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Mouse Soluble ICAM-1 (CD54) ELISA Kits


Microplate kits for sandwich ELISA of mouse intercellular adhesion molecule 1 (ICAM-1, CD54).

Thermo Scientific Mouse ICAM-1 CD54 ELISA Kits

Assay sICAM-1 (mouse soluble ICAM-1, also called CD54) levels in mouse serum, plasma (EDTA) and culture supernatant samples with our Thermo Scientific Mouse Soluble ICAM (CD54) ELISA Kits.

These mouse sICAM-1 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of soluble ICAM-1 (intercellular adhesion molecule 1, also called CD54) in samples at 50μL per well. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

  • Target: mouse soluble ICAM-1 (intercellular adhesion molecule 1, also called CD54)
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 25.6 to 1000ng/mL
  • Sensitivity: < 5ng/mL
  • Precision: intra-assay CV < 10%; inter-assay CV < 10%
  • Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 3.5 hours
  • Sample Size: 50μL of 100-fold diluted samples per well
  • Specificity: no significant cross-reaction with rat or human sICAM-1

Includes:

Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Details:

Mouse ICAM-1 (CD54) ELISA standard curve
Mouse ICAM-1 ELISA standard curve. An example standard curve for the Thermo Scientific Mouse ICAM-1 (CD54) ELISA Kit (Part No. EMICAM1), with error bars based on twelve replicates for each ICAM-1 (CD54) standard concentration.

References:

Related Resources:

Related Products:

Notice:

This product is for In Vitro experimental use only. Not for resale without express authorization.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
EMICAM1 Mouse Soluble ICAM-1 (CD54) ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-Mouse sICAM-1 Precoated 96-well Strip Plate, 1 each
Lyophilized Mouse sICAM-1 Standard, 2 vials
Standard Diluent, 12mL
Plate Reagent, 8mL
30X Wash Buffer, 50mL
Anti-mouse sICAM-1 HRP-Conjugate, 11mL
TMB Substrate Solution, 13mL
Stop Solution, 13mL
Adhesive Plate Covers, 6 each
1-plate kit Product Instructions for product #EMICAM1 Mouse Soluble ICAM-1 (CD54) ELISA Kit MSDS for product #EMICAM1 Mouse Soluble ICAM-1 (CD54) ELISA Kit $399.00

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EMICAM12 Mouse Soluble ICAM-1 (CD54) ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 2 x 96 microplate wells

Kit Contents:
Anti-Mouse sICAM-1 Precoated 96-well Strip Plate, 2 each
Lyophilized Mouse sICAM-1 Standard, 4 vials
Standard Diluent, 2 x 12mL
Plate Reagent, 2 x 8mL
30X Wash Buffer, 2 x 50mL
Anti-mouse sICAM-1 HRP-Conjugate, 2 x 11mL
TMB Substrate Solution, 2 x 13mL
Stop Solution, 2 x 13mL
Adhesive Plate Covers, 12 each
2-plate kit Product Instructions for product #EMICAM12 Mouse Soluble ICAM-1 (CD54) ELISA Kit MSDS for product #EMICAM12 Mouse Soluble ICAM-1 (CD54) ELISA Kit $650.00

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EMICAM15 Mouse Soluble ICAM-1 (CD54) ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 5 x 96 microplate wells

Kit Contents:
Anti-Mouse sICAM-1 Precoated 96-well Strip Plate, 5 each
Lyophilized Mouse sICAM-1 Standard, 10 vials
Standard Diluent, 2 x 12mL
Plate Reagent, 5 x 8mL
30X Wash Buffer, 5 x 50mL
Anti-mouse sICAM-1 HRP-Conjugate, 5 x 11mL
TMB Substrate Solution, 5 x 13mL
Stop Solution, 5 x 13mL
Adhesive Plate Covers, 30 each
5-plate kit Product Instructions for product #EMICAM15 Mouse Soluble ICAM-1 (CD54) ELISA Kit MSDS for product #EMICAM15 Mouse Soluble ICAM-1 (CD54) ELISA Kit $1,500.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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