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Mouse IL-12 (p70) ELISA Kits


Microplate kits for sandwich ELISA of biologically active mouse interleukin-12 (IL-12 p70).

Thermo Scientific Mouse IL-12 ELISA Kits

Assay IL-12 p70 (active mouse interleukin-12, p70) levels in mouse serum and culture supernatant samples with our Thermo Scientific Mouse IL-12 (p70) ELISA Kits.

These mouse IL-12 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of biologically active interleukin-12 (IL-12 p70) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

  • Target: biologically active (p70) natural and recombinant mouse interleukin-12 (IL-12, p70)
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 12.3 to 1000pg/mL
  • Sensitivity: < 5pg/mL
  • Precision: intra-assay CV < 10%; inter-assay CV < 10%
  • Sample Types: cell culture supernatant, serum
  • Total Assay Time: 3 hours
  • Sample Size: 50μL
  • Specificity: no significant cross-reaction with mouse IL-1α, IL-1β, IL-3, IL-4, IL-5, IL-6, IL-7, TNFα, IFNγ, GM-CSF, or human IL-12 (p70). Recombinant mouse IL-12 (p40) and human IL-12 (p40) do not cross-react or interfere with this assay.
Video of the Thermo Scientific IL-12 ELISA Kit

Includes:

Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Details:

Mouse IL-12 p70 ELISA standard curve
Mouse active IL-12 ELISA standard curve. An example standard curve for the Thermo Scientific Mouse IL-12 p70 ELISA Kit (Part No. EMIL12), with error bars based on five replicates for each biologically active interleukin-12 p70 standard concentration.

References:

Related Resources:

Related Products:

Notice:

This product is for In Vitro experimental use only. Not for resale without express authorization.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
EMIL12 Mouse IL-12 (p70) ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-Mouse IL-12 p70 Precoated 96-well Strip Plate, 1 each
Lyophilized Recombinant Mouse IL-12 p70 Standard, 2 vials
Standard Diluent, 12mL
Biotinylated Antibody Concentrate, 125μL
Biotinylated Antibody Diluent, 10mL
30X Wash Buffer, 50mL
Streptavidin-HRP Concentrate, 75μL
HRP Dilution Buffer, 14mL
TMB Substrate Solution, 13mL
Stop Solution, 13mL
Adhesive Plate Covers, 6 each
1-plate kit Product Instructions for product #EMIL12 Mouse IL-12 (p70) ELISA Kit MSDS for product #EMIL12 Mouse IL-12 (p70) ELISA Kit $399.00

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EMIL122 Mouse IL-12 (p70) ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 2 x 96 microplate wells

Kit Contents:
Anti-Mouse IL-12 p70 Precoated 96-well Strip Plate, 2 each
Lyophilized Recombinant Mouse IL-12 p70 Standard, 4 vials
Standard Diluent, 2 x 12mL
Biotinylated Antibody Concentrate, 2 x 125μL
Biotinylated Antibody Diluent, 2 x 10mL
30X Wash Buffer, 2 x 50mL
Streptavidin-HRP Concentrate, 2 x 75μL
HRP Dilution Buffer, 2 x 14mL
TMB Substrate Solution, 2 x 13mL
Stop Solution, 2 x 13mL
Adhesive Plate Covers, 12 each
2-plate kit Product Instructions for product #EMIL122 Mouse IL-12 (p70) ELISA Kit MSDS for product #EMIL122 Mouse IL-12 (p70) ELISA Kit $650.00

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EMIL125 Mouse IL-12 (p70) ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 5 x 96 microplate wells

Kit Contents:
Anti-Mouse IL-12 p70 Precoated 96-well Strip Plate, 5 each
Lyophilized Recombinant Mouse IL-12 p70 Standard, 5 vials
Standard Diluent, 55mL
Biotinylated Antibody Concentrate, 500μL
Biotinylated Antibody Diluent, 45mL
30X Wash Buffer, 200mL
Streptavidin-HRP Concentrate, 250μL
HRP Dilution Buffer, 70mL
TMB Substrate Solution, 5 x 13mL
Stop Solution, 55mL
Adhesive Plate Covers, 30 each
5-plate kit Product Instructions for product #EMIL125 Mouse IL-12 (p70) ELISA Kit MSDS for product #EMIL125 Mouse IL-12 (p70) ELISA Kit $1,500.00

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SMIL12 Mouse IL-12 (p70) Standard for ELISA
Formulation: Calibrated to Thermo Scientific Internal Reference Standard
Sufficient For: ELISA standard curve with replicates
1 vial MSDS for product #SMIL12 Mouse IL-12 (p70) Standard for ELISA $110.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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