Update profile
    Change password
    Sign out


An amine-to-sulfhydryl crosslinker with a short aromatic spacer arm (7.3 angstroms).

Pierce MBS

Thermo Scientific Pierce MBS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a short aromatic spacer arm (7.3 angstroms).


  • Reactive groups: NHS ester and maleimide
  • Reactive towards: amino and sulfhydryl groups
  • Non-cleavable
  • Water-insoluble (dissolve first in DMF or DMSO); compare to Sulfo-MBS
  • Popular for forming enzyme immunoconjugates


Product Details:

m-Maleimidobenzoyl-N-hydroxysuccinimide ester; MBS
Chemical structure of MBS crosslinking reagent.


  1. Chrisey, L.A., et al. (1996). Nucleic Acids Res. 24(15), 3031-3039.
  2. Myers, D.E., Uckun, F.M., Swaim, S.E. and Vallera, D.A. (1989). The effects of aromatic and aliphatic maleimide crosslinkers and anti-CD-5 ricin immunotoxins. J. Immunol. Meth. 121, 129-142. (MBS example)
  3. Kitagawa, T. and Aikawa, T. (1976). Enzyme coupled immunoassay of insulin using a novel coupling reagent. J. Biochem. (Tokyo) 79, 233-236.

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Resources:

Overview of crosslinking
Chemistry of crosslinking

Related Products:

Crosslinkers at a glance (selection guide)

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
22311 MBS
Formulation: m-Maleimidobenzoyl-N-hydroxysuccinimide ester; crystalline powder solid
Sufficient For: 159mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use
50mg Product Instructions for product #22311 MBS MSDS for product #22311 MBS Certificate of Analysis for product #22311 MBS $74.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

Recommend this page

Follow Us
Email Sign-up  Email Announcements

You may also need
34080 - SuperSignal West Pico Chemiluminescent Substrate

32106 - Pierce ECL Western Blotting Substrate

22322 - Sulfo-SMCC

34075 - SuperSignal West Dura Extended Duration Substrate

34090 - CL-XPosure Film, 5 x 7in. (13 x 18cm)


Antibodies  |   Molecular Biology   |   Cell Biology   |  Thermo Scientific  |   * Trademarks  |   Privacy Statement
© 2014 Thermo Fisher Scientific Inc.   |   3747 N Meridian Rd, Rockford, IL USA 61101   |   1-800-874-3723  or  815-968-0747