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The reagent of choice for colorimetric detection of amino acids.

Pierce Ninhydrin

Thermo Scientific Pierce Ninhydrin is a classic, amine-reactive, colorimetric detection reagent for protein detection and amino acid analysis.


  • For post-column amino acid derivatization for colorimetric detection with millimolar sensitivity
  • Reacts with all primary amine-containing analytes to yield colored derivatives (λmax = 440nm)
  • Stable indefinitely; no refrigeration required; keep bottle tightly sealed; avoid exposure to direct sunlight and ammonia

Reagent Properties:

  • Alternative names: Ninhydrin
  • Chemical name: 2,2-Dihydroxy-1H-indene-1,3(2H)-dione
  • Reactive toward: Primary amines (–NH2); some secondary amines
  • Chemical formula: C9H6O4
  • CAS number: 485-47-2
  • Molecular weight: 178.14
  • Form: Pale to light yellow-green, free-flowing crystals

Product Details:

Chemical structure of Ninhydrin Chemical structure of ninhydrin.


  1. Stein, W.H. and Moore, S. (1949). Cold Spring Harbor Symp. Quant. Biol. 14, 179.
  2. Moore, S. (1968). Amino acid analysis: aqueous dimethyl sulfoxide as solvent for the ninhydrin reaction. J. Biol. Chem. 243(23), 6281-6283.
  3. James, L.B. (1978). Amino acid analysis: ninhydrin reaction with titanous chloride. J. Chrom. 152, 298-300.

Related Products:


Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
21003 Ninhydrin
Formulation: 2,2-Dihydroxyindane-1,3-dione; crystalline powder
Sufficient For: Many experiments, depending on assay system
500g MSDS for product #21003 Ninhydrin Certificate of Analysis for product #21003 Ninhydrin $804.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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