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NeutrAvidin Coated Plates - Standard Capacity


Pre-blocked and ready to capture biotinylated antibodies and other molecules.

Pierce NeutrAvidin Coated Plates

Thermo Scientific NeutrAvidin Protein Coated Plates are pre-blocked and ready-to-use polystyrene 96-well and 384-well plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays.

These Thermo Scientific Pierce Coated Plates are available in 96-well and 384-well plate formats, pre-blocked with BSA or SuperBlock Blocking Buffers. Choose from clear, white and black plates for use with colorimetric (chromogenic), chemiluminescent and fluorescent detection methods, respectively.

Highlights:

  • Easy and gentle immobilization of biotin-containing conjugates
  • Low nonspecific binding
  • No denaturing of the protein component of a conjugate upon binding
  • Ideal for binding small biotinylated hydrophilic molecules (e.g., peptides) that typically exhibit poor binding to polystyrene
  • Pre-blocked with your choice of Blocker BSA or SuperBlock Blocking Buffer
  • Less expensive than streptavidin coated plates
  • Lowest nonspecific binding properties of all biotin-binding proteins

Product Details:

Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Streptavidin is the most popular and widely available biotin-binding protein for research methods. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions. Thermo Scientific NeutrAvidin Protein is a deglycosylated form of avidin, which has a near-neutral pI (6.3), resulting in even lower nonspecific interactions. NeutrAvidin Protein has the additional advantage of lacking the bacterial RYD sequence found on streptavidin, which eliminates off-target binding to the RGD binding domain of adhesion receptors present in a variety of cells.

Plate Characteristics for Standard Capacity Biotin-Binding Plates. The coating and blocking volumes listed here are the most common varieties. Individual Part Nos. may be different.
Biotin-Binding
Protein
Plate Type Coat Volume Block Volume Approx. Binding Capacity
NeutrAvidin
(current page)
96-well Strip 100µL/well 200µL/well ~15pmol biotin/well
384-well 50µL/well 100µL/well ~10pmol biotin/well
Streptavidin
(visit page)
96-well Strip 100µL/well 200µL/well ~5pmol biotin/well
384-well 50µL/well 100µL/well ~4pmol biotin/well

Related Resources:

Avidin-biotin systems technical guide
Assay development and ELISA technical guide

Related Products:

Other Streptavidin and NeutrAvidin Coated Microplates
Blocking Buffers
ELISA Substrates

Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
15129 NeutrAvidin Coated Plates, Clear, 96-Well
Formulation: Clear polystyrene 96-well plates coated at 100µL/well with NeutrAvidin Protein and blocked at 200µL/well with SuperBlock Blocking Buffer.
Sufficient For: Binding approx. 15pmol D-biotin per well
5 plates Product Instructions for product #15129 NeutrAvidin Coated Plates, Clear, 96-Well MSDS for product #15129 NeutrAvidin Coated Plates, Clear, 96-Well Certificate of Analysis for product #15129 NeutrAvidin Coated Plates, Clear, 96-Well $155.00

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15129B NeutrAvidin Coated Plates, Clear, 96-Well
Formulation: Clear polystyrene 96-well plates coated at 100μL/well with NeutrAvidin Protein and blocked at 200μL/well with SuperBlock Blocking Buffer.
Sufficient For: Binding approx. 15pmol D-biotin per well
Custom MSDS for product #15129B NeutrAvidin Coated Plates, Clear, 96-Well
15127 NeutrAvidin Coated Plates, Clear, 8-Well Strip
Formulation: Clear polystyrene 96-well strip plates coated at 100µL/well with NeutrAvidin Protein and blocked at 200µL/well with SuperBlock Blocking Buffer.
Sufficient For: Binding approx. 15pmol D-biotin per well
5 plates Product Instructions for product #15127 NeutrAvidin Coated Plates, Clear, 8-Well Strip MSDS for product #15127 NeutrAvidin Coated Plates, Clear, 8-Well Strip Certificate of Analysis for product #15127 NeutrAvidin Coated Plates, Clear, 8-Well Strip $169.00

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15400 NeutrAvidin Coated Plates, Clear, 384-Well
Formulation: Clear polystyrene 384-well plates coated at 50μL/well with NeutrAvidin Protein and blocked at 100μL/well with SuperBlock Blocking Buffer.
Sufficient For: Binding approx. 12pmol D-biotin per well
5 plates Product Instructions for product #15400 NeutrAvidin Coated Plates, Clear, 384-Well MSDS for product #15400 NeutrAvidin Coated Plates, Clear, 384-Well Certificate of Analysis for product #15400 NeutrAvidin Coated Plates, Clear, 384-Well $322.00

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15116 NeutrAvidin Coated Plates, White, 96-Well
Formulation: White polystyrene 96-well plates coated at 100μL/well with NeutrAvidin Protein and blocked at 200μL/well with SuperBlock Blocking Buffer.
Sufficient For: Binding approx. 15pmol D-biotin per well
5 plates Product Instructions for product #15116 NeutrAvidin Coated Plates, White, 96-Well MSDS for product #15116 NeutrAvidin Coated Plates, White, 96-Well Certificate of Analysis for product #15116 NeutrAvidin Coated Plates, White, 96-Well $169.00

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15117 NeutrAvidin Coated Plates, Black, 96-Well
Formulation: Black polystyrene 96-well plates coated at 100μL/well with NeutrAvidin Protein and blocked at 200μL/well with SuperBlock Blocking Buffer.
Sufficient For: Binding approx. 15pmol D-biotin per well
5 plates Product Instructions for product #15117 NeutrAvidin Coated Plates, Black, 96-Well MSDS for product #15117 NeutrAvidin Coated Plates, Black, 96-Well Certificate of Analysis for product #15117 NeutrAvidin Coated Plates, Black, 96-Well $169.00

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15401 NeutrAvidin Coated Plates, White, 384-Well
Formulation: White polystyrene 384-well plates coated at 50μL/well with NeutrAvidin Protein and blocked at 100μL/well with SuperBlock Blocking Buffer.
Sufficient For: Binding approx. 12pmol D-biotin per well
5 plates Product Instructions for product #15401 NeutrAvidin Coated Plates, White, 384-Well MSDS for product #15401 NeutrAvidin Coated Plates, White, 384-Well Certificate of Analysis for product #15401 NeutrAvidin Coated Plates, White, 384-Well $322.00

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15402 NeutrAvidin Coated Plates, Black, 384-Well
Formulation: Black polystyrene 384-well plates coated at 50μL/well with NeutrAvidin Protein and blocked at 100μL/well with SuperBlock Blocking Buffer.
Sufficient For: Binding approx. 12pmol D-biotin per well
5 plates Product Instructions for product #15402 NeutrAvidin Coated Plates, Black, 384-Well MSDS for product #15402 NeutrAvidin Coated Plates, Black, 384-Well Certificate of Analysis for product #15402 NeutrAvidin Coated Plates, Black, 384-Well $322.00

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15123 NeutrAvidin Coated Plates, Clear, 96-Well
Formulation: Clear polystyrene 96-well plates coated at 200μL/well with NeutrAvidin Protein and blocked at 300μL/well with Blocker BSA Buffer.
Sufficient For: Binding greater than 15pmol D-biotin per well
5 plates Product Instructions for product #15123 NeutrAvidin Coated Plates, Clear, 96-Well MSDS for product #15123 NeutrAvidin Coated Plates, Clear, 96-Well Certificate of Analysis for product #15123 NeutrAvidin Coated Plates, Clear, 96-Well $155.00

Add to cart
15123B NeutrAvidin Coated Plates, Clear, 96-Well
Formulation: Clear polystyrene 96-well plates coated at 200μL/well with NeutrAvidin Protein and blocked at 300μL/well with Blocker BSA Buffer.
Sufficient For: Binding greater than 15pmol D-biotin per well
Custom MSDS for product #15123B NeutrAvidin Coated Plates, Clear, 96-Well Certificate of Analysis for product #15123B NeutrAvidin Coated Plates, Clear, 96-Well
15128 NeutrAvidin Coated Plates, Clear, 8-Well Strip
Formulation: Clear polystyrene 96-well strip plates coated at 200μL/well with NeutrAvidin Protein and blocked at 300μL/well with Blocker BSA Buffer.
Sufficient For: Binding greater than 15pmol D-biotin per well
5 plates Product Instructions for product #15128 NeutrAvidin Coated Plates, Clear, 8-Well Strip MSDS for product #15128 NeutrAvidin Coated Plates, Clear, 8-Well Strip Certificate of Analysis for product #15128 NeutrAvidin Coated Plates, Clear, 8-Well Strip $169.00

Add to cart
15128B NeutrAvidin Coated Plates, Clear, 8-Well Strip
Formulation: Clear polystyrene 96-well strip plates coated at 200µL/well with NeutrAvidin Protein and blocked at 300µL/well with Blocker BSA Buffer.
Sufficient For: Binding greater than 15pmol D-biotin per well
Custom MSDS for product #15128B NeutrAvidin Coated Plates, Clear, 8-Well Strip Certificate of Analysis for product #15128B NeutrAvidin Coated Plates, Clear, 8-Well Strip
15216 NeutrAvidin Coated Plates, White, 96-Well
Formulation: White polystyrene 96-well plates coated at 200μL/well with NeutrAvidin Protein and blocked at 300μL/well with Blocker BSA Buffer.
Sufficient For: Binding greater than 15pmol D-biotin per well
5 plates Product Instructions for product #15216 NeutrAvidin Coated Plates, White, 96-Well MSDS for product #15216 NeutrAvidin Coated Plates, White, 96-Well Certificate of Analysis for product #15216 NeutrAvidin Coated Plates, White, 96-Well $169.00

Add to cart
15217 NeutrAvidin Coated Plates, Black, 96-Well
Formulation: Black polystyrene 96-well plates coated at 200μL/well with NeutrAvidin Protein and blocked at 300μL/well with Blocker BSA Buffer.
Sufficient For: Binding greater than 15pmol D-biotin per well
5 plates Product Instructions for product #15217 NeutrAvidin Coated Plates, Black, 96-Well MSDS for product #15217 NeutrAvidin Coated Plates, Black, 96-Well Certificate of Analysis for product #15217 NeutrAvidin Coated Plates, Black, 96-Well $169.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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