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Mouse MCP-1 ELISA Kits


Microplate and reagent kits for sandwich ELISA of mouse MCP-1.

Thermo Scientific Mouse MCP-1 ELISA Kits

Assay MCP-1 (mouse monocyte chemoattractant protein-1) levels in mouse serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Mouse MCP-1 ELISA Kits.

These mouse MCP-1 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of monocyte chemoattractant protein-1 (MCP-1) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

  • Target: natural and recombinant mouse MCP-1 (monocyte chemoattractant protein-1)
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 16 to 1000pg/mL
  • Sensitivity: < 4pg/mL
  • Precision: intra-assay CV < 10%; inter-assay CV < 10%
  • Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 3 hours
  • Sample Size: 50μL
  • Specificity: no significant cross-reaction with mouse G-CSF, GM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-18, Eotaxin, MIP-1α, RANTES or TNFα, or human or rat MCP-1.

Note: Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit.

Video of the Thermo Scientific MCP-1 ELISA Kit

Includes:

Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates.

» Compare ELISA Kit formats »

Details:

Mouse MCP-1 ELISA standard curve
Mouse MCP-1 ELISA standard curve. An example standard curve for the Thermo Scientific Mouse MCP-1 ELISA Kit (Part No. EH2MCP1), with error bars based on four replicates for each MCP-1 standard concentration. Also see standard curve for the MCP-1 Reagent Kit.

References:

Related Resources:

Related Products:

Notice:

This product is for In Vitro experimental use only. Not for resale without express authorization.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
EMMCP1 Mouse MCP-1 ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-Mouse MCP-1 Precoated 96-well Strip Plate, 1 each
Lyophilized Recombinant Mouse MCP-1 Standard, 2 vials
Sample Diluent, 12mL
Biotinylated Antibody Reagent, 12mL
30X Wash Buffer, 50mL
Streptavidin-HRP Concentrate, 75μL
HRP Dilution Buffer, 13mL
TMB Substrate Solution, 12mL
Stop Solution, 13mL
Adhesive Plate Covers, 6 each
1-plate kit Product Instructions for product #EMMCP1 Mouse MCP-1 ELISA Kit MSDS for product #EMMCP1 Mouse MCP-1 ELISA Kit $399.00

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EMMCP12 Mouse MCP-1 ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 2 x 96 microplate wells

Kit Contents:
Anti-Mouse MCP-1 Precoated 96-well Strip Plate, 2 each
Lyophilized Recombinant Mouse MCP-1 Standard, 4 vials
Sample Diluent, 2 x 12mL
Biotinylated Antibody Reagent, 2 x 12mL
30X Wash Buffer, 2 x 50mL
Streptavidin-HRP Concentrate, 2 x 75μL
HRP Dilution Buffer, 2 x 13mL
TMB Substrate Solution, 2 x 12mL
Stop Solution, 2 x 13mL
Adhesive Plate Covers, 12 each
2-plate kit Product Instructions for product #EMMCP12 Mouse MCP-1 ELISA Kit MSDS for product #EMMCP12 Mouse MCP-1 ELISA Kit $650.00

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EMMCP15 Mouse MCP-1 ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 5 x 96 microplate wells

Kit Contents:
Anti-Mouse MCP-1 Precoated 96-well Strip Plate, 5 each
Lyophilized Recombinant Mouse MCP-1 Standard, 10 vials
Sample Diluent, 5 x 12mL
Biotinylated Antibody Reagent, 5 x 12mL
30X Wash Buffer, 5 x 50mL
Streptavidin-HRP Concentrate, 5 x 75┬ÁL
HRP Dilution Buffer, 5 x 13mL
TMB Substrate Solution, 5 x 12mL
Stop Solution, 5 x 13mL
Adhesive Plate Covers, 30 each
5-plate kit Product Instructions for product #EMMCP15 Mouse MCP-1 ELISA Kit MSDS for product #EMMCP15 Mouse MCP-1 ELISA Kit $1,500.00

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ESS0004 Mouse MCP-1 ELISA Reagent Kit
Formulation: Antibodies and complete reagent set for ELISA
Sufficient For: 5 x 96 microplate wells

Kit Contents:
MCP-1 Coating Antibody, 1 vial
MCP-1 Detection Antibody, 1 vial
Human MCP-1 Standard, 5 vials
Streptavidin-HRP Conjugate, 0.25mL
TMB Substrate Solution, 55mL
Stop Solution, 55mL
5-plate kit Product Instructions for product #ESS0004 Mouse MCP-1 ELISA Reagent Kit MSDS for product #ESS0004 Mouse MCP-1 ELISA Reagent Kit $375.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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