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Pierce Microcentrifuge Spin Cups and Columns


Convenient tools to manipulate small volumes of affinity supports.

Pierce Spin Cups and Spin Columns

Thermo Scientific Pierce Spin Cups and Spin Columns are convenient tools for manipulating small volumes of affinity supports (5 to 500µL) for protein purification.

Simply add the affinity resin and sample to one of the affinity columns, then use a microcentrifuge to efficiently wash away contaminants and elute your purified sample without losing any resin in the process. Spin columns allow you to affinity purify more protein in less time.

Highlights (for IP and Co-IP):

  • Efficient washing of samples means fewer washes are needed to remove contaminating proteins
  • Efficient elution of samples means more antigen and co-precipitated proteins are recovered
  • No resin loss means more consistent IP and co-IP results
  • Decanting supernatant from IP or co-IP pellet is not necessary
  • Spin protocols drastically reduce the time required for IPs and co-IPs
  • Low protein binding polypropylene column construction minimizes nonspecific binding

Applications:

  • Affinity purification or affinity chromatography
  • Immunodepletion
  • Immunoprecipitation (IP)
  • Co-immunoprecipitation (co-IP)

Product Details:

Specifications of Pierce Spin Cups and Columns. All columns are made from low protein binding polypropylene for compatibility with protein purification, and fit into standard microcentrifuge tubes for use in most microcentrifuges.
Column Model Details
Paper filter spin cups for immunoprecipitation and affinity purification
View Dimensions of
Spin Cup – Paper Filter

Paper filters are resistant to clogging from cellular debris.
Column volume: 600µL
Resin volume: 20 to 300µL
Filter type: Paper filter, ~10µm pore size
Cap: Collection tube cap fits onto inserted spin cup

Cellulose acetate spin cups for immunoprecipitation and affinity purification
View Dimensions of
Spin Cup – Cellulose Acetate Filter
Smaller pore size than paper.

Column volume: 800µL
Resin volume: 20 to 400µL
Filter type: Cellulose acetate filter, 0.45µm pore size
Cap: Collection tube cap fits onto inserted spin cup
Screw cap spin columns for IP, co-IP and affinity separations
View Dimensions of
Spin Column with Screw Caps
Luer-Lok adaptors allow these columns to be used for syringe-based purifications.

Column volume: 900µL
Resin volume: 20 to 400µL
Filter type: Polyethylene filter, ~10µm pore size
Small & large frit options for different sample sizes
Cap: O-ring screw top caps
Press-in bottom plugs
Spin columns for IP, co-IP and affinity purification
View Dimensions of
Spin Column with Snap Caps
These are the spin columns used in the Cell Surface Protein Isolation and Glycoprotein Isolation Kits.

Column volume: 1000µL (1mL)
Resin volume: 20 to 500µL
Filter type / pore size: Polyethylene filter, ~30µm pore size
Cap Snap cap on column (no cap on collection tube)
Press-on bottom caps
Micro-Spin columns for IP, co-IP and affinity purification
View Dimensions of
Micro-Spin Column

For very small resin and sample volumes.

Column volume: 400µL
Resin volume: 5 to 100µL
Filter type: Polyethylene filter, ~30µm pore size
Cap: O-ring screw top caps
Press-on bottom caps

References:

  1. Anzai, N., et al. (2004). J. Biol. Chem. 279: 45942-50.
  2. Sokolov, M., et al. (2004). J. Biol. Chem. 279: 19149-56.
  3. Wu, X., et al. (2005). J. Biol. Chem. 280: 29964-70.
  4. Lee, J-S., et al. (2006) J. Virol. 80: 4079-87.

Related Resources:

Review of affinity purification methods
Review of immunoprecipitation

Related Products:

Larger spin columns for affinity purification
Gravity-flow columns for affinity purification
Affinity purification supports
Immunoprecipitation kits


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
69700 Pierce Spin Cups - Paper Filter
Formulation: Polypropylene (600µL capacity); Paper filter (10µm pore size); Collection tubes included
Sufficient For: Microcentrifuge procedures with 20 to 300µL resin
50 cups Certificate of Analysis for product #69700 Pierce Spin Cups - Paper Filter $118.00

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69702 Pierce Spin Cups - Cellulose Acetate Filter
Formulation: Polypropylene (800µL capacity); Cellulose-acetate filter (0.45µm pore size); Collection tubes included
Sufficient For: Microcentrifuge procedures with 20 to 400µL resin
50 cups $116.00

Add to cart
69705 Pierce Spin Columns - Screw Cap
Formulation: Polypropylene (900µL capacity); Polyethylene filter (10µm pore size); Screw caps with O-rings; Press-in bottom plugs
Sufficient For: Microcentrifuge procedures with 20 to 400µL resin
25 columns Product Instructions for product #69705 Pierce Spin Columns - Screw Cap Certificate of Analysis for product #69705 Pierce Spin Columns - Screw Cap $107.00

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69715 Pierce Microcentrifuge Tubes
Formulation: Polypropylene (1.5mL capacity); Attached snap-caps
Sufficient For: Sample collection with Part No. 69700
72 tubes $36.00

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69720 Pierce Microcentrifuge Tubes
Formulation: Polypropylene (2mL capacity); Attached snap-caps
Sufficient For: Sample collection with Part No. 69702
72 tubes $35.00

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69725 Pierce Spin Columns - Snap Cap
Formulation: Polypropylene (1mL capacity); Polyethylene filter (30µm pore size); Attached snap-cap; Press-on bottom plugs; 100 collection tubes included
Sufficient For: Microcentrifuge procedures with 20 to 500µL resin
50 columns $117.00

Add to cart
89879 Pierce Micro-Spin Columns
Formulation: Polypropylene (400µL capacity); Polyethylene filter (30µm pore size); Screw caps with O-rings; Press-in bottom plugs
Sufficient For: Microcentrifuge procedures with 5 to 100µL resin
50 columns Product Instructions for product #89879 Pierce Micro-Spin Columns Certificate of Analysis for product #89879 Pierce Micro-Spin Columns $59.00

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89879B Pierce Micro-Spin Columns
Centrifuge Columns, top caps and bottom caps
Custom Certificate of Analysis for product #89879B Pierce Micro-Spin Columns

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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