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North2South Chemiluminescent Detection Kits


High-sensitivity Southern blotting without radioactivity.

North2South Chemiluminescent Nucleic Acid Detection Kits

Thermo Scientific North2South Chemiluminescent Detection Kit combines a novel ECL substrate for horseradish peroxidase (HRP) and optimized hybridization and blocking buffers to ensure consistent, high-sensitivity Southern blot results.

The North2South Hybridization and Detection Kit combines a novel enhanced luminol substrate for horseradish peroxidase (HRP) with optimized pre-made hybridization and blocking buffers to ensure consistent results and high sensitivity. Any nucleic acid blot can be hybridized with the appropriate biotinylated (biotin-labeled) oligonucleotide probe. After a brief membrane blocking step, the biotin label is detected with streptavidin- HRP and a 5-minute incubation with the chemiluminescent substrate. Results are easily recorded by brief exposure to X-ray film (1-15 minutes) or CCD camera.

Highlights:

  • Sensitivity equal to or greater than 32P
  • Much faster than DIG detection systems
  • Amenable to nearly any DNA or RNA hybridization method
  • Optimized hybridization and blocking buffers and protocol steps yield consistent results from experiment to experiment
  • High level of light output and consistent low background allows for very short and multiple exposure times using cooled CCD camera systems or film
  • Ready-to-use buffers and short process time makes switching from radioactive detection to chemiluminescent detection an easy transition, even for the novice user
  • Specific plaques can be picked out of areas that are very dense with signal due to extremely low background

Product Details:

North2South Kit for Southern blotting and DNA fingerprinting. Detection of biotinylated DNA on a Southern blot using the North2South System and a cooled CCD imaging system. A Southern blot of biotinylated DNA samples from a DNA fingerprinting kit was detected using the North2South System and imaged for one minute using a cooled CCD camera system.

References:

  1. Adilakshmi, T. and Laine, R.O. (2002). J. Biol. Chem. 277(6), 4147-4151.
  2. Bach, S., et al. (2002). Infect. Immun. 70(2), 988-992.
  3. Borst, E-M., et al. (1999). J. Virol. 73(10), 8320-8329.
  4. Haertel-Wiesmann, M., et al. (2000). J. Biol. Chem. 275(41), 32046-32051.

Related Resources:

North2South Kit FAQ

Related Products:

North2South Biotin Random Prime DNA Labeling Kit


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
17097 North2South Chemiluminescent Hybridization and Detection Kit
Formulation: Multi-component kit with reagents for hybridization and detection of Northern or Southern blots
Sufficient For: 1000cm2 of membrane (10 mini-gel blots)

Kit Contents:
Stabilized Streptavidin-HRP, 1.5mL
Luminol/Enhancer Solution, 80mL
Stable Peroxide Solution, 80mL
Hybridization Buffer, 125mL
Hybridization Stringency Wash Buffer (2X), 375mL
Blocking Buffer, 500mL
Wash Buffer (4X), 500mL
Substrate Equilibration Buffer, 500mL
10-blot kit Product Instructions for product #17097 North2South Chemiluminescent Hybridization and Detection Kit MSDS for product #17097 North2South Chemiluminescent Hybridization and Detection Kit $448.00

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17175 North2South Complete Biotin Random Prime Labeling and Detection Kit
Formulation: Multi-component kit with reagents for DNA labeling, hybridization and detection of Northern or Southern blots
Sufficient For: 10 biotin-labeling reactions (each with 100ng DNA) and 10 mini-gel blots

Kit Contents:
North2South Chemiluminescent Hybridization and Detection Kit (Part No. 17097), 1 kit
North2South Biotin Random Prime Labeling Kit (Part No. 17075), 1 kit
10-blot kit Product Instructions for product #17175 North2South Complete Biotin Random Prime Labeling and Detection Kit MSDS for product #17175 North2South Complete Biotin Random Prime Labeling and Detection Kit $573.00

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17295 North2South Chemiluminescent Substrate for HRP
Formulation: Proprietary luminol and peroxide solutions
Sufficient For: 1000cm2 of membrane (10 mini-gel blots)

Kit Contents:
Luminol/Enhancer Solution, 50mL
Stable Peroxide Solution, 50mL
100mL kit Product Instructions for product #17295 North2South Chemiluminescent Substrate for HRP MSDS for product #17295 North2South Chemiluminescent Substrate for HRP Certificate of Analysis for product #17295 North2South Chemiluminescent Substrate for HRP $305.00

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37549 North2South Hybridization Buffer
Formulation: Proprietary aqueous buffer, pH 6.9
Sufficient For: 1000cm2 of membrane (10 mini-gel blots)
125mL MSDS for product #37549 North2South Hybridization Buffer Certificate of Analysis for product #37549 North2South Hybridization Buffer $124.00

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37555 North2South Hybridization Stringency Wash Buffer (2X)
Formulation: Upon dilution with equal volume of water, solution yields 60mM sodium citrate, 0.6M NaCl, 0.2% SDS, pH 7.9
Sufficient For: 1000cm2 of membrane (10 mini-gel blots)
375mL MSDS for product #37555 North2South Hybridization Stringency Wash Buffer (2X) Certificate of Analysis for product #37555 North2South Hybridization Stringency Wash Buffer (2X) $111.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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