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A short crosslinker for reversibly conjugating glycoprotein carbohydrates to sulfhydryl groups.

Pierce PDPH

Thermo Scientific Pierce PDPH is a short, pyridyldithiol-and-hydrazide crosslinker for reversibly conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).


  • Reactive groups: pyridyldisulfide and hydrazide
  • Reactive toward: sulfhydryl groups and carbonyl (aldehyde) groups
  • Short (9.2A), sulfhydryl-to-aldehyde crosslinker with disulfide bond spacer arm (cleavable)
  • Pyridyldithiol group results in attachment to sulfhydryls via disulfide bond, which can be cleaved with DTT, TCEP or other reducing agents
  • Hydrazide group conjugates to oxidized sugars of glycoproteins and carbohydrates
  • Use sodium meta-periodate to oxidize glycosylation (e.g., sialic acid) to reactive aldehyde groups
  • Use with EDC to conjugate primary amine of hydrazide group to carboxyl groups

Product Details:

3-(2-Pyridyldithio)propionyl hydrazide; PDPH
Chemical structure of PDPH crosslinking reagent.


  1. Friden, P.M., et al. (1993). Science 259, 373-377.
  2. Zara, J.J., et al. (1991). Anal. Biochem. 194, 156-162.
  3. Greenfield, R.S., et al. (1990). Evaluation in vitro of adriamycin immunoconjugates synthesized using an acid sensitive hydrazone linker. Cancer Res. 50, 6600-6607.

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Resources:

Overview of crosslinking
Chemistry of crosslinking
Protein glycosylation technical guide

Related Products:

Crosslinkers at a glance (selection guide)

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
22301 PDPH
Formulation: 3-(2-Pyridyldithio)propionyl hydrazide; powder solid
Sufficient For: 218mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use
50mg Product Instructions for product #22301 PDPH MSDS for product #22301 PDPH Certificate of Analysis for product #22301 PDPH $171.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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