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Pierce Magnetic Phosphopeptide Enrichment Kit


TiO2 magnetic particles for high throughput phosphopeptide isolation.

Thermo Scientific Pierce Magnetic Titanium Dioxide Phosphopeptide Enrichment Kit.

The Thermo Scientific Pierce Magnetic Titanium Dioxide Phosphopeptide Enrichment Kit is for isolating phosphopeptides from complex biological samples using titanium dioxide-coated magnetic beads.

The TiO2 ligand selectively binds peptides containing phosphorylated serine (Ser), tyrosine (Tyr) or threonine (Thr), enabling phosphopeptide enrichment from protease-digested samples. The isolated phosphopeptides are compatible for analysis downstream by mass spectrometry. The high-performance, iron oxide, superparamagnetic particles are validated and optimized for use with high-throughput magnetic platforms, such as the Thermo Scientific KingFisher 96 and KingFisher Flex Instruments. The beads also enable premium performance for simple benchtop applications using an appropriate magnetic stand.

Highlights:

  • Complete MS-compatible Kits – include ready-to-use binding, wash and elution buffers that are optimized for phosphopeptide enrichment and downstream analysis by MALDI and ESI mass spectrometry
  • Optimized for HTS – procedure validated for processing 1 to 96 samples at a time; complete entire assay in about 15 minutes using a KingFisher Flex Instrument
  • Stable affinity ligand – titanium dioxide is specially coated as a film on the magnetic particles
  • Selective – affinity system is selective for phosphorylated Ser, Tyr and Thr; exhibits minimal non-specific binding to acidic residues
  • Sensitive – affinity provides more than 1000 times greater sensitivity than traditional IMAC technologies; enables enrichment and MS-measurement of less than 100 fmol of phosphoprotein

Product Details:

Phosphopeptide enrichment improves MS-identification of phosphoproteins. Two micrograms of a tryptic digest prepared from periferal blood mononuclear cells (lymphocytes) with and without phosphopeptide enrichment were analyzed by MS. Enrichment was performed with the Thermo Scientific Pierce Titanium Dioxide Phosphopeptide Enrichment Kit using the Thermo Scientific KingFisher 96 Instrument. Samples were analyzed on an Thermo Scientific LTQ Orbitrap Mass Spectrometer.
Measurement Parameter Enriched Non-Enriched
Total number of proteins identified 185 247
Total number of phosphoproteins identified 160 1
Total number of peptides identified 2347 2457
Total number of phosphopeptides identified 2009 7
Total number of unique phosphopeptides identified 177 1
Relative enrichment for phosphopeptides (%) 86 0.3

More Product Data

Related Resources:

Review of automated magnetic separations for proteomics (includes selection guide)
Review of sample preparation for mass spectrometry

Related Products:

Other Pierce Magnetic Beads
MagnaBind Magnetic Beads for manual applications
Thermo Scientific KingFisher Magnetic Particle Processors


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
88811 Pierce Magnetic Titanium Dioxide Phosphopeptide Enrichment Kit
Formulation: Multi-component kit
Sufficient For: Purifying 96 x 100µg peptide samples

Kit Contents:
TiO2 Magnetic Beads (20X), 1mL
Binding Buffer, 100mL
Washing Buffer, 25mL
Elution Buffer, 3mL
Thermo-Fast 96 Robotic PCR Plates, 0.2mL wells, 2 plates
96-rxn kit Product Instructions for product #88811 Pierce Magnetic Titanium Dioxide Phosphopeptide Enrichment Kit MSDS for product #88811 Pierce Magnetic Titanium Dioxide Phosphopeptide Enrichment Kit Certificate of Analysis for product #88811 Pierce Magnetic Titanium Dioxide Phosphopeptide Enrichment Kit $520.00

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88812 Pierce Magnetic Titanium Dioxide Phosphopeptide Enrichment Kit, Trial Size
Formulation: Multi-component kit
Sufficient For: Purifying 24 x 100µg peptide samples

Kit Contents:
TiO2 Magnetic Beads (20X), 0.25mL
Binding Buffer, 100mL
Washing Buffer, 25mL
Elution Buffer, 3mL
Thermo-Fast 96 Robotic PCR Plates, 0.2mL wells, 2 plates
24-rxn kit Product Instructions for product #88812 Pierce Magnetic Titanium Dioxide Phosphopeptide Enrichment Kit, Trial Size MSDS for product #88812 Pierce Magnetic Titanium Dioxide Phosphopeptide Enrichment Kit, Trial Size Certificate of Analysis for product #88812 Pierce Magnetic Titanium Dioxide Phosphopeptide Enrichment Kit, Trial Size $320.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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