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MagnaBind Beads and Magnets


Activated with different ligands for use in small-scale benchtop magnetic affinity protocols.

MagnaBind Magnetic Beads and Supports

Thermo Scientific MagnaBind Magnetic Beads are a convenient affinity support for a variety of assays, allowing easy benchtop purification without columns or centrifugation.

MagnaBind Magnetic Beads are available with streptavidin, Protein A, Protein G, GAM IgG, GAR IgG and other ligands for use in small-scale benchtop magnetic affinity protocols. MagnaBind Beads that are pre-coated with Protein A, Protein G, streptavidin, anti-mouse or anti-rabbit antibodies are ready to use for purification of respective affinity targets. Activated beads, with either amine or carboxyl groups, are enable covalent attachment of antibodies or other ligands for customized affinity separation procedures.

Highlights:

  • Available pre-coated with streptavidin, secondary antibodies, Protein A or G
  • Available derivatized with carboxyl or amine groups for covalent attachment of ligands
  • Respond well to magnetic fields but have no magnetic memory
  • Most separations require a short 5- to 10-minute bench-top procedure
  • Bead specifications – surface area: >100 m2/g, 1 x 108 particles/mg, 1 to 4µm diameter
  • Can be used for either positive or negative selection
  • Cells can be isolated via direct or indirect capture of primary antibodies to cell surface antigens
  • Simple sterilization

Applications:

  • Cell sorting using either positive or negative selection
  • Protein purification or immunoassays using either direct or indirect methods

References:

  1. Nagano, S., et al. (2004). J. Biol. Chem. 279, 14673-14678.
  2. Deaton, J., et al. (2004). Protein Sci. 13, 1778-1786.
  3. Dutta, S., et al. (2003). Proc. Nat. Acad. Sci. USA 100, 12295-12300.
  4. Neaey, S.E., et al. (2001). J. Biol. Chem. 276(9), 6645-6655.
  5. Chaudhuri, T.K., et al. (2001). Cell 107, 235-246.
  6. Ilver, D., et al. (1998). Science 279, 373-377.

Related Resources:

Review of magnetic separations for proteomics (includes selection guide)

Related Products:

Pierce Magnetic BeadsStreptavidinProtein AG, A, G, LNi-NTAGlutathioneAnti-HA


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
21344 MagnaBind Streptavidin Beads
Formulation: Streptavidin on silanized iron oxide particles (1 to 4µm dia.) in buffer slurry
Sufficient For: Binding approx. 2µg (8nmol) of biotin per mL of beads
5mL Product Instructions for product #21344 MagnaBind Streptavidin Beads MSDS for product #21344 MagnaBind Streptavidin Beads Certificate of Analysis for product #21344 MagnaBind Streptavidin Beads $318.00

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21348 MagnaBind Protein A Beads
Formulation: Protein A on silanized iron oxide particles (1 to 4µm dia.) in buffer slurry
Sufficient For: Binding approx. 0.2mg of rabbit IgG per mL of beads
5mL Product Instructions for product #21348 MagnaBind Protein A Beads MSDS for product #21348 MagnaBind Protein A Beads Certificate of Analysis for product #21348 MagnaBind Protein A Beads $417.00

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21349 MagnaBind Protein G Beads
Formulation: Protein G on silanized iron oxide particles (1 to 4µm dia.) in buffer slurry
Sufficient For: Binding approx. 0.2mg of mouse IgG per mL of beads
5mL Product Instructions for product #21349 MagnaBind Protein G Beads MSDS for product #21349 MagnaBind Protein G Beads Certificate of Analysis for product #21349 MagnaBind Protein G Beads $417.00

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21349B MagnaBind Protein G Beads
Formulation: Protein G on silanized iron oxide particles (1 to 4µm dia.) in buffer slurry
Sufficient For: Binding approx. 0.2mg of mouse IgG per mL of beads
Custom MSDS for product #21349B MagnaBind Protein G Beads Certificate of Analysis for product #21349B MagnaBind Protein G Beads
21352 MagnaBind Amine Derivatized Beads
Formulation: Amine-activated silanized iron oxide particles (1 to 4µm dia.) in buffer slurry
Sufficient For: Immobilizing ligand to beads having approx. 12µmol of amines per mL
5mL Product Instructions for product #21352 MagnaBind Amine Derivatized Beads MSDS for product #21352 MagnaBind Amine Derivatized Beads Certificate of Analysis for product #21352 MagnaBind Amine Derivatized Beads $139.00

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21353 MagnaBind Carboxyl Derivatized Beads
Formulation: Carboxyl-activated silanized iron oxide particles (1 to 4µm dia.) in buffer slurry
Sufficient For: Immobilizing ligand to beads having approx. 4.8µmol of carboxyls per mL
5mL Product Instructions for product #21353 MagnaBind Carboxyl Derivatized Beads MSDS for product #21353 MagnaBind Carboxyl Derivatized Beads Certificate of Analysis for product #21353 MagnaBind Carboxyl Derivatized Beads $137.00

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21354 MagnaBind Goat Anti-Mouse IgG
Formulation: Antibody on silanized iron oxide particles (1 to 4µm dia.) in buffer slurry
Sufficient For: Binding approx. 0.2mg of mouse IgG per mL of beads
50mL Product Instructions for product #21354 MagnaBind Goat Anti-Mouse IgG MSDS for product #21354 MagnaBind Goat Anti-Mouse IgG Certificate of Analysis for product #21354 MagnaBind Goat Anti-Mouse IgG $295.00

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21356 MagnaBind Goat Anti-Rabbit IgG
Formulation: Antibody on silanized iron oxide particles (1 to 4µm dia.) in buffer slurry
Sufficient For: Binding approx. 0.2mg of rabbit IgG per mL of beads
50mL Product Instructions for product #21356 MagnaBind Goat Anti-Rabbit IgG MSDS for product #21356 MagnaBind Goat Anti-Rabbit IgG Certificate of Analysis for product #21356 MagnaBind Goat Anti-Rabbit IgG $295.00

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21357 MagnaBind Magnet for 1.5mL Microcentrifuge Tube
Formulation: Plastic holder containing strong, rare-earth magnet
Sufficient For: Collecting magnetic particles in 1 microcentrifuge tube at a time
1 magnet $164.00

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21358 MagnaBind Magnet for 96-Well Plate Separator
Formulation: Plastic holder containing strong, rare-earth magnet
Sufficient For: Collecting magnetic particles in wells of standard 96-well microplates
1 magnet $450.00

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21359 MagnaBind Magnet for 6 Microcentrifuge Tubes
Formulation: Plastic holder containing strong, rare-earth magnet
Sufficient For: Collecting magnetic particles in 6 microcentrifuge tube at a time
1 magnet $328.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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