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Micro BCA Protein Assay Kit


Specially formulated for measuring dilute protein solutions.

Pierce Micro BCA Protein Assay Kit

The Thermo Scientific Pierce Micro BCA Protein Assay is a special 3-component version of our popular BCA Reagents, optimized to measure (A562nm) total protein concentration of dilute protein solutions (0.5 to 20 micrograms/mL).

The Micro BCA Protein Assay Kit is a specialized version of the popular Pierce BCA Protein Assay for determining the protein concentration of dilute samples. Mixing together the three Micro BCA Reagents results in a working solution that is sufficiently concentrated to measure protein when mixed with an equal volume of sample. The result is an assay for accurately measuring 0.5 to 20µg/mL protein solutions. The assay is exceptionally linear and exhibits very low levels of protein-to-protein variability.

Highlights:

  • Sensitive – accurately detect down to 0.5µg/mL (2µg/mL in microplate format)
  • High linearity – linear working range for BSA equals 0.5 to 20µg/mL
  • Colorimetric – measure with a standard spectrophotometer or plate reader (562nm)
  • Compatible – unaffected by typical concentrations of most ionic and nonionic detergents
  • Convenient – microplate and cuvette protocols provided with the instructions
  • Stable – kit stable at room temperature; prepared working reagent stable for to 24 hours

Product Details:

Video of the Pierce Micro BCA Protein Assay Kit in action!

 

Pierce Micro BCA Protein Assay Reagent protocol.
Protocol summary for the Thermo Scientific Pierce Micro BCA Protein Assay .

 

Typical standard curves for the Pierce Micro BCA Protein Assay
Standard curves. Typical standard curves for bovine serum albumin (BSA) and bovine gamma globulin (BGG) in the Micro BCA Protein Assay Kit. The kit includes ampules of Albumin Standard.

References:

  1. Smith, P.K., et al. (1985). Anal. Biochem. 150(1), 76-85.
  2. Kang, D.E., et al. (2002). Cell 110, 751-762.
  3. Rawadi, G., et al.. (1999). J. Immunol. 162, 2193-2203.
  4. Blum, D., et al. (2002). J. Neurosci. 22, 9122-9133.
  5. Paratcha, G., et al. (2003). Cell 113, 867-879.

Related Resources:

BCA Protein Assay Reagents FAQ
Protein Assay Selection Guide
Protein Assay Compatibility Table

Protein Methods Library:

Overview of Protein Assays
Chemistry of Protein Assays
Protein Assay Data Analysis

Compatible Instrumentation:

BioMate 3 UV-Vis Spectrophotometer
NanoDrop 2000c UV/Vis Spectrophotometer
NanoDrop 8000 UV/Vis 8 Sample Spectrophotometer

Related Products:

BSA Assay StandardsBGG Assay Standards
Coomassie Plus, BCA, and Other Assay Reagents
Microplates for Protein Assays


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
23235 Micro BCA Protein Assay Kit
Formulation: Multi-component kit
Sufficient For: 480 tube assays or 3200 microplate assays

Kit Contents:
Micro BCA Reagent A (MA), 240mL
Micro BCA Reagent B (MB), 240mL
Micro BCA Reagent C (MC), 12mL
Albumin Standard Ampules, 2mg/mL, 10 x 1mL
500mL kit Product Instructions for product #23235 Micro BCA Protein Assay Kit MSDS for product #23235 Micro BCA Protein Assay Kit Certificate of Analysis for product #23235 Micro BCA Protein Assay Kit $195.00

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23231 Micro BCA Reagent A (MA)
Formulation: Proprietary alkaline tartrate-carbonate buffer
Sufficient For: 3200 microplate assays when mixed with Reagents MB and MC
240mL MSDS for product #23231 Micro BCA Reagent A (MA) Certificate of Analysis for product #23231 Micro BCA Reagent A (MA) $53.00

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23232 Micro BCA Reagent B (MB)
Formulation: Proprietary bicinchonic acid solution
Sufficient For: 3200 microplate assays when mixed with Reagents MA and MC
240mL MSDS for product #23232 Micro BCA Reagent B (MB) Certificate of Analysis for product #23232 Micro BCA Reagent B (MB) $97.00

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23234 Micro BCA Reagent C (MC)
Formulation: Proprietary copper sulfate solution
Sufficient For: 3200 microplate assays when mixed with Reagents MA and MB
12mL MSDS for product #23234 Micro BCA Reagent C (MC) Certificate of Analysis for product #23234 Micro BCA Reagent C (MC) $32.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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