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A long crosslinker for conjugating glycoprotein carbohydrates to sulfhydryl groups.

Pierce MPBH

Thermo Scientific Pierce MPBH is a long, maleimide-and-hydrazide crosslinker for conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).


  • Reactive groups: maleimide and hydrazide
  • Reactive towards: sulfhydryl groups and carbonyl (aldehyde) groups
  • Long (17.9A), sulfhydryl-to-aldehyde crosslinker with an aromatic ring in its spacer arm (noncleavable)
  • Maleimide group reacts with sulfhydryl groups to form stable thioether linkages
  • Hydrazide group conjugates to oxidized sugars of glycoproteins and carbohydrates
  • Use sodium meta-periodate to oxidize glycosylation (e.g., sialic acid) to reactive aldehyde groups
  • Use with EDC to conjugate primary amine of hydrazide group to carboxyl groups

Product Details:

4-(4-N-Maleimidophenyl)butyric acid hydrazide-HCl; MPBH
Chemical structure of MPBH crosslinking reagent.


  1. Chamow, S.M., Kogan, T.P., Peers, D.H., Hastings, R.C., Byrn, R.A. and Askenaszi, A. (1992). Conjugation of soluble CD4 without loss of biological activity via a novel carbohydrate-directed crosslinking reagent. J. Biol. Chem. 267(22), 15916-15922.

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Resources:

Overview of crosslinking
Chemistry of crosslinking
Protein glycosylation technical guide

Related Products:

Crosslinkers at a glance (selection guide)

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
22305 MPBH
Formulation: 4-(4-N-Maleimidophenyl)butyric acid hydrazide-HCl; powder solid
Sufficient For: 141mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use
50mg Product Instructions for product #22305 MPBH MSDS for product #22305 MPBH Certificate of Analysis for product #22305 MPBH $300.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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