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LDS Sample Buffer, Non-Reducing (4X)


An alternative nonreducing sample buffer for various commercial gels.

Pierce LDS Sample Loading Buffer (4X)

Thermo Scientific Pierce LDS Sample Loading Buffer (4X) is a nonreducing lithium dodecyl sulfate sample loading buffer, a unique alternative to homemade and other commercial gel-loading dyes.

LDS Sample Buffer, Non-Reducing (4X) is a convenient sample buffer for use in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). The buffer contains coomassie dye, enabling visualization of the electrophoretic progress by the location of the dye front. The LDS Sample Buffer, Non-Reducing (4X) may be used in denaturing gels and is compatible with both coomassie and silver staining and Western blotting applications.

Highlights:

  • Concentrated – 4X formulation provides more versatility than traditional 2X buffers
  • Nonreducing – ready to use for non-reducing SDS-PAGE, or the preferred type and amount of reducing agent (e.g., DTT) can be added to produce reducing conditions
  • SDS alternative – lithium dodecyl sulfate (LDS) replaces sodium dodecyl sulfate (SDS) of traditional loading buffers but functions equally well
  • Physiologic pH – buffered with triethanolamine (pH 7.6) rather than Tris (pH 6.8)
  • Lane marker dyes – includes trace amounts of phenol red and coomassie G250 as visible markers of the dye front
  • Convenient – stable at room temperature, enabling storage at the bench where electrophoresis is performed

Product Details:

Formulation of the Thermo Scientific Pierce LDS Sample Loading Buffer (4X).

40% glycerol
4% lithium dodecyl sulfate (LDS)
4% Ficoll™ 400
0.8 M triethanolamine-Cl pH 7.6
0.025% phenol red
0.025% coomassie G250
2mM EDTA disodium

Related Resources:

Protein Molecular Weight Markers – product comparison and selection guide
Protein Gel Stains and Kits – compare colorimetric and fluorescent gel stains

Related Products:

Precise Protein Gels – browse all pre-cast gels for SDS-PAGE
BupH Dry Blend Electrophoresis Buffers – Tris-HEPES-SDS and other pouched electrophoresis buffers
Reducing Agents – 2-mercaptoethanol, DTT, TCEP and other reducing agents
Western blotting substrates and reagents – find kits, stripping buffers, blotting membranes and film


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
84788 LDS Sample Buffer, Non-Reducing (4X)
Formulation: 40% glycerol, 4% lithium dodecyl sulfate (LDS), 4% Ficoll*-400, 0.8M triethanolamine-Cl pH 7.6, 0.025% phenol red, 0.025% coomassie G250, 2mM EDTA disodium
Sufficient For: 400 to 2000 samples (20mL total) for SDS-PAGE
5mL Product Instructions for product #84788 LDS Sample Buffer, Non-Reducing (4X) MSDS for product #84788 LDS Sample Buffer, Non-Reducing (4X) $36.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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