Thermo Scientific Pierce PDPH is a short, pyridyldithiol-and-hydrazide crosslinker for reversibly conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).
- Reactive groups: pyridyldisulfide and hydrazide
- Reactive toward: sulfhydryl groups and carbonyl (aldehyde) groups
- Short (9.2A), sulfhydryl-to-aldehyde crosslinker with disulfide bond spacer arm (cleavable)
- Pyridyldithiol group results in attachment to sulfhydryls via disulfide bond, which can be cleaved with DTT, TCEP or other reducing agents
- Hydrazide group conjugates to oxidized sugars of glycoproteins and carbohydrates
- Use sodium meta-periodate to oxidize glycosylation (e.g., sialic acid) to reactive aldehyde groups
- Use with EDC to conjugate primary amine of hydrazide group to carboxyl groups
|Chemical structure of PDPH crosslinking reagent.
- Friden, P.M., et al. (1993). Science 259, 373-377.
- Zara, J.J., et al. (1991). Anal. Biochem. 194, 156-162.
- Greenfield, R.S., et al. (1990). Evaluation in vitro of adriamycin immunoconjugates synthesized using an acid sensitive hydrazone linker. Cancer Res. 50, 6600-6607.
Crosslinker Application Guide -- search for recent literature references for this product
Overview of crosslinking
Chemistry of crosslinking
Protein glycosylation technical guide
Crosslinkers at a glance (selection guide)