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Mouse IFN alpha ELISA Kit


Microplate sandwich ELISA kit for mouse interferon alpha (IFN-alpha).

VeriKine Mouse IFN alpha ELISA Kits

Assay IFN-α (mouse interferon alpha) levels in mouse cell and tissue culture media (supernatants) with this VeriKine* Mouse IFN alpha ELISA Kit (for Supernatant).

This mouse IFN alpha ELISA kit uses pre-titered, matched pairs of coating and detection antibodies to accurately measure interferon alpha (IFN-α) in samples at 100μL per well. The IFN-α kit is validated for use with mouse cell and tissue culture media. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kit contains pre-coated 96-well plate and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

  • Target: mouse interferon alpha (mouse IFN-α, IFN-alpha)
  • Format: colorimetric ELISA (TMB substrate) in clear 96-well plate
  • Measurement: absorbance at 450nm
  • Assay Range: 12.5 to 400pg/mL
  • Sensitivity: undetermined
  • Precision: intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
  • Sample Types: cell culture supernatant, tissue culture media
  • Total Assay Time: 27.25 hours (overnight)
  • Sample Size: 100μL
  • Specificity: no significant cross-reaction with mouse IFN-β, IFN-γ; human or rat IFN-α.
Video of the Thermo Scientific IFN alpha ELISA Kit

Includes:

Complete kit contains pre-coated antibody-plate, detection antibodies, buffers, diluents, standards, substrate, and stop solution.

Details:

Mouse IFN alpha ELISA standard curve
Mouse IFN-α ELISA standard curve. An example standard curve for the VeriKine* Mouse IFN-α ELISA Kit for Supernatants (Part No. 421201), with error bars based on three replicates for each interferon alpha standard concentration.

References:

Related Resources:

Related Products:

Notice:

This product is for In Vitro experimental use only. Not for resale without express authorization.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
421201 Mouse IFN alpha ELISA Kit (Supernatant)
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Precoated 96-well Plate, 1 each
Plate Sealers, 4 ea.
Wash Solution Concentrate, 50mL
Mouse IFN Alpha Standard 10,000pg/mL, 1 vial
Sample Buffer, 50mL
Antibody Concentrate, 1 vial
Concentrate Diluent, 15mL
HRP Conjugate Concentrate, 1 vial
Assay Diluent, 15mL
TMB Substrate Solution, 15mL
Stop Solution, 15mL
1-plate kit MSDS for product #421201 Mouse IFN alpha ELISA Kit (Supernatant) $599.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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