Update profile
    Change password
    Sign out

NHS-Phosphine Staudinger Reagent


Amine-reactive phosphine reagent for azide-phosphine chemoselective ligation.

NHS-Phosphine and Sulfo-NHS-Phosphine

Thermo Scientific NHS-Phosphine is an amine-reactive compound that can be used to derivatize primary amines of proteins or amine-coated polymer surfaces for ligation to azide-tagged molecules.

This NHS-ester compound reacts to form covalent bonds with primary amines (e.g., side chain of lysine residues or aminosilane-coated surfaces). The phosphine group (PPh2) reacts with azide-labeled molecules by a mechanism known as Staudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples. In combination with different azide-tagged compounds, this NHS-Phosphine reagent facilitates a variety of labeling and crosslinking experimental strategies for investigating protein interactions and cellular pathways.

Highlights:

  • Soluble – reagent easily dissolves in water-miscible solvents for subsequent dilution in aqueous reaction mixtures with cell lysates and other biological samples
  • Compatible – reaction chemistry occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents
  • Specific – NHS esters are specific for covalent attachment to primary amines (side chain of lysine or N-terminus of polypeptides)
  • Chemoselective – azide and phosphine groups do not react or interfere with components of biological samples (bioorthogonal) but conjugate to one another with high efficiency

Product Details:

Amine-reactive NHS-Phosphine
Chemical structure of NHS-activated (amine-reactive) phosphine ligation reagent. This reagent labels proteins and molecules that contain primary amines (e.g., side-chain of lysine) via NHS Ester Reaction Chemistry. Labeled molecules will contain a phosphine group (P3), which can be conjugated to azide-activated molecules via the Staudinger Ligation Reaction Chemistry.

Properties of NHS-Phosphine.
  NHS-Phosphine
Molecular formula C25H20NO6P
Molecular weight 461.40
Spacer arm length 5.4 angstroms
Form Yellow to yellow-orange powder
SMILES structures O=C(ON1C(CCC1=O)=O)C2=CC(P(C3=CC=CC=C3)C4=CC=CC=C4)=C(C(OC)=O)C=C2
Storage conditions -20°C with desiccant, protect from moisture, use only fresh solutions
Reactive groups N-hydroxysuccinimide ester, reacts with primary amines at pH 7.0-9.0;
Phosphine, reacts with azide groups

Related Resources:

Azide-Phosphine Reagent Guide
Review of Metabolic Labeling for Chemoselective Ligation
Review of Staudinger Ligation Reaction Chemistry
Superiority of Azide-Phosphine Chemistry

Related Products:

Azide-Sugars for Metabolic Labeling and Detection
NHS-Azide Staudinger Ligation Reagents
Phosphine-activated DyLight Fluors
Biotin-PEG3-Phosphine


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
88900 NHS-Phosphine
Formulation: Phosphine functionalized as N-hydroxysuccinimide ester; powder solid
Sufficient For: 22mL of typical 1X (1mM) reaction solution, or approx. 5 uses at 2mg reagent per use
10mg Product Instructions for product #88900 NHS-Phosphine MSDS for product #88900 NHS-Phosphine Certificate of Analysis for product #88900 NHS-Phosphine $300.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

Recommend this page

Follow Us
Email Sign-up  Email Announcements

You may also need
78833 - NE-PER Nuclear and Cytoplasmic Extraction Reagents

23228 - BCA Protein Assay Reagent A

21580 - BS3

34095 - SuperSignal West Femto Maximum Sensitivity Substrate

87723 - Slide-A-Lyzer G2 Dialysis Cassettes, 3.5K MWCO, 3mL

 



Antibodies  |   Molecular Biology   |   Cell Biology   |  Thermo Scientific  |   * Trademarks  |   Privacy Statement
© 2014 Thermo Fisher Scientific Inc.   |   3747 N Meridian Rd, Rockford, IL USA 61101   |   1-800-874-3723  or  815-968-0747