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Nylon Membranes


Near-neutral and cationic membranes especially for molecular biology blots.

Biodyne A and Biodyne B Nylon Membranes

Thermo Scientific Biodyne A and Biodyne B Nylon Membranes are sheets of unmodified or positively charged Nylon that provide excellent binding characteristics for nucleic acid transfer, blotting and detection methods.

These two varieties of nylon membrane are manufactured with a similar matrix pore size (0.4 micron), and both are supplied in packages of 8 x 12cm sheets. The Biodyne A Membrane contains both negatively and positively charged groups, enabling it to bind many kinds of macromolecules. The Biodyne B Membrane contains exclusively positive charges, providing the highest possible binding capacity for negatively charged molecules like DNA and RNA. The membranes are resistant to heat and solvents and will not shrink, crack or tear.

Highlights:

  • Versatile and durable – achieve strong DNA binding by baking or UV-crosslinking without damaging the membrane
  • Easy to use – pre-cut 8 x 12cm sheets are the ideal size for standard mini-gel transfers and microplate dot-blots
  • High signal-to-noise ratio – lower background than nitrocellulose and most other nylon transfer membranes

Properties of Biodyne A Nylon Membrane:

  • Composition: unmodified Nylon 66
  • Surface: 50% amine and 50% carboxyl groups, pI 6.5
  • Properties: provides excellent resolution, high sensitivity and extremely low background

Properties of Biodyne B Nylon Membrane:

  • Composition: positively charged Nylon 66
  • Surface: strong cationic quaternary ammonium groups provide positive zeta potential, even to pH 10
  • Properties: excellent for DNA, RNA and other negatively charged molecules
  • Applications: Southern blotting, Northern blotting, DNA and RNA methods, including gel shift assays with Thermo Scientific LightShift Chemiluminescent EMSA Kits

Product Details:

More Product Data

Related Products:

LightShift Chemiluminescent DNA EMSA Kit
Nucleic Acid Labeling and Detection Products
CL-Xposure Film – X-ray film for autoradiography and chemiluminecence


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
77015 Biodyne A Nylon Membrane, 0.45µm, 8cm x 12cm
Formulation: Surface contains 50% amino and 50% carboxyl groups
25 sheets Product Instructions for product #77015 Biodyne A Nylon Membrane, 0.45µm, 8cm x 12cm $342.00

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77016 Biodyne B Nylon Membrane, 0.45µm, 8cm x 12cm
Formulation: Surface modified with positive zeta potential
25 sheets Product Instructions for product #77016 Biodyne B Nylon Membrane, 0.45µm, 8cm x 12cm Certificate of Analysis for product #77016 Biodyne B Nylon Membrane, 0.45µm, 8cm x 12cm $249.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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