Thermo Scientific Pierce p-Amino Phosphoryl Choline Agarose enables purification of C-reactive protein (CRP).
The immobilized phosphoryl choline is a beaded agarose affinity resin for purification of C-reactive protein (CRP) from plasma, ascites and other biological fluids. CRP is a pentameric (pentraxin) protein that is classified as an acute phase reactant because of its high level of production during inflammation. The protein binds to phosphoryl choline groups on the surface of microbes and has been linked to several biological functions including activation of the classical complement pathway, enhancement of phagocytosis and interaction with certain subpopulations of T-lymphocytes. In the late 1970s, Volanakis and colleagues discovered a method of isolating and studying C-reactive protein by using the protein's affinity for phosphoryl choline that has been immobilized to a porous solid chromatography support.
- Ligand: p-aminophenyl phosphoryl choline
- Support: crosslinked 6% beaded agarose
- Binding capacity: greater than 3mg of human C-reactive protein per milliliter of settled resin
- Format: 50% resin slurry in 0.02% sodium azide
- Binding condition: Tris or borate-buffered saline (pH 8 to 8.5) with 1 to 2mM calcium chloride
- Elution condition: Tris or borate-buffered saline (pH 8 to 8.5) with 2mM EDTA
- Reusable: resin can be regenerated and reused several times
|Chemical structure of Thermo Scientific Pierce p-Aminophenyl Phosphoryl Choline Agarose. Each porous agarose bead is 45 to 165µm diameter and contains many billions of p-aminophenyl phosphoryl choline groups.
Review of affinity purification
- Robey, F.A. and Liu, T.Y. (1981). J. Biol. Chem. 256, 969-975.
- Volanakis, J.E. et al. (1978). J. Immunol. Meth. 23:285-95.
- McGrath, F.D.G., et al.(2006). J. Immunol. 176:2950-2957.
- Riley-Vargas, R.C., et al.(2005). J. Clin. Invest. 115:1241-1249.
- Robey, F.A. and Liu, T.Y. (1981). J. Biol. Chem. 256:969-975.
- vanden Berg, C.W., et al.(2004). Arterioscler. Thromb. Vasc. Biol. 24:e168-e171.