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Amine-binding, Maleic Anhydride Activated Plates


Covalently binds proteins, peptides and other amine-containing compounds.

Pierce Maleic Anhydride Activated Plates

Thermo Scientific Pierce Maleic Anhydride Activated Plates allow attachment of amine-containing peptides and other molecules to microplate wells for use in binding assays.

These plates are useful for immobilizing peptides and other ligands that do not coat efficiently by passive adsorption. Reaction of the maleic anhydride groups with primary amines (-NH2) results in formation of amide bonds that are stable at neutral pH and above. Acidic conditions hydrolyze the bond; for example, the half-life of hydrolysis at pH 3.5 and 37°C is approximately 11 hours (Ref.1). Therefore, coating reactions are best performed at pH 8-9, and the reacted plates are best used at pH > 7 for ELISA and other methods.

Highlights:

  • Spontaneously react with primary amines
  • A direct, simple approach to covalently attach molecules to the well surface of an EIA plate
  • Maleic anhydride maintains its integrity and availability for coupling for months under dry, room temperature conditions

Product Details:

Reaction scheme for coupling both large and small amine-containing molecules to maleic anhydride coated microplates
Reaction scheme for coupling peptides and other amine-containing molecules.

References:

  1. Butler, P.J.G, et al. (1967). Reversible blocking of peptide amino groups by maleic anhydride. Biochem. J. 103, 78P
  2. Dumoutier, L., et al. (2001). J. Immunol. 166, 7090-7095.
  3. Marston, E.L., et al. (2002). Clin. Diagn. Lab. Immunol. 9, 446-452.
  4. Leu, S.-J., et al. (2003). J. Biol. Chem. 278, 33801-33808.
  5. Liao, Y.-F., et al. (2002). J. Biol. Chem. 277, 14467-14474.
  6. Batista, F.D. and Neuberger, M.S. (2000). EMBO J. 19, 513-520.

Related Resources:

Assay development and ELISA technical guide
Covalent protein immobilization technical guide

Related Products:

Maleimide Activated Plates (sulfhydryl-reactive)


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
15100 Maleic Anhydride Activated Plates, Clear, 8-Well Strip
Formulation: Clear polystyrene 96-well strip plates coated at 200μL/well with proprietary maleic anhydride-presenting molecular solution.
Sufficient For: Binding approx. 125pmol amine-molecule per well
5 plates Product Instructions for product #15100 Maleic Anhydride Activated Plates, Clear, 8-Well Strip MSDS for product #15100 Maleic Anhydride Activated Plates, Clear, 8-Well Strip Certificate of Analysis for product #15100 Maleic Anhydride Activated Plates, Clear, 8-Well Strip $156.00

Add to cart
15102 Maleic Anhydride Activated Plates, Clear, 8-Well Strip
Formulation: Clear polystyrene 96-well strip plates coated at 200μL/well with proprietary maleic anhydride-presenting molecular solution.
Sufficient For: Binding approx. 125pmol amine-molecule per well

5 x 5 plates Product Instructions for product #15102 Maleic Anhydride Activated Plates, Clear, 8-Well Strip MSDS for product #15102 Maleic Anhydride Activated Plates, Clear, 8-Well Strip $672.00

Add to cart
15108 Maleic Anhydride Activated Plates, White, 96-Well
Formulation: White polystyrene 96-well plates coated at 200μL/well with proprietary maleic anhydride-presenting molecular solution.
Sufficient For: Binding approx. 125pmol amine-molecule per well
5 plates Product Instructions for product #15108 Maleic Anhydride Activated Plates, White, 96-Well MSDS for product #15108 Maleic Anhydride Activated Plates, White, 96-Well Certificate of Analysis for product #15108 Maleic Anhydride Activated Plates, White, 96-Well $156.00

Add to cart
15110 Maleic Anhydride Activated Plates, Clear, 96-Well
Formulation: Clear polystyrene 96-well plates coated at 200μL/well with proprietary maleic anhydride-presenting molecular solution.
Sufficient For: Binding approx. 125pmol amine-molecule per well

5 plates Product Instructions for product #15110 Maleic Anhydride Activated Plates, Clear, 96-Well MSDS for product #15110 Maleic Anhydride Activated Plates, Clear, 96-Well Certificate of Analysis for product #15110 Maleic Anhydride Activated Plates, Clear, 96-Well $156.00

Add to cart
15112 Maleic Anhydride Activated Plates, Clear, 96-Well
Formulation: Clear polystyrene 96-well plates coated at 200μL/well with proprietary maleic anhydride-presenting molecular solution.
Sufficient For: Binding approx. 125pmol amine-molecule per well

5 x 5 plates Product Instructions for product #15112 Maleic Anhydride Activated Plates, Clear, 96-Well MSDS for product #15112 Maleic Anhydride Activated Plates, Clear, 96-Well $672.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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