Update profile
    Change password
    Sign out

Phospho p38 ELISA Kit


Microplate sandwich ELISA kit for phospho-p38 (pp38).

Thermo Scientific Phospho p38 ELISA Kits

Assay phosphorylated p38 ([pThr180/Tyr182] p38 MAP kinase, pp38) levels in cell lysates of human and mouse origin with our Thermo Scientific Phospho p38 ELISA Kit.

This phospho-p38 ELISA kit is an immunoassay for the quantitative determination of phosphorylated p38 ([pThr180/Tyr182] p38 MAP kinase, pp38) in cell lysates. A recombinant phospho-p38 standard is provided with the kit. The assay uses a monoclonal antibody to p38 precoated in a microplate to bind the phospho-p38 in the standards or sample. After a short incubation, the excess sample or standard is washed out of the plate and a phospho p38 detection antibody is added. This antibody binds to the phospho-p38 captured on the plate. After a short incubation, the excess antibody is washed out and secondary conjugate is added, which binds to the phospho-p38 detection antibody. Excess conjugate is washed out and TMB Substrate is added. After a short incubation, the enzyme reaction is stopped and the absorbance is read at 450nm. The measured optical density is directly proportional to the concentration of phospho-p38 in either standards or samples.

Highlights:

  • Target: phosphorylated p38 ([pThr180/Tyr182] p38 MAP kinase, phospho-p38); also called SAP kinase 2 (SAPK2)
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 156 to 5000pg/mL
  • Sensitivity: < 52.1pg/mL
  • Precision: dependent upon sample type and level (see instructions)
  • Sample Types: cell lysates of human and mouse origin
  • Total Assay Time: 3 hours
  • Sample Size: 100μL
  • Specificity: no significant cross-reaction with non-phosphorylated p38, phospho-JNK, non-phosphorylated JNK, AKT, non-phosphoylated ERK, phospho-ERK.
Video of the Thermo Scientific Phospho p38 ELISA Kit

Includes:

Complete kit contains pre-coated antibody-plate, detection antibodies, buffers, standard, substrate, and stop solution.

Related Resources:

Related Products:


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
EMSP38P Phospho p38 ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Precoated 96-well Plate
Conjugate
Antibody
Assay Buffer 4 Concentrate
Wash Buffer Concentrate
Standard
TMB Substrate
RIPA Cell Lysis Buffer 2
Stop Solution 2
1-plate kit MSDS for product #EMSP38P Phospho p38 ELISA Kit $450.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

Recommend this page

Follow Us
Email Sign-up  Email Announcements

You may also need
62209 - STAT3 Colorimetric In-Cell ELISA Kit

 



Antibodies  |   Molecular Biology   |   Cell Biology   |  Thermo Scientific  |   * Trademarks  |   Privacy Statement
© 2014 Thermo Fisher Scientific Inc.   |   3747 N Meridian Rd, Rockford, IL USA 61101   |   1-800-874-3723  or  815-968-0747