Update profile
    Change password
    Sign out

Mouse IL-6 ELISA Kits


Microplate kits and reagent sets for sandwich ELISA of mouse interleukin-6 (IL-6).

Thermo Scientific Mouse IL-6 ELISA Kits

Assay IL-6 (mouse interleukin-6) levels in mouse serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Mouse IL-6 ELISA Kits.

These mouse IL-6 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-6 (IL-6) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. The antibody-pair kit consists of the matched IL-6 antibody pairs and IL-6 standard only. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

  • Target: natural and recombinant mouse interleukin-6 (IL-6)
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 51 to 2000pg/mL
  • Sensitivity: < 7pg/mL
  • Precision: intra-assay CV < 10%; inter-assay CV < 10%
  • Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 4 hours
  • Sample Size: 50μL
  • Specificity: no significant cross-reaction with mouse GM-CSF, IFNγ, IL-1α, IL-2, IL-3, IL-4, IL-5, IL-7, IL-9, IL-10, IL-12, or human IL-6. This kit has been shown to have a high level of cross-reactivity with recombinant rat IL-6.

Note: Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the antibody-pair kit.

Video of the Thermo Scientific IL-6 ELISA Kit

Includes:

Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Antibody Pair Kit (previously called a "MiniKit") contains only matched antibodies and standard (no plates or detection reagents).

» Compare ELISA Kit formats »

Details:

Mouse IL-6 ELISA standard curve
Mouse IL-6 ELISA standard curve. An example standard curve for the Thermo Scientific Mouse IL-6 ELISA Kit (Part No. EM2IL6), with error bars based on four replicates for each interleukin-6 standard concentration. Also see standard curves for the IL-6 Antibody Pair Kit.

References:

Related Resources:

Related Products:

Notice:

This product is for In Vitro experimental use only. Not for resale without express authorization.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
EM2IL6 Mouse IL-6 ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-Mouse IL-6 Precoated 96-well Strip Plate, 1 each
Lyophilized Recombinant Mouse IL-6 Standard, 2 vials
Standard Diluent, 25mL
Biotinylated Antibody Reagent, 8mL
30X Wash Buffer, 50mL
Streptavidin-HRP Concentrate, 100μL
HRP Dilution Buffer, 14mL
TMB Substrate Solution, 13mL
Stop Solution, 13mL
Adhesive Plate Covers, 6 each
1-plate kit Product Instructions for product #EM2IL6 Mouse IL-6 ELISA Kit MSDS for product #EM2IL6 Mouse IL-6 ELISA Kit $495.00

Add to cart
EM2IL62 Mouse IL-6 ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 2 x 96 microplate wells

Kit Contents:
Anti-Mouse IL-6 Precoated 96-well Strip Plate, 2 each
Lyophilized Recombinant Mouse IL-6 Standard, 4 vials
Standard Diluent, 25mL
Biotinylated Antibody Reagent, 2 x 8mL
30X Wash Buffer, 2 x 50mL
Streptavidin-HRP Concentrate, 100μL
HRP Dilution Buffer, 26mL
TMB Substrate Solution, 2 x 13mL
Stop Solution, 2 x 13mL
Adhesive Plate Covers, 12 each
2-plate kit Product Instructions for product #EM2IL62 Mouse IL-6 ELISA Kit MSDS for product #EM2IL62 Mouse IL-6 ELISA Kit $740.00

Add to cart
EM2IL65 Mouse IL-6 ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 5 x 96 microplate wells

Kit Contents:
Anti-Mouse IL-6 Precoated 96-well Strip Plate, 5 each
Lyophilized Recombinant Mouse IL-6 Standard, 5 vials
Standard Diluent, 55mL
Biotinylated Antibody Reagent, 30mL
30X Wash Buffer, 200mL
Streptavidin-HRP Concentrate, 250μL
HRP Dilution Buffer, 70mL
TMB Substrate Solution, 5 x 13mL
Stop Solution, 55mL
Adhesive Plate Covers, 30 each
5-plate kit Product Instructions for product #EM2IL65 Mouse IL-6 ELISA Kit MSDS for product #EM2IL65 Mouse IL-6 ELISA Kit $1,600.00

Add to cart
KMIL6 Mouse IL-6 ELISA Antibody Pair Kit
Formulation: Matched antibodies and standard for ELISA
Sufficient For: 40 x 96 microplate wells

Kit Contents:
IL-6 Coating Antibody, 1 vial
IL-6 Biotinylated Detection Antibody, 1 vial
Mouse IL-6 Standard, 1 vial
40-plate kit Product Instructions for product #KMIL6 Mouse IL-6 ELISA Antibody Pair Kit MSDS for product #KMIL6 Mouse IL-6 ELISA Antibody Pair Kit $999.00

Add to cart
SMIL6 Mouse IL-6 Standard for ELISA
Formulation: Calibrated to NIBSC reference lot 93/730
Sufficient For: ELISA standard curve with replicates
1 vial MSDS for product #SMIL6 Mouse IL-6 Standard for ELISA $110.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

Share This Page
 
 

Follow Us
  Email Sign-up  Email Announcements

 



Antibodies  |   Molecular Biology   |   Cell Biology   |  Thermo Scientific  |   * Trademarks  |   Privacy Statement
© 2014 Thermo Fisher Scientific Inc.   |   3747 N Meridian Rd, Rockford, IL USA 61101   |   1-800-874-3723  or  815-968-0747