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Mitochondria Isolation Kit for Tissue


Isolate mitochondrial proteins from soft and hard tissue.

Mitochondria Isolation Kit for Tissue

The Thermo Scientific Mitochondria Isolation Kit for Tissue enables isolation of intact mitochondria from soft and hard tissue samples in about an hour using reagent-based or Dounce homogenization methods.

This kit provides for two methods for mitochondria isolation. The first method uses a unique reagent-based procedure that enables simultaneous multi-sample processing. The second method relies on traditional Dounce homogenization for tissue disruption and subsequent isolation of the organelle. Both procedures use differential centrifugation to separate the intact mitochondria using a bench-top microcentrifuge and are completed in less than 60 minutes. In addition, both procedures have been optimized for maximum yield of mitochondria with minimal damage to integrity. The isolated mitochondria may be used for a number of downstream applications, including 1D and 2D Western blotting and protein profiling using mass spectroscopy.

Highlights:

  • Quick and Convenient – isolate intact mitochondria in less than 60 minutes
  • Versatile – offers two methods of isolation from both soft and hard tissue samples
  • Multi-sample format – reagent-based approach enables simultaneous processing of multiple samples
  • Optional alternate method – reagents and protocol included for the traditional Dounce homogenization procedure with fewer required strokes
  • Bench-top compatible – both procedures can be performed using a microcentrifuge tube

Product Details:

    COX4          VDAC      Cytochrome C
M C M C M C
Reagent-based Mitochondria isolation and protein extraction from tissue.
Dounce-based Mitochondria isolation by Dounce homogenization
Integrity of mitochondria extracted from tissues. Mitochondria (M) and cytosolic (C) fractions were prepared from fresh rat liver (Panels 1, 2, 3, 5 and 6) and heart (Panel 4) tissue samples using the reagent-based and Dounce homogenization methods of the Thermo Scientific Mitochondria Isolation Kit for Tissue. Fractions were analyzed via Western blot for COX4, VDAC and Cytochrome C mitochondrial proteins. Thermo Scientific SuperSignal West Pico Chemiluminescent Substrate (Part No. 34080) was used for detection. COX4 is an inner-mitochondria membrane protein, VDAC is an outer-mitochondria membrane protein and cytochrome C is located in the intermembrane space. Results indicate that cytosolic proteins were thoroughly separated from the isolated mitochondria.

References:

  1. Zhang, Q, et. al. (2010). Circulating Mitochondrial DAMPs Cause Inflammatory Responses to Injury. Nature. 464(7285):104-107.

Related Resources:

Review of cell fractionation and organelle isolation

Related Products:

Mitochondrial Isolation Kit for Cultured Cells
Protease and Phosphatase Inhibitors


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
89801 Mitochondria Isolation Kit for Tissue
Formulation: Multi-component kit
Sufficient For: 50 samples each containing 50 to 200mg of soft or hard tissue

Kit Contents:
Mitochondria Isolation Reagent A, 50mL
Mitochondria Isolation Reagent B, 0.5mL
Mitochondria Isolation Reagent C, 65mL
Bovine Serum Albumin, 235mg
PBS Pack (makes 500mL), 1 pack
115mL kit Product Instructions for product #89801 Mitochondria Isolation Kit for Tissue MSDS for product #89801 Mitochondria Isolation Kit for Tissue Certificate of Analysis for product #89801 Mitochondria Isolation Kit for Tissue $241.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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