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Pierce Nickel Coated Plates


Capture His-tagged fusion proteins for easy plate-based assays.

Pierce Nickel Coated Plates

Thermo Scientific Pierce Nickel Coated Plates provide a simple format to bind His-tagged fusion proteins for ELISA and other plate-based assays involving protein interactions of expressed recombinant proteins.

These Ni(2+) chelate-coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking. The clear, white or black plates can be used with colorimetric, chemiluminescent or fluorescent detection methods, respectively.

Highlights:

  • Ni2+ activated surface enables metal-chelate binding of His-tagged proteins
  • Detergents used to lyse cells don't inhibit binding to activated plates as they do with plain polystyrene
  • Better binding for sensitive assays compared to other commercially available nickel-activated plates
  • Detection limit: 1ng of polyhistidine fusion protein
  • Binding capacity: approx. 9pmol His-tagged protein (27kDa) per well
  • Activation level: 200µL

Product Details:

Binding performance of nickel coated plates. Comparison of Thermo Scientific Pierce Nickel Coated Plates (Pierce) to Nickel-chelate plates from Brand Q in binding performance for His-tagged ATF fusion protein. Detection was accomplished with an HRP-conjugated anti-ATF antibody and TMB substrate. His-tagged protein binding comparison of nickel coated microplates.

References:

  1. Desai, S., et al. (1997). Previews 1(4), 12-15.
  2. Tu, Y., et al. (1999). Mol. Cell. Biol. 19, 2425-2434.
  3. Pan, W., et al. (2003). J. Biol. Chem. 278, 27820-27827.

Related Resources:

Assay development and ELISA technical guide
His-tagged proteins technical guide

Related Products:

Copper Coated Plates
Resins for His-tagged Protein Purification


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
15142 Nickel Coated Plates, Clear, 8-Well Strip
Formulation: Clear polystyrene 96-well strip plates coated and blocked at 200μL/well with proprietary nickel-presenting molecular solution containing bovine serum albumin.
Sufficient For: Binding approx. 9pmol His-tagged protein per well
5 plates Product Instructions for product #15142 Nickel Coated Plates, Clear, 8-Well Strip MSDS for product #15142 Nickel Coated Plates, Clear, 8-Well Strip Certificate of Analysis for product #15142 Nickel Coated Plates, Clear, 8-Well Strip $205.00

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15242 Nickel Coated Plates, White, 96-Well
Formulation: White polystyrene 96-well plates coated and blocked at 200μL/well with proprietary nickel-presenting molecular solution containing bovine serum albumin.
Sufficient For: Binding approx. 9pmol His-tagged protein per well
5 plates Product Instructions for product #15242 Nickel Coated Plates, White, 96-Well MSDS for product #15242 Nickel Coated Plates, White, 96-Well Certificate of Analysis for product #15242 Nickel Coated Plates, White, 96-Well $205.00

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15342 Nickel Coated Plates, Black, 96-Well
Formulation: Black polystyrene 96-well plates coated and blocked at 200μL/well with proprietary nickel-presenting molecular solution containing bovine serum albumin.
Sufficient For: Binding approx. 9pmol His-tagged protein per well
5 plates Product Instructions for product #15342 Nickel Coated Plates, Black, 96-Well MSDS for product #15342 Nickel Coated Plates, Black, 96-Well Certificate of Analysis for product #15342 Nickel Coated Plates, Black, 96-Well $205.00

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15442 Nickel Coated Plates, Clear, 96-Well
Formulation: Clear polystyrene 96-well plates coated and blocked at 200μL/well with proprietary nickel-presenting molecular solution containing bovine serum albumin.
Sufficient For: Binding approx. 9pmol His-tagged protein per well
5 plates Product Instructions for product #15442 Nickel Coated Plates, Clear, 96-Well MSDS for product #15442 Nickel Coated Plates, Clear, 96-Well Certificate of Analysis for product #15442 Nickel Coated Plates, Clear, 96-Well $196.00

Add to cart
15442B Nickel Coated Plates, Clear, 96-Well
Formulation: Clear polystyrene 96-well plates coated and blocked at 200μL/well with proprietary nickel-presenting molecular solution containing bovine serum albumin.
Sufficient For: Binding approx. 9pmol His-tagged protein per well
Custom MSDS for product #15442B Nickel Coated Plates, Clear, 96-Well

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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