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KMUH


A long crosslinker for conjugating glycoprotein carbohydrates to sulfhydryl groups.

Pierce KMUH

Thermo Scientific Pierce KMUH is a long, maleimide-and-hydrazide crosslinker for conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).

Highlights:

  • Reactive groups: maleimide and hydrazide
  • Reactive towards: sulfhydryl groups and carbonyl (aldehyde) groups
  • Long (19.0A), sulfhydryl-to-aldehyde crosslinker with simple aliphatic spacer arm (noncleavable)
  • Maleimide group reacts with sulfhydryl groups to form stable thioether linkages
  • Hydrazide group conjugates to oxidized sugars of glycoproteins and carbohydrates
  • Use sodium meta-periodate to oxidize glycosylation (e.g., sialic acid) to reactive aldehyde groups
  • Use with EDC to conjugate primary amine of hydrazide group to carboxyl groups

Product Details:

N-kappa-Maleimidoundecanoic acid hydrazide-TFA; KMUH
 
Chemical structure of KMUH crosslinking reagent.

References:

  1. Trail, P.A., et al. (1993). Cure of xenografted human carcinomas by BR96-doxorubicin immunoconjugates. Science 261, 212-215.

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Resources:

Overview of crosslinking
Chemistry of crosslinking
Protein glycosylation technical guide

Related Products:

Crosslinkers at a glance (selection guide)


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
22111 KMUH
Formulation: N-kappa-Maleimidoundecanoic acid hydrazide-TFA; solid
Sufficient For: 122mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use
50mg Product Instructions for product #22111 KMUH MSDS for product #22111 KMUH Certificate of Analysis for product #22111 KMUH $251.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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