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MMTS (Methyl methanethiosulfonate)

Reversibly blocks cysteines and other sulfhydryl groups.

Pierce MMTS (methyl methanethiosulfonate)

Thermo Scientific Pierce MMTS (methyl methanethiosulfonate) is a small compound that reversibly blocks cysteines and other sulfhydryl groups, enabling the study of enzyme activation and other protein functions.

MMTS (methyl methanethiosulfonate) is a sulfhydryl-reactive compound that can reversibly sulfenylate thiol-containing molecules. Reacting MMTS with reduced sulfhydryls (-SH) results in their modification to dithiomethane (-S-S-CH3). Treatment with reducing agents, such as dithiothreitol (DTT), 2-mercaptoethanol (2-ME) or Tris(2-carboxyethyl) phosphine hydrochloride (TCEP), will cleave the disulfide groups to restore the original sulfhydryl. MMTS is commonly used to study biochemical pathways involving thiol-dependent enzymes.


  • Converts sulfhydryl groups on cysteine side chains into -S-S-CH3
  • Reaction is reversible with DTT or TCEP, restoring the free sulfhydryl
  • Used to modify thiol groups in creatine kinase

Product Details:

Methyl methanethiosulfonate; MMTS

Structure and properties of MMTS:

  • Chemical name: methyl methanethiosulfonate
  • Reactivity: sulfhydryl groups (adds methyl group via disulfide bond)
  • Chemical formula: C2H6O2S2
  • CAS number: 2949-92-0
  • Molecular weight: 126.20
  • Form: liquid
  • Boiling point: 85-87°C/0.8mm


More Product Data


  1. Kirley, T.L. (1989). Reduction and fluorescent labeling of cyst(e)ine-containing proteins for subsequent structural analyses. Anal Biochem 180:231.
  2. Smith, D.J., et al. (1975). Simple alkanethiol groups for temporary blocking of enzymes. Biochemistry 14:766.

Related Resources:

Overview of crosslinking and protein modification

Related Products:

S-nitrosylation Western Blot Kit (protocol uses MMTS)
Iodoacetamide – carboxymethylate sulfhydryls
N-ethylmaleimide – permanently block sulfhydryls
MM(PEG)n – methylate and pegylate sulfhydryls

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
23011 MMTS
Formulation: Methyl methanethiosulfonate; clear liquid
Sufficient For: 1585mL of typical 1X (1mM) reaction solution, or approx. 100 uses at 2mg reagent per use
200mg Product Instructions for product #23011 MMTS MSDS for product #23011 MMTS Certificate of Analysis for product #23011 MMTS $96.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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