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myECL Imager


One-touch image capture and analysis for protein and nucleic acid gels and blots.

myECL Imager

The Thermo Scientific myECL Imager is a stylish, powerful and easy-to-use blot and gel documentation instrument for sensitive, multimode image capture and analysis via an intuitive touchscreen interface and advanced integrated software.

The myECL Imager revolutionizes image acquisition of protein and nucleic acid blots and gels detected via chemiluminescent, colorimetric or UV light-activated fluorescent substrates or stains. The compact benchtop instrument uses UV and visible light transillumination, specialized filters and advanced CCD camera technology to capture images with high sensitivity and dynamic range. The large, 10.4-inch touchscreen and on-board computer provide an elegant user-interface to program acquisition settings and manage (store and share) image files. Also included with the instrument is a five-computer license for the Thermo Scientific myImageAnalysis Software, a complete and powerful analysis tool.

Videos that demonstrate capturing images using the Chemi, UV and Vis modes.

 

Highlights:

  • One-touch image acquisition – press any one of several optimized presets in each mode and the imager does all the rest; no focusing or camera settings need to be adjusted
  • Multi-exposure feature – automatically capture a series of images using up to five different preset or user-defined exposure times
  • Automatic visible image capture – system automatically takes a corresponding visible image with every chemiluminescent image exposure; allows overlay alignment with prestained MW markers
  • Remote Tech Support access – share your myECL Imager screen in a live session with Technical Support to receive immediate help while using the instrument
  • Live camera setting – in any mode see a live view of the illuminated platform on the display screen while the door is open so you can place and center the sample
  • Shoot-and-review convenience – imager keeps the last five captured images immediately available in on-screen tabs so you can quickly review, compare, choose and make adjustments to results
  • Interactive Chemi – automatically calculates the exposure time of a Western blot with maximum dynamic range and minimal pixel saturation from a short, 15-second exposure image
  • File manager – easily copies, deletes, exports and edits image information of one or more image files in multiple gallery folders
  • Create dark and bias images – creates new dark and bias master files to compensate for noise coming from the CCD camera during image acquisition
  • Adjust image – adjust the black, white and gamma levels of acquired images to increase sample visibility
  • Intensity display – select a point of interest on the acquired image to view the pixel intensity and pixel coordinates of the corresponding region

Includes:

The myECL Imager ships equipped with 306nm bulbs in the UV transilluminator and the Orange Filter installed in Filter Position 2 (necessary for ethidium bromide and SYPRO* Orange stains). Also included are the following accessories: Chemiluminescence Exposure Screen, White Light Conversion Screen, UV Exposure Screen, Imaging Reference Target, Touchscreen Stylus with Holder, 2GB USB Flash Drive, User Manuals and Quick Start Guides. Each imager purchase also includes a five-computer network license for the myImageAnalysis Software. The instrument ships with a North American power cord; also available are country-specific power cords.

Applications: Image acquisition and analysis for...

  • Chemiluminescent Western blots (ECL substrates)
  • Stained protein gels (coomassie, silver, UV light-excited fluorescent stains)
  • Chemiluminescent nucleic acid blots (e.g., EMSA and Southern blotting using biotin probes, streptavidin-HRP and ECL substrates)
  • Stained nucleic acid gels (ethidium bromide and other UV light-excited fluorescent stains)
Hardware Specifications.
Parameter Detail
CCD camera 16-bit, 4.2 megapixel; thermoelectrically regulated at -25°C (±0.1)
Lens 50mm, ƒ/0.95
Array size (pixels) 2048 x 2048
Pixel size 7.4 x 7.4µm
Field of view 15.0cm x 15.0cm
Dynamic range >4.0 orders of magnitude
Binning modes 1 x 1, 2 x 2, 3 x 3 (default), 4 x 4, 8 x 8
Image capture modes Chemiluminescence, UV transilluminator, epi-white light
Image exposure modes Automatic or manual
Image file format TIFF (16-bit grayscale)
Excitation source 306nm UV transilluminator; Epi-white light
Filter wheel Motorized, 4 position
Computer Internal with 250 GB hard drive†
Touchscreen display 10.4-inch LCD
Ports 3 USB, 1 network
Instrument size, L x W x H 56cm x 31cm x 53cm (22in x 12.2in x 21in)
Weight 29.5kg (65lbs)
†Approximately 200 GB are available for storage of acquired images, providing storage for more than 200,000 image files captured using the default image acquisition setting (3 x 3 binning).

Product Details:

The powerful and easy-to-use myECL Imager delivers a complete set of high sensitivity Western blot and gel documentation tools through a streamlined and intuitive touchscreen interface, convenient acquisition options, and analysis software supplied with the instrument. The myECL Imager incorporates advanced CCD technology that results in greater than two times the sensitivity of X-ray film and 10 times the dynamic range. The imager can be used in chemiluminescent, ultraviolet and visible modes to image Western blots, stained nucleic acid gels or stained protein gels.

myECL Imager provides multiple exposure gel and blot image acquisition with one touch.

The instrument is controlled with an easy-to-use touchscreen interface that simplifies image acquisition. Researchers can set custom exposure ranges with up to five different exposure times or use preset versions. Images are stored to an internal drive in a nonproprietary data format for easy sharing. The file management system has sort and search functionalities to find locally saved images. Files are easily transferred to any computer using one of three USB ports and the included 2GB Flash Drive or through the Ethernet connection at the back of the instrument.

Analyze images by adjusting display settings (saturation, invert, contrast) on-screen with the imager to ensure that the captured data are of suitable quality for the intended use. Then, once the original image is transferred to another computer that is running the myImageAnalysis Software, it can be fully analyzed to identify lanes and bands, overlay molecular weight markers, calculate molecular weights, measure densitometry, etc.

The myECL Imager is easier to set up and use than most instruments of its type, allowing researchers to adapt and use the new technology quickly without the need for an engineering visit. The imager eliminates the need for a darkroom, replacing it with a machine that has a small footprint. Unlike film, the myECL Imager does not require the use of silver-containing chemicals that require hazardous materials handling and disposal.

More Product Data

References:

User manual for myECL Imager
View Quick Start Guide.

For complete user manual,
see Instructions (below).

Hommema, E., et al. (2013). Improved Western blot detection using CCD digital imaging: Comparison of cooled-CCD digital imaging versus X-ray film. App. Note 1602635. Thermo Fisher Scientific, Rockford, IL.

Related Resources:

Download free trial of the myImageAnalysis Software

Related Products:

myImageAnalysis Software
Country-specific and Replacement Power Cords
SuperSignal West Dura Chemiluminescent Substrate
Pierce ECL Plus Substrate
Protein Gel Stains
Protein Ladders (MW Markers)


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
62236 myECL Imager
Formulation: Benchtop instrument with accessory kit
Sufficient For: Image capture and analysis of chromogenic, chemiluminescent and UV-excited fluorescent blots and gels

Instrument:
CCD camera: 16-bit, 4.2 MP; thermoelectrically regulated at -25°C
Motorized fixed lens: 50mm, f/0.95
Light source: 306nm Trans-UV, Epi-white
Filter wheel: 4 position, motorized
Orange Filter (565-620nm bandpass)
Integrated computer with 250GB hard drive (approx. 200GB for data storage)
Touchscreen: 10.4 inch LCD
Ports: 3 USB (2.0), 1 ethernet

Accessory Kit:
myECL Imager Manual, 1 CD
myECL Imager Quick Start Guide
Chemiluminescence Exposure Screen, 1 screen
White Light Conversion Screen, 1 screen
UV Exposure Screen, 1 screen
Touch Screen Stylus and Holder, 1 each
Imaging Reference Target, 1 target
Power Cord with C/13 Connector, 1 device
USB Flash Drive, 2GB, 1 device
myImageAnalysis Software, 1 CD (5 licenses)
1 unit Product Instructions for product #62236 myECL Imager $28,000.00

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EW84857 Extended Warranty
Formulation: One year extended warranty for the myECL Imager
Sufficient For: Covers all parts (non-consumable) and labor; includes shipping and handling
1 warranty $1,500.00

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62238 Orange Filter
Formulation: 565-620nm bandpass filter; threaded, 50mm-diameter glass filter
Sufficient For: Replacement for the pre-installed orange filter in the myECL Imager for detection of colorimetric protein stains (silver, coomassie) and wavelengths of common fluorescent nucleic acid gel stains (e.g., ethidium bromide) and protein stains, such as SYPRO* Orange Protein Gel Stain
1 filter $815.00

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62239 Green Filter
Formulation: 510-550nm bandpass filter; threaded, 50mm-diameter glass filter
Sufficient For: Addition of a green filter to one of two open positions in the myECL Imager for detection (emission) wavelengths of common fluorescent nucleic acid stains, such as SYBR* Green Nucleic Acid Gel Stain and SYBR Safe DNA Gel Stain
1 filter $815.00

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62240 Red Filter
Formulation: 600-640nm bandpass filter; threaded, 50mm-diameter glass filter
Sufficient For: Addition of a red filter to one of two open positions in the myECL Imager for detection (emission) wavelengths of common fluorescent protein stains, such as SYPRO* Red Protein Gel Stain, SYPRO Ruby Protein Gel Stain and Deep Purple* Total Protein Stain.
1 filter $815.00

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62241 Blue Filter
Formulation: 445-480nm bandpass filter; threaded, 50mm-diameter glass filter
Sufficient For: Addition of a blue filter to one of two open positions in the myECL Imager for detection of blue-fluorescent dyes and stains
1 filter $815.00

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62242 Chemiluminescence Exposure Screen
Formulation: Black plastic sample screen to cover the transilluminator; 24.5cm x 19.5cm x 3.2mm
Sufficient For: Replacement of myECL Imager accessory; used on the transilluminator for acquiring chemiluminescent images
1 screen $409.00

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62243 White Light Conversion Screen
Formulation: White glass sample screen to cover the transilluminator; 24.5cm x 19.5cm x 3.2mm
Sufficient For: Replacement of myECL Imager accessory; used on the transilluminator for acquiring images in the epi-white (visible mode)
1 screen $409.00

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62244 UV Exposure Screen
Formulation: Clear plastic sample screen to cover the transilluminator; 24.5cm x 19.5cm x 3.2mm
Sufficient For: Replacement of myECL Imager accessory; used on the transilluminator for acquiring images in the UV mode
1 screen $409.00

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62267 Imaging Reference Target
Formulation: Plastic imaging target with printed design; 7in x 6.5in
Sufficient For: Replacement of myECL Imager accessory; used for focusing in all imaging modes
1 target $101.00

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62255 UV Transilluminator Bulb, 365nm
Formulation: Lamp bulb with ultraviolet emission (365nm)
Sufficient For: Substitution (replacement) of the pre-installed 306nm UV bulbs in the myECL Imager with long-wave 365nm bulbs; All six lamps must be the same bulb type (365nm or 306nm)
1 bulb $46.00

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62258 UV Transilluminator Bulb, 306nm
Formulation: Lamp bulb with ultraviolet emission (306nm)
Sufficient For: Replacement of one of six pre-installed 306nm UV bulbs in the myECL Imager; All six lamps must be the same bulb type (306nm or 365nm)
1 bulb $46.00

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62261 Touchscreen Stylus and Holder
Formulation: Black stylus resembling a pen and round plastic clip to mount on the side of the imager
Sufficient For: Replacement of myECL Imager accessory; for interaction with the imager touchscreen
1 set $21.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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