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OPD (o-Phenylenediamine)

HRP substrate that produces a soluble yellow-orange end-product.

Pierce OPD

Thermo Scientific Pierce OPD is a water-soluble substrate for horseradish peroxidase (HRP) that produces a yellow-orange product detectable at 492nm by ELISA plate readers.

OPD yields a water soluble yellow-orange product when reacted with peroxidase with an absorbance maximum of 492. OPD can easily be dissolved in a substrate buffer such as Stable Peroxide Substrate Buffer (Part No. 34062).

Related Resources:

Assay development and ELISA technical guide
ELISA substrate selection guide

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
34005 OPD
Formulation: o-phenylenediamine dihydrochloride; powder (at least 98% pure)
Sufficient For: 50L of typical 1X (5mg/10mL) substrate solution
25g Product Instructions for product #34005 OPD MSDS for product #34005 OPD Certificate of Analysis for product #34005 OPD $67.00

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34006 OPD Tablets
Formulation: Tablets each containing 5mg of OPD with inert filler as the balance
Sufficient For: 10mL of typical 1X substrate solution per tablet (500mL total)
50 tablets Product Instructions for product #34006 OPD Tablets MSDS for product #34006 OPD Tablets Certificate of Analysis for product #34006 OPD Tablets $139.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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34062 - Stable Peroxide Substrate Buffer (10X)

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