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Mouse IFN gamma ELISA Kits


Microplate kits and reagent sets for sandwich ELISA of mouse interferon gamma (IFN-g).

Thermo Scientific Mouse IFN gamma ELISA Kits

Assay IFN-γ (mouse interferon gamma) levels in mouse serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples with our Thermo Scientific Mouse IFN gamma ELISA Kits.

These mouse IFN-gamma ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interferon gamma (IFNγ) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

  • Target: natural and recombinant mouse interferon gamma (IFNγ)
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 37 to 3000pg/mL
  • Sensitivity: < 10pg/mL
  • Precision: intra-assay CV < 10%; inter-assay CV < 10%
  • Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 4 hours
  • Sample Size: 50μL
  • Specificity: no significant cross-reaction with with mouse IL-1α, IL-1β, IL-3, IL-4, IL-6, IL-7, IL-10, GM-CSF, TNFα or human IFNα or IFNγ

Note: Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit.

Video of the Thermo Scientific IFN gamma ELISA Kit

Includes:

Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates.

» Compare ELISA Kit formats »

Details:

Mouse IFN gamma ELISA standard curve
Mouse IFNγ ELISA standard curve. An example standard curve for the Thermo Scientific Mouse IFNγ ELISA Kit (Part No. EM1001), with error bars based on five replicates for each interferon gamma standard concentration. Also see standard curve for the IFNγ Reagent Kit.

References:

Related Resources:

Related Products:

Looking for the matched antibody pair kit (Part No. KMIFNG)?
The original antibody pair kit associated with this ELISA kit has been discontinued. Consider using Cat. No. CMC4033, available at lifetechnologies.com.

Notice:

This product is for In Vitro experimental use only. Not for resale without express authorization.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
EM1001 Mouse IFN gamma ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-Mouse IFN gamma Precoated 96-well Strip Plate, 1 each
Lyophilized Recombinant Mouse IFN gamma Standards, 2 vials
Biotinylated Antibody Reagent, 8mL
Standard Diluent, 20mL
30X Wash Buffer, 50mL
Streptavidin-HRP Concentrate, 100μL
HRP Dilution Buffer, 14mL
TMB Substrate Solution, 13mL
Stop Solution, 13mL
Adhesive Plate Covers, 6 each
1-plate kit Product Instructions for product #EM1001 Mouse IFN gamma ELISA Kit MSDS for product #EM1001 Mouse IFN gamma ELISA Kit $399.00

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EM10012 Mouse IFN gamma ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 2 x 96 microplate wells

Kit Contents:
Anti-Mouse IFN gamma Precoated 96-well Strip Plate, 2 each
Lyophilized Recombinant Mouse IFN gamma Standards, 4 vials
Biotinylated Antibody Reagent, 2 x 8mL
Standard Diluent, 2 x 20mL
30X Wash Buffer, 2 x 50mL
Streptavidin-HRP Concentrate, 2 x 100μL
HRP Dilution Buffer, 2 x 14mL
TMB Substrate Solution, 2 x 13mL
Stop Solution, 2 x 13mL
Adhesive Plate Covers, 12 each
2-plate kit Product Instructions for product #EM10012 Mouse IFN gamma ELISA Kit MSDS for product #EM10012 Mouse IFN gamma ELISA Kit $650.00

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EM10015 Mouse IFN gamma ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plates
Sufficient For: 5 x 96 microplate wells

Kit Contents:
Anti-Mouse IFN gamma Precoated 96-well Strip Plate, 5 each
Lyophilized Recombinant Mouse IFN gamma Standards, 10 vials
Biotinylated Antibody Reagent, 5 x 8mL
Standard Diluent, 5 x 20mL
30X Wash Buffer, 5 x 50mL
Streptavidin-HRP Concentrate, 5 x 100┬ÁL
HRP Dilution Buffer, 5 x 14mL
TMB Substrate Solution, 5 x 13mL
Stop Solution, 5 x 13mL
Adhesive Plate Covers, 30 each
5-plate kit Product Instructions for product #EM10015 Mouse IFN gamma ELISA Kit MSDS for product #EM10015 Mouse IFN gamma ELISA Kit $1,500.00

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ESS0020 Mouse IFN gamma ELISA Reagent Kit
Formulation: Antibodies and complete reagent set for ELISA
Sufficient For: 5 x 96 microplate wells

Kit Contents:
IFN gamma Coating Antibody, 1 vial
IFN gamma Biotinylated Detection Antibody, 1 vial
Mouse IFN gamma Standard, 5 vials
Streptavidin-HRP Conjugate, 0.25mL
TMB Substrate Solution, 55mL
Stop Solution, 55mL
5-plate kit Product Instructions for product #ESS0020 Mouse IFN gamma ELISA Reagent Kit MSDS for product #ESS0020 Mouse IFN gamma ELISA Reagent Kit $375.00

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SM2IFNG Mouse IFN gamma Standard for ELISA
Formulation: Calibrated to NIBSC reference lot Gg-02-901-533
Sufficient For: ELISA standard curve with replicates
1 vial MSDS for product #SM2IFNG Mouse IFN gamma Standard for ELISA $110.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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