Thermo Scientific Octyl-beta-Glucoside is a nonionic detergent that has been widely used for membrane protein solubilization.
- A low-molecular weight, nonionic detergent
- Effective for membrane protein solubilization
- Less stable and less commonly used than octylthioglucoside (OTG)
- Can be removed from solution by dialysis
- Optically clear; low absorbance at 280nm
|Chemical structure of N-Octyl-β-D-glucoside detergent.
Properties of N-Octyl-β-D-glucoside:
- Alternative Names: Octyl-beta-glucoside, OG, Octyl-beta-glucopyranoside, Octyl-beta-D-glucopyranoside
- Chemical Name: n-Octyl-β-D-glucoside
- Molecular Weight: 292.37g
- Detergent Class: Nonionic
- Aggregation Number: 27
- Micelle Molecular Weight: 8000g
- Critical Micelle Concentration (CMC): 23 to 25mM (0.6716 to 0.7300%, w/v)
- Cloud Point: >100°C
- Dialyzable: Yes
Specifications for Octyl-beta-Glucoside (Part No. 28309, 28310):
- Visual: White, free-flowing powder, free of foreign material.
- Solubility: A 20% (D.I. water) solution must be clear, colorless, with no particulates.
- Absorbance (400nm) of a 20% solution: <0.025
- Absorbance (280nm) of a 20% solution: <0.300
- Specific Rotation (20°C, 1%): -29.0 to -32.0°C
- Identity: The IR scan must show only peaks characteristic for the structure and functional groups of the compound being tested.
- Rosevear, P., et al. (1980). Biochemistry 19, 4108-4115.
- Gould, R.J., et al. (1981). Biochemistry 20, 6776-6781.
- Jackson, M.L., et al. (1982). Biochemistry 21, 4576-4582.
Review of detergents for cell lysis
Detergent Sampler Pack
Other Detergents for Protein Methods
Detergent Removal Resins