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MPBH


A long crosslinker for conjugating glycoprotein carbohydrates to sulfhydryl groups.

Pierce MPBH

Thermo Scientific Pierce MPBH is a long, maleimide-and-hydrazide crosslinker for conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).

Highlights:

  • Reactive groups: maleimide and hydrazide
  • Reactive towards: sulfhydryl groups and carbonyl (aldehyde) groups
  • Long (17.9A), sulfhydryl-to-aldehyde crosslinker with an aromatic ring in its spacer arm (noncleavable)
  • Maleimide group reacts with sulfhydryl groups to form stable thioether linkages
  • Hydrazide group conjugates to oxidized sugars of glycoproteins and carbohydrates
  • Use sodium meta-periodate to oxidize glycosylation (e.g., sialic acid) to reactive aldehyde groups
  • Use with EDC to conjugate primary amine of hydrazide group to carboxyl groups

Product Details:

4-(4-N-Maleimidophenyl)butyric acid hydrazide-HCl; MPBH
 
Chemical structure of MPBH crosslinking reagent.

References:

  1. Chamow, S.M., Kogan, T.P., Peers, D.H., Hastings, R.C., Byrn, R.A. and Askenaszi, A. (1992). Conjugation of soluble CD4 without loss of biological activity via a novel carbohydrate-directed crosslinking reagent. J. Biol. Chem. 267(22), 15916-15922.

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Resources:

Overview of crosslinking
Chemistry of crosslinking
Protein glycosylation technical guide

Related Products:

Crosslinkers at a glance (selection guide)


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
22305 MPBH
Formulation: 4-(4-N-Maleimidophenyl)butyric acid hydrazide-HCl; powder solid
Sufficient For: 141mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use
50mg Product Instructions for product #22305 MPBH MSDS for product #22305 MPBH Certificate of Analysis for product #22305 MPBH $208.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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