 2 hours
  • Superior yield - acheives higher yields than other commercially available kits
  • Convenient format - complete kit offering pre-dispensed spin columns, buffers, reagents and ultrafiltration columns for enrichment
  • Compatible - downstream applications include mass spectrometry, Western blotting, and 2D-PAGE

Product Details:

Phosphorylation is one of the most frequently occurring post-translational modifications in proteins and it regulates nearly all intracellular biological events including, signal transduction, protein-protein interaction, protein stability, protein localization, apoptosis, and cell cycle control. Detecting changes in protein phosphorylation can be a difficult task, because of the transient labile state of the phosphate group. Furthermore, low phosphoprotein abundance and poorly developed phospho-specific antibodies also contribute to difficulties in phosphoprotein detection. Recent advances in mass spectrometry (MS) technology in combination with phosphoprotein enrichment using immobilized metal affinity chromatography (IMAC) have resulted in greater resolution of the phosphoproteome.

Faster and higher phosphoprotein yields with the Thermo Scientific Pierce Kit.
Kit Phosphoprotein
Yield (µg)
Enrichment Time
(Hours)
Thermo Scientific Pierce
Phosphoprotein Enrichment Kit
300 1.5
Supplier Q Kit 88 4.5
Supplier I Kit 52†† 3.5
Supplier C Kit 160 3
Supplier E Kit Too dilute to determine 5
From 2mg total protein; †† Based on maximum 1mg load per manufacturer’s protocol

More Product Data

Product Reference:

  1. Nilsson, C.L. et al. (2010). Quantitative phosphoproteomic analysis of the STAT3/IL-6/HIF1alpha signaling network: an initial study in GSC11 glioblastoma stem cells. J. Proteome Res. 9:430-43.

Related Resources:

Phosphoprotein Enrichment Kit FAQ
Overview of post-translational modification (PTM)
Review of sample preparation for mass spectrometry

Related Products:

Glycoprotein Isolation Kits
Ubiquitin Enrichment Kit
Halt Phosphatase Inhibitor Cocktail
Halt Protease Inhibitor Single-Use Cocktail
Protein Stabilizing Cocktail


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
90003 Pierce Phosphoprotein Enrichment Kit
Formulation: Multi-component kit
Sufficient For: Purifying 10 protein samples each containing up to 4mg total protein (400µg phosphoprotein)

Kit Contents:
Phosphoprotein Enrichment Columns, 1mL, 10 columns
Lysis/Binding/Wash Buffer, 325mL
Elution Buffer, 60mL
CHAPS Detergent, 1g
Protein Concentrators, 9K MWCO, 7mL, 10 concentrators
Column Accessories
10-column kit Product Instructions for product #90003 Pierce Phosphoprotein Enrichment Kit MSDS for product #90003 Pierce Phosphoprotein Enrichment Kit Certificate of Analysis for product #90003 Pierce Phosphoprotein Enrichment Kit $501.00

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87744 Pierce Phosphoprotein Enrichment Chromatography Cartridges, 5mL
Formulation: 5mL agarose resin cartridge with LC and Luer-Lok Fittings
Sufficient For: Purifying samples containing 20mg total protein (2mg phosphoprotein) per use

Learn more about Pierce Chromatography Cartridges.
1 cartridge Product Instructions for product #87744 Pierce Phosphoprotein Enrichment Chromatography Cartridges, 5mL MSDS for product #87744 Pierce Phosphoprotein Enrichment Chromatography Cartridges, 5mL $205.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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