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Phenol-Chloroform


Perform extractions in less time, and with fewer hazards.

Pierce Phenol-Chloroform

Thermo Scientific Pierce Phenol-Chloroform is phenol supplied premixed with chloroform:isoamyl alcohol (25:24:1) and accompanying bottle of buffer to equilibrate at either pH 6.7 or pH 8.0.

Highlights:

  • Offers excellent, nuclease-free performance
  • Phenol is supplied premixed with Chloroform:Isoamyl Alcohol (25:24:1)
  • Supplied at pH 6.7 for special, lower pH DNA extractions; for most DNA applications, the alkaline buffer (included) should be added to produce a pH of 8.0

References:

  1. Sambrook, J., et al. (1989). Molecular Cloning: A Laboratory Manual, 2nd Ed. New York: Cold Spring Harbor Press, p. 1.40.
  2. Ausubel, F.M., et al. (1992). Current Protocols in Molecular Biology. New York: Wiley Interscience, p. 2.1.1. 

Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
17908 Phenol:Chloroform + Tris Buffer
Formulation: Bottle of 25:24:1 mixture of phenol: chloroform: isoamyl alcohol, plus bottle of Tris (to adjust pH)
100mL MSDS for product #17908 Phenol:Chloroform + Tris Buffer Certificate of Analysis for product #17908 Phenol:Chloroform + Tris Buffer $59.00

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17909 Phenol:Chloroform + Tris Buffer
Formulation: Bottle of 25:24:1 mixture of phenol: chloroform: isoamyl alcohol, plus bottle of Tris (to adjust pH)
400mL MSDS for product #17909 Phenol:Chloroform + Tris Buffer Certificate of Analysis for product #17909 Phenol:Chloroform + Tris Buffer $142.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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