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Peroxidase Suppressor


A stable, easy-to-use endogenous peroxidase inhibitor.

Pierce Peroxidase Suppressor

Thermo Scientific Pierce Peroxidase Suppressor is a stable, easy-to-use inhibitor of endogenous peroxidase activity from cells and tissues to reduce background signal in HRP-based immunohistochemistry applications.

This peroxidase suppressor is optimized for immunohistochemical applications. The Peroxidase Suppressor is a stable, easy-to-use inhibitor of endogenous peroxidase activity. This proprietary formulation is the most effective peroxidase inhibitor available. The immunoperoxidase method is a commonly used detection method for immunostaining of cells and tissues. However, the analysis often is complicated by the presence of endogenous peroxidase and “pseudoperoxidase” activity in the cells and tissues. This presence results in nonspecific staining when a substrate such as diaminobenzidine (DAB) is present. Nonspecific staining is difficult to distinguish from the immunochemical marker, making it difficult to distinguish the final location of the antigen of interest. The typical method of inhibiting endogenous peroxidase activity involves hydrogen peroxide or hydrogen peroxide in methanol. The peroxidase suppressor inhibits peroxidase activity even more effectively than the method using hydrogen peroxide in methanol. Plus, the Peroxidase Suppressor does not affect antibody activity (see figure). Peroxidase Suppressor also can be used on blots of whole cell supernatant prior to the addition of the horseradish peroxidase (HRP) marker.

Highlights:

  • Stable
  • One component
  • Ready-to-use
  • Effectively inhibits peroxidase activity without interfering with antigen-antibody
    interactions

Product Details:

Relative percent inhibition
Performance of Thermo Scientific Pierce Peroxidase Suppressor.

References:

  1. Charter, N.W. (2002). J. Biol. Chem. 277: 9255-61.

Related Resources:

Immunohistochemistry (IHC) technical guide
IHC immunodetection strategies technical guide

Related Products:

Other Immunohistochemistry Reagents
Formaldehyde (16% solution)


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
35000 Peroxidase Suppressor
Formulation: Proprietary 80% methanol solution
Sufficient For: 100mL of treatment solution (1000 to 2000 tissues samples at 1 to 2 drops per sample)
100mL Product Instructions for product #35000 Peroxidase Suppressor MSDS for product #35000 Peroxidase Suppressor Certificate of Analysis for product #35000 Peroxidase Suppressor $116.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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