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Mouse IL-5 ELISA Kits


Microplate kits and reagent sets for sandwich ELISA of mouse interleukin-5 (IL-5).

Thermo Scientific Mouse IL-5 ELISA Kits

Assay IL-5 (mouse interleukin-5) levels in mouse serum and culture supernatant samples with our Thermo Scientific Mouse IL-5 ELISA Kits.

These mouse IL-5 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-5 (IL-5) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. The antibody-pair kit consists of the matched IL-5 antibody pairs and IL-5 standard only. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

  • Target: natural and recombinant mouse interleukin-5 (IL-5)
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 20 to 320pg/mL
  • Sensitivity: < 5pg/mL
  • Precision: intra-assay CV < 10%; inter-assay CV < 10%
  • Sample Types: cell culture supernatant, serum
  • Total Assay Time: 3.5 hours
  • Sample Size: 50μL
  • Specificity: no significant cross-reaction with with mouse IL-2, IL-3, IL-4, IL-6, IFNγ, GM-CSF, TNFα, rat IL-5, rabbit IL-5 or human IL-5.

Note: Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the antibody-pair kit.

Video of the Thermo Scientific IL-5 ELISA Kit

Includes:

Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Antibody Pair Kit (previously called a "MiniKit") contains only matched antibodies and standard (no plates or detection reagents).

» Compare ELISA Kit formats »

Details:

Mouse IL-5 ELISA standard curve
Mouse IL-5 ELISA standard curve. An example standard curve for the Thermo Scientific Mouse IL-5 ELISA Kit (Part No. EMIL5), with error bars based on twelve replicates for each interleukin-5 standard concentration. Also see standard curves for the IL-5 Antibody Pair Kit.

References:

Related Resources:

Related Products:

Notice:

This product is for In Vitro experimental use only. Not for resale without express authorization.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
EMIL5 Mouse IL-5 ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-Mouse IL-5 Precoated 96-well Strip Plate, 1 each
Lyophilized Recombinant Mouse IL-5 Standard, 2 vials
Standard Diluent, 12mL
Plate Reagent, 8mL
30X Wash Buffer, 50mL
Anti-Mouse IL-5 HRP Conjugate Reagent, 12mL
TMB Substrate Solution, 13mL
Stop Solution, 13mL
Adhesive Plate Covers, 6 each
1-plate kit Product Instructions for product #EMIL5 Mouse IL-5 ELISA Kit MSDS for product #EMIL5 Mouse IL-5 ELISA Kit $399.00

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EMIL52 Mouse IL-5 ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 2 x 96 microplate wells

Kit Contents:
Anti-Mouse IL-5 Precoated 96-well Strip Plate, 2 each
Lyophilized Recombinant Mouse IL-5 Standard, 4 vials
Standard Diluent, 2 x 12mL
Plate Reagent, 2 x 8mL
30X Wash Buffer, 2 x 50mL
Anti-Mouse IL-5 HRP Conjugate Reagent, 2 x 12mL
TMB Substrate Solution, 2 x 13mL
Stop Solution, 2 x 13mL
Adhesive Plate Covers, 12 each
2-plate kit Product Instructions for product #EMIL52 Mouse IL-5 ELISA Kit MSDS for product #EMIL52 Mouse IL-5 ELISA Kit $650.00

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EMIL55 Mouse IL-5 ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 5 x 96 microplate wells

Kit Contents:
Anti-Mouse IL-5 Precoated 96-well Plate Strip, 5 each
Lyophilized Recombinant Mouse IL-5 Standard, 10 vials
Standard Diluent, 5 x 12mL
Plate Reagent, 5 x 8mL
30X Wash Buffer, 5 x 50mL
Anti-Mouse IL-5 HRP Conjugate Reagent, 5 x 12mL
TMB Substrate Solution, 5 x 13mL
Stop Solution, 5 x 13mL
Adhesive Plate Covers, 30 each
5-plate kit Product Instructions for product #EMIL55 Mouse IL-5 ELISA Kit MSDS for product #EMIL55 Mouse IL-5 ELISA Kit $1,500.00

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KMIL5 Mouse IL-5 ELISA Antibody Pair Kit
Formulation: Matched antibodies and standard for ELISA
Sufficient For: 40 x 96 microplate wells

Kit Contents:
IL-5 Coating Antibody, 1 vial
IL-5 Biotinylated Detection Antibody, 1 vial
Mouse IL-5 Standard, 1 vial
40-plate kit Product Instructions for product #KMIL5 Mouse IL-5 ELISA Antibody Pair Kit MSDS for product #KMIL5 Mouse IL-5 ELISA Antibody Pair Kit $999.00

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SMIL5 Mouse IL-5 Standard for ELISA
Formulation: Calibrated to Thermo Scientific Internal Reference Standard
Sufficient For: ELISA standard curve with replicates
1 vial MSDS for product #SMIL5 Mouse IL-5 Standard for ELISA $110.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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