Update profile
    Change password
    Sign out

PageRuler Prestained NIR Protein Ladder


A broad-range MW marker for near-infrared (IR) fluorescence applications.

PageRuler Prestained NIR Protein Ladder (Fluorescent Molecular Weight Marker)

Thermo Scientific PageRuler Prestained NIR Protein Ladder is a mixture of 10 proteins (11 to 250KDa) that are blue-stained and fluor-labeled for near-IR fluorescent visualization and protein sizing following electrophoresis.

The protein MW markers in this ladder resolve into sharp bands when analyzed by SDS-PAGE and are labeled with a fluorescent dye for visualization with instruments equipped for detection of near-infrared (NIR) fluorescence. The bands are also directly visible because the proteins are prestained blue. The 55kDa band is of greater intensity and serves as a reference band. The protein ladder is conveniently packaged and ready to use: no heating, dilution or addition of a reducing agent is required before use.

Highlights:

  • Size range – 10 proteins spanning 11 to 250kDa
  • Fluorescent – detect using 670nm red laser or 700nm channels with fluorescence imagers
  • Ready-to-use – supplied in a loading buffer for direct loading on gels
  • Sharp bands – excellent sizing for near-infrared visualization of protein gels and blots
  • Reference band – 55kDa band has greater intensity for easy orientation
  • Quality tested – each lot evaluated by SDS-PAGE and Western blotting

Includes:

  • Proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN3 and 33% glycerol

Applications:

  • Protein sizing on SDS-polyacrylamide gels and Western blots for visualization with near-infrared (NIR) fluorescence imagers

Requires:

Fluorescent imager capable of near-infrared fluorescence detection, such as certain Typhoon™ Imagers and the LI-COR Odyssey™ Infrared Imaging System

Product Details:

SDS-PAGE band profile of the Thermo Scientific PageRuler Prestained NIR Protein Ladder.
SDS-PAGE band profile of the Thermo Scientific PageRuler Prestained NIR Protein Ladder. Images represent the same 4-20% Tris-glycine gel (SDS-PAGE) visualized directly with the unaided eye (left) and via near-infrared fluorescence lasers with the Typhoon™ Imager (GE Biosciences). Similar fluorescence imaging results are obtained with the LI-COR Odyssey™ System.

Related Resources:

Protein Molecular Weight Markers – product comparison and selection guide
Protein Gel Stains and Kits – compare colorimetric and fluorescent gel stains

Related Products:

Precise Protein Gels – browse all pre-cast gels for SDS-PAGE
BupH Dry Blend Electrophoresis Buffers – Tris-HEPES-SDS and other pouched electrophoresis buffers
Reducing Agents – 2-mercaptoethanol, DTT, TCEP and other reducing agents
Western blotting substrates and reagents – find kits, stripping buffers, blotting membranes and film


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
26635 PageRuler Prestained NIR Protein Ladder
Formulation: 10 proteins from 11 to 250kDa in reducing sample buffer
Sufficient For: 50 to 250 mini gel lanes at 1 to 5µL per lane for infrared imaging detection
2 x 250┬ÁL Product Instructions for product #26635 PageRuler Prestained NIR Protein Ladder MSDS for product #26635 PageRuler Prestained NIR Protein Ladder Certificate of Analysis for product #26635 PageRuler Prestained NIR Protein Ladder $204.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

Recommend this page

Follow Us
Email Sign-up  Email Announcements

 



Antibodies  |   Molecular Biology   |   Cell Biology   |  Thermo Scientific  |   * Trademarks  |   Privacy Statement
© 2014 Thermo Fisher Scientific Inc.   |   3747 N Meridian Rd, Rockford, IL USA 61101   |   1-800-874-3723  or  815-968-0747