Thermo Scientific Pierce KMUH is a long, maleimide-and-hydrazide crosslinker for conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).
- Reactive groups: maleimide and hydrazide
- Reactive towards: sulfhydryl groups and carbonyl (aldehyde) groups
- Long (19.0A), sulfhydryl-to-aldehyde crosslinker with simple aliphatic spacer arm (noncleavable)
- Maleimide group reacts with sulfhydryl groups to form stable thioether linkages
- Hydrazide group conjugates to oxidized sugars of glycoproteins and carbohydrates
- Use sodium meta-periodate to oxidize glycosylation (e.g., sialic acid) to reactive aldehyde groups
- Use with EDC to conjugate primary amine of hydrazide group to carboxyl groups
|Chemical structure of KMUH crosslinking reagent.
- Trail, P.A., et al. (1993). Cure of xenografted human carcinomas by BR96-doxorubicin immunoconjugates. Science 261, 212-215.
Crosslinker Application Guide -- search for recent literature references for this product
Overview of crosslinking
Chemistry of crosslinking
Protein glycosylation technical guide
Crosslinkers at a glance (selection guide)