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Modified Lowry Protein Assay Kit


The accuracy of the Lowry method in a ready-to-use formulation.

Pierce Modified Lowry Protein Assay

The Thermo Scientific Pierce Modified Lowry Protein Assay is a stable form of a traditional, 2-component, folin phenol- and copper-based reagent system to measure (A750nm) total protein concentration compared to a protein standard.

The Modified Lowry Protein Assay Kit combines a stabilized formulation of the original Lowry Reagents and the essential Folin-Ciocalteu Phenol reagent in a complete kit for accurately determining protein concentration in a variety of samples types. Although newer protein assay methods provide greater speed and convenience, the Lowry method remains a popular, accurate and useful option for many applications.

Highlights:

  • Popular method – widely cited in protein research literature
  • Colorimetric – measure with a standard spectrophotometer or plate reader (750nm)
  • Stable – uses a modified cupric sulfate-tartrate reagent that is stable at room temperature
  • Large range – exhibits good linearity in the range 1 to 1500µg/mL (tested with BSA protein)
  • Convenient – microplate and cuvette protocols provided with the instructions

Product Details:

Modified Lowry Protein Assay Reagent protocol.

Protocol summary for the Thermo Scientific Modified Lowry Protein Assay.

 

Typcial standard curves for the Modified Lowry Protein Assay
Standard curves. Typical standard curves for bovine serum albumin (BSA) and bovine gamma globulin (BGG) in the Modified Lowry Protein Assay. Kit includes ampules of Albumin Standard.

References:

  1. Lowry, O.H., et al. (1951). J. Biol. Chem. 193, 265-275.
  2. Temel, R.E., et al. (2003). J. Biol. Chem. 278, 4792-4799.
  3. Nagai, Y., et al. (2000). J. Biol. Chem. 25, 10437-10442.

Protein Methods Library:

Overview of Protein Assays
Chemistry of Protein Assays
Protein Assay Data Analysis

Related Products:

BSA Assay StandardsBGG Assay Standards
Coomassie Plus, BCA, and Other Assay Reagents
Microplates for Protein Assays


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
23240 Modified Lowry Protein Assay Kit
Formulation: Multi-component kit
Sufficient For: 480 tube assays or 2400 microplate assays

Kit Contents:
Modified Lowry Protein Assay Reagent, 480mL
2N Folin-Ciocalteu Phenol Reagent, 50mL
Albumin Standard Ampules, 2mg/mL, 10 x 1mL
530mL kit Product Instructions for product #23240 Modified Lowry Protein Assay Kit MSDS for product #23240 Modified Lowry Protein Assay Kit Certificate of Analysis for product #23240 Modified Lowry Protein Assay Kit $146.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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