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P-PER Plant Protein Extraction Reagent


Extract proteins from plant leaves, stems, roots, seeds and flowers without liquid nitrogen.

P-PER Plant Protein Extraction Kit

The Thermo Scientific P-PER Plant Protein Extraction Kit effectively extracts protein from all kinds of dry and fresh plant tissue without liquid nitrogen.

The P-PER Kit includes an organic lysing reagent and two aqueous reagents that effectively lyse plant cells and solubilize protein without harsh mechanical lysis aids, such as a mortar and pestle. Plant protein extracts are prepared in just 10 minutes, and protein yields exceed conventional extraction methods (e.g., freeze/grinding in liquid nitrogen) and other commercially available plant protein extraction reagents. The resulting protein extracts are compatible with a variety of downstream applications including the Thermo Scientific BCA Protein Assay Kit - Reducing Agent Compatible (Part No. 23250), 1D and 2D gel electrophoresis, immunoprecipitation, Western blotting, activity assays and protein purification.

Highlights:

  • Versatile – works with multiple plant organs (leaf, stem, root, seed and flowers); multiple plant species (Arabidopsis, tobacco, maize, soybeans, peas, spinach and other plant tissues); and fresh, frozen and dehydrated plant tissues
  • Convenient – requires no liquid nitrogen/freeze-grinding, Dounce homogenization, blade-shearing or glass-bead agitation for cell disruption; however, the P-PER Kit is compatible with these alternative mechanical aids
  • Compatible – downstream applications include 1-D and 2-D gel electrophoresis, Western blotting, activity assays, and protein affinity purifications
  • Quantifiable – P-PER Kit extracts can be quantified using the BCA Protein Assay Kit, Reducing Agent Compatible (Part No. 23250)
  • Ready-to-use – protein extract does not require filtration through cheesecloth or Miracloth, unlike homebrews
  • Fast – perform plant cell lysis and protein extraction in 10 minutes
  • Recovers active protein – assays show extracted proteins are functional

Product Details:

Protocol summary for the P-PER Plant Protein Extraction Kit
Protocol summary for the P-PER Plant Protein Extraction Kit.

High-yield plant protein extraction with P-PER Kit
The P-PER Plant Protein Extraction Kit produces equivalent or higher levels of extracted protein than traditional and other commercial methods. Fresh leaf tissue from tobacco, maize seedlings and Arabidopsis were lysed and extracted according to the P-PER Kit protocol, a competitor protocol and a literature-based (homebrew) protocol. Samples were normalized (weight tissue/volume extract), resolved on a 10% Bis-Tris gel and stained with Imperial Protein Stain (Part No. 24615). Samples were also quantified using the BCA Protein Assay Kit, Reducing Agent Compatible (Part No. 23250). Panel A. Lane 1: Molecular weight standards, Lanes 2-4: tobacco leaves, Lanes 5-7: corn leaves and Lanes 8-10: Arabidopsis. Panel B. Lanes 1-2: dehydrated soybean seed and Lanes 3-4: dehydrated corn kernel. Note: The Competitor S method is recommended for leaf tissue only. The extracted protein levels and the ratios of extracted protein per total plant tissue weight were determined for all samples.

Plant extraction reagent is compatible with common grinding devices.
Effective plant protein extraction using common mechanical grinding aids. Fresh tobacco leaf tissue was extracted with Thermo Scientific P-PER Reagent Working Solution using various plant tissue grinding aids. Samples were normalized (weight tissue/volume extract), resolved on a 4-12% Bis-Tris gel and stained with Imperial Protein Stain (Part No. 24615). Samples were also quantified using the BCA Protein Assay Kit, Reducing Agent Compatible (Part No. 23250). Lane 1: Molecular weight marker, Lane 2: mesh bag, Lane 3: Wheaton glass Dounce homogenizer, Lane 4: liquid nitrogen/mortar and pestle grind, Lane 5: Polytron Tissue Grinder, Lane 6: BioMasher Sample Prep Device (Cartagen) and Lane 7: blue polypropylene pestle (Kontes).

Plant protein extracts for 2-D gel electrophoresis
The P-PER Kit is compatible with 2-D gel electrophoresis. Protein was extracted from 160mg of Arabidopsis rosette leaves using the P-PER Kit. Samples were focused on pH 3-10 nonlinear IPG strips followed by 8-16% SDS-PAGE. The gels were stained with a fluorescent stain.

Related Resources:

P-PER Kit FAQ

Related Products:

Fisher BioReagents™ Polyvinylpyrrolidone (PVP) – phenolic inhibitor (see FAQ)


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
89803 P-PER Plant Protein Extraction Reagent
Formulation: Multi-component kit
Sufficient For: Total of 400mg dried or 1.6g fresh plant tissue

Kit Contents:
P-PER Reagent A, 20mL
P-PER Reagent B, 225µL
P-PER Reagent C, 20mL
Polypropylene Mesh Bags, 20 each
40mL kit Product Instructions for product #89803 P-PER Plant Protein Extraction Reagent MSDS for product #89803 P-PER Plant Protein Extraction Reagent Certificate of Analysis for product #89803 P-PER Plant Protein Extraction Reagent $274.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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