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Mouse IL-1 beta ELISA Kits


Microplate kits for sandwich ELISA of mouse interleukin-1 beta (IL-1b).

Thermo Scientific Mouse IL-1 beta ELISA Kits

Assay IL-1β (mouse interleukin-1 beta) levels in mouse serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Mouse IL-1 beta ELISA Kits.

These mouse IL-1 beta ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-1 beta (IL-1β) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

  • Target: natural and recombinant mouse interleukin-1 beta (IL-1β)
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 31.2 to 2000pg/mL
  • Sensitivity: < 3pg/mL
  • Precision: intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
  • Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 4 hours
  • Sample Size: 50μL
  • Specificity: no significant cross-reaction with mouse IL-1α, IL-10, IL-2, IL-4, IL-6, TNFα, GM-CSF; human IL-1β, IL-8, IL-1α; or bovine IL-1β. This ELISA does cross-react with rat IL-1β (12%).
Video of the Thermo Scientific IL-1 beta ELISA Kit

Includes:

Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Details:

Mouse IL-1 beta ELISA standard curve
Mouse IL-1β ELISA standard curve. An example standard curve for the Thermo Scientific Mouse IL-1β ELISA Kit (Part No. EM2IL1B), with error bars based on twelve replicates for each interleukin-1 beta standard concentration.

References:

Related Resources:

Related Products:

Notice:

This product is for In Vitro experimental use only. Not for resale without express authorization.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
EM2IL1B Mouse IL-1 beta ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-Mouse IL-1 beta Precoated 96-well Strip Plate, 1 each
Lyophilized Recombinant Mouse IL-1 beta Standard, 2 vials
Standard Diluent, 12mL
Biotinylated Antibody Reagent, 12mL
20X Wash Buffer, 55mL
Streptavidin-HRP Concentrate, 75μL
HRP Dilution Buffer, 13mL
TMB Substrate Solution, 12mL
Stop Solution, 6mL
Adhesive Plate Covers, 6 each
1-plate kit Product Instructions for product #EM2IL1B Mouse IL-1 beta ELISA Kit MSDS for product #EM2IL1B Mouse IL-1 beta ELISA Kit $495.00

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EM2IL1B2 Mouse IL-1 beta ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 2 x 96 microplate wells

Kit Contents:
Anti-Mouse IL-1 beta Precoated 96-well Strip Plate, 2 each
Lyophilized Recombinant Mouse IL-1 beta Standard, 4 vials
Standard Diluent, 2 x 12mL
Biotinylated Antibody Reagent, 2 x 12mL
20X Wash Buffer, 2 x 55mL
Streptavidin-HRP Concentrate, 2 x 75μL
HRP Dilution Buffer
TMB Substrate Solution, 2 x 12mL
Stop Solution, 2 x 6mL
Adhesive Plate Covers, 12 each
2-plate kit Product Instructions for product #EM2IL1B2 Mouse IL-1 beta ELISA Kit MSDS for product #EM2IL1B2 Mouse IL-1 beta ELISA Kit $740.00

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EM2IL1B5 Mouse IL-1 beta ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 5 x 96 microplate wells

Kit Contents:
Anti-Mouse IL-1 beta Precoated 96-well Strip Plate, 5 each
Lyophilized Recombinant Mouse IL-1 beta Standard, 5 vials
Standard Diluent, 55mL
Biotinylated Antibody Reagent, 55mL
20X Wash Buffer, 250mL
Streptavidin-HRP Concentrate, 250μL
HRP Dilution Buffer, 70mL
TMB Substrate Solution, 5 x 12mL
Stop Solution, 30mL
Adhesive Plate Covers, 30 each
5-plate kit Product Instructions for product #EM2IL1B5 Mouse IL-1 beta ELISA Kit MSDS for product #EM2IL1B5 Mouse IL-1 beta ELISA Kit $1,600.00

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SMIL1B Mouse IL-1 beta Standard for ELISA
Formulation: Calibrated to Thermo Scientific Internal Reference Standard
Sufficient For: ELISA standard curve with replicates
1 vial MSDS for product #SMIL1B Mouse IL-1 beta Standard for ELISA $110.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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