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MBS


An amine-to-sulfhydryl crosslinker with a short aromatic spacer arm (7.3 angstroms).

Pierce MBS

Thermo Scientific Pierce MBS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a short aromatic spacer arm (7.3 angstroms).

Highlights:

  • Reactive groups: NHS ester and maleimide
  • Reactive towards: amino and sulfhydryl groups
  • Non-cleavable
  • Water-insoluble (dissolve first in DMF or DMSO); compare to Sulfo-MBS
  • Popular for forming enzyme immunoconjugates

 

Product Details:

m-Maleimidobenzoyl-N-hydroxysuccinimide ester; MBS
 
Chemical structure of MBS crosslinking reagent.

References:

  1. Chrisey, L.A., et al. (1996). Nucleic Acids Res. 24(15), 3031-3039.
  2. Myers, D.E., Uckun, F.M., Swaim, S.E. and Vallera, D.A. (1989). The effects of aromatic and aliphatic maleimide crosslinkers and anti-CD-5 ricin immunotoxins. J. Immunol. Meth. 121, 129-142. (MBS example)
  3. Kitagawa, T. and Aikawa, T. (1976). Enzyme coupled immunoassay of insulin using a novel coupling reagent. J. Biochem. (Tokyo) 79, 233-236.

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Resources:

Overview of crosslinking
Chemistry of crosslinking

Related Products:

Crosslinkers at a glance (selection guide)


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
22311 MBS
Formulation: m-Maleimidobenzoyl-N-hydroxysuccinimide ester; crystalline powder solid
Sufficient For: 159mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use
50mg Product Instructions for product #22311 MBS MSDS for product #22311 MBS Certificate of Analysis for product #22311 MBS $74.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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