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ONPG Substrate


Detect beta-galactosidase probes in ELISA applications.

ONPG for beta-galactosidase detection

Thermo Scientific Pierce ONPG is the preferred colorimetric substrate for ELISA applications involving beta-galactosidase (b-Gal) as the reporter enzyme.

When using beta-Galactosidase as the label for proteins in ELISA studies, a wide variety of substrates are available, including: o-Nitrophenyl-beta-D-galactopyranosidase (ONPG), Naphthol-AS-Bl-beta-D-galactopyranosidase (Nap-Gal), and 4-Methyl-umbelliferyl-beta-D-galactopyranosidase (MUm-Gal). However, it is important to choose a substrate with adequate solubility, that uses readily available equipment, and that gives a significant reading over the background. ONPG is a superior beta-Galactosidase substrate option.(1) The product formed is completely soluble and has a high extinction coefficient at 405nm. The substrate yields a yellow product that is easily detectable in the visual range after stopping the reaction with 1M Sodium Carbonate.

References:

  1. Craven, G.R., Steers, Jr., E., and Anfinsen, C.B.,(1965). Purification, composition and molecular weight of the beta-Galactosidase of Escherichia coli K12. J. Biol. Chem. 240, 2468-2477.

Related Resources:

Assay development and ELISA technical guide
ELISA substrate selection guide


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
34055 ONPG
Formulation: o-nitrophenyl-β-D-galactopyranoside; powder
Sufficient For: At least 5L of typical 1X (3mM) substrate solution
5g Product Instructions for product #34055 ONPG MSDS for product #34055 ONPG Certificate of Analysis for product #34055 ONPG $82.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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