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Cleaved PARP In-Cell ELISA Kits


Measure activated (cleaved) poly (ADP-ribose) polymerase (PARP) in whole cells.

Pierce Cleaved PARP In-Cell ELISA Kits

Thermo Scientific Pierce Cleaved PARP In-Cell ELISA Kits are colorimetric and fluorescent microplate assays optimized to measure activated (cleaved) poly (ADP-ribose) polymerase (PARP) and tubulin in whole cells.

These ELISA kits for whole cells include a rabbit monoclonal anti-(cleaved) PARP antibody, mouse monoclonal anti-alpha-Tubulin antibody, detection reagents and all buffers required for either colorimetric or near infrared detection. The Cleaved PARP In-Cell ELISA Kits enable simultaneous detection of both Cleaved PARP and Tubulin as a reference.

Highlights:

  • Measures activated (cleaved) poly (ADP-ribose) polymerase (PARP) and tubulin in whole cells using 96-well or 384-well microplates
  • Two reliable detection formats available:
    • colorimetric detection kit for use with standard microplate readers
    • near infrared fluorescent detection kit for use with IR imaging system
  • Whole-cell detection requires no cell lysis, protein extraction or special sample preparation
  • Provides better data and higher throughput than quantitative Western blotting
  • Less costly (one well vs. one lane) than Western blotting

Overview of PARP:

Poly (ADP-ribose) polymerase (PARP) plays a role in repair of single stranded DNA nicks. PARP also regulates proteins such as histones and telomerase through ADP-ribosylation and is an important marker of caspase 3-mediated apoptosis. In response to apoptotic signals, activated caspase 3 cleaves the 116 kDa PARP protein into 85 kDa and 25 kDa fragments. In humans, PARP cleavage occurs at Asp 214 and Gly 215 residues. Cleavage of PARP correlates with DNA fragmentation and other morphological changes making it a critical marker for apoptosis. The Cleaved PARP In-Cell ELISA Kit enables simultaneous detection of both Cleaved PARP and Tubulin as a reference.

Related Resources:

Overview and Menu of Target-Specific In-Cell ELISA Kits
Dylight Fluor Spectral Properties (Ex/Em)

Related Products:

ELISA reagent reservoirs and sealing tape
Secondary Antibodies and Conjugates For ELISAs and Western Blotting
DyLight Fluor Conjugated Secondary Antibodies and Biotin Binding Proteins

Notice:

This product is for In Vitro experimental use only. Not for resale without express authorization.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
62219 Cleaved PARP Colorimetric In-Cell ELISA Kit
Formulation: Multi-component kit
Sufficient For: 96 microplate wells of overnight-cultured cells

Kit Contents:
Blocking Buffer, 50mL
20X Tris Buffered Saline, 50mL
Surfact-Amps 20 (10% Detergent), 10mL
Surfact-Amps X-100 (10% Detergent), 10mL
HRP Conjugate, 0.14mL
TMB Substrate, 58mL
TMB Stop Solution, 55mL
Janus Green Whole-Cell Stain, 50mL
Elution Buffer, 2 x 30mL
Thin Plate Seal Assembly, 8 each
Anti-Cleaved PARP Antibody
Anti-alpha Tubulin Antibody
96-test kit Product Instructions for product #62219 Cleaved PARP Colorimetric In-Cell ELISA Kit MSDS for product #62219 Cleaved PARP Colorimetric In-Cell ELISA Kit $380.00

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62224 Cleaved PARP Near Infrared In-Cell ELISA Kit
Formulation: Multi-component kit
Sufficient For: 96 microplate wells of overnight-cultured cells

Kit Contents:
Blocking Buffer, 100mL
20X Tris Buffered Saline, 50mL
Surfact-Amps 20 (10% Detergent), 10mL
Surfact-Amps X-100 (10% Detergent), 10mL
DyLight 680 Goat Anti-Mouse IgG (H+L), 55µL
DyLight 800 Goat Anti-Rabbit IgG (H+L), 55µL
Thin Plate Seal Assembly, 8 each
Anti-Cleaved PARP Antibody
Anti-alpha Tubulin Antibody
96-test kit Product Instructions for product #62224 Cleaved PARP Near Infrared In-Cell ELISA Kit MSDS for product #62224 Cleaved PARP Near Infrared In-Cell ELISA Kit $380.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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