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Mouse TNF alpha ELISA Kits


Microplate kits and reagent sets for sandwich ELISA of mouse TNF alpha.

Thermo Scientific Mouse TNF alpha ELISA Kits

Assay TNFα (mouse tumor necrosis factor alpha) levels in mouse serum and culture supernatant samples with our Thermo Scientific Mouse TNF alpha ELISA Kits.

These mouse TNF alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of tumor necrosis factor alpha (TNF-alpha, TNFα) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The antibody-pair kit consists of the matched TNFα antibody pairs and TNFα standard only. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

  • Target: natural and recombinant mouse TNFα (mouse tumor necrosis factor alpha)
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 50 to 2450pg/mL
  • Sensitivity: < 9pg/mL
  • Precision: intra-assay CV < 10%; inter-assay CV < 10%
  • Sample Types: cell culture supernatant, serum
  • Total Assay Time: 3 hours
  • Sample Size: 50μL
  • Specificity: no significant cross-reaction with mouse IL-2, IL-3, IL-4, IL-5, IL-6, GM-CSF, IFNγ or human TNFα. This ELISA has some cross-reactivity with rabbit and cynomologous monkey TNFα in culture supernatants.

Note: Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the antibody-pair kit.

Video of the Thermo Scientific TNF alpha ELISA Kit

Includes:

Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Antibody Pair Kit (previously called a "MiniKit") contains only matched antibodies and standard (no plates or detection reagents).

» Compare ELISA Kit formats »

Details:

Mouse TNF alpha ELISA standard curve
Mouse TNF alpha ELISA standard curve. An example standard curve for the Thermo Scientific Mouse TNF alpha ELISA Kit (Part No. EMTNFA), with error bars based on six replicates for each tumor necrosis factor alpha standard concentration. Also see standard curve for the TNFα Antibody Pair Kit.

TNFα, also called cachectin, macrophage cytotoxin, necrosin, etc., is produced primarily by macrophages in response to bacteria, viruses, mycoplasma, immune complexes, other cytokines, tumor cells, complement and phorbol esters among others. It is a 157 amino acid long polypeptide with an apparent MW of 17 kDa under denaturing conditions but exists as a 45-55 kDa trimer naturally.

In vivo, TNFα has anti-tumor activities against some solid tumors, induces tumorigenesis, metastisis; acts in immunomodulation; induces septic shock, fever; plays a role in multiple sclerosis; and is involved in autoimmunity and infectious disease. TNFα alone and in combination with other cytokines and chemical agents has been used as an anti-tumor agent. Patients with melanoma and other solid tumors, administered high doses of TNFα, have realized a cure rate of up to 90% albeit with some major and minor side effects.

References:

Related Resources:

Related Products:

Notice:

This product is for In Vitro experimental use only. Not for resale without express authorization.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
EMTNFA Mouse TNF alpha ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-Mouse TNF alpha Precoated 96-well Strip Plate, 1 each
Lyophilized Recombinant Mouse TNF alpha Standard, 2 vials
Standard Diluent, 12mL
Biotinylated Antibody Reagent, 8mL
30X Wash Buffer, 50mL
Streptavidin-HRP Concentrate, 100μL
HRP Dilution Buffer, 14mL
TMB Substrate Solution, 13mL
Stop Solution, 13mL
Adhesive Plate Covers, 6 each
1-plate kit Product Instructions for product #EMTNFA Mouse TNF alpha ELISA Kit MSDS for product #EMTNFA Mouse TNF alpha ELISA Kit $495.00

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EMTNFA2 Mouse TNF alpha ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 2 x 96 microplate wells

Kit Contents:
Anti-Mouse TNF alpha Precoated 96-well Strip Plate, 2 each
Lyophilized Recombinant Mouse TNF alpha Standard, 4 vials
Standard Diluent, 2 x 12mL
Biotinylated Antibody Reagent, 2 x 8mL
30X Wash Buffer, 2 x 50mL
Streptavidin-HRP Concentrate, 2 x 100μL
HRP Dilution Buffer, 2 x 14mL
TMB Substrate Solution, 2 x 13mL
Stop Solution, 2 x 13mL
Adhesive Plate Covers, 12 each
2-plate kit Product Instructions for product #EMTNFA2 Mouse TNF alpha ELISA Kit MSDS for product #EMTNFA2 Mouse TNF alpha ELISA Kit $750.00

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EMTNFA5 Mouse TNF alpha ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 5 x 96 microplate wells

Kit Contents:
Anti-Mouse TNF alpha Precoated 96-well Strip Plate, 5 each
Lyophilized Recombinant Mouse TNF alpha Standard, 5 vials
Standard Diluent, 55mL
Biotinylated Antibody Reagent, 30mL
30X Wash Buffer, 200mL
Streptavidin-HRP Concentrate, 250μL
HRP Dilution Buffer, 70mL
TMB Substrate Solution, 5 x 13mL
Stop Solution, 55mL
Adhesive Plate Covers, 30 each
5-plate kit Product Instructions for product #EMTNFA5 Mouse TNF alpha ELISA Kit MSDS for product #EMTNFA5 Mouse TNF alpha ELISA Kit $1,650.00

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SMTNFA Mouse TNF alpha Standard for ELISA
Formulation: Calibrated to NIBSC reference lot 88/532
Sufficient For: ELISA standard curve with replicates
1 vial MSDS for product #SMTNFA Mouse TNF alpha Standard for ELISA $110.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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