Thermo Scientific Imperial Protein Stain is a ready-to-use colorimetric stain formulated with coomassie dye R-250 that delivers consistent nanogram-level detection of proteins in polyacrylamide electrophoresis gels.
Imperial Protein Stain is a coomassie dye reagent for detection of protein bands in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and 2D gels. The stain is a unique formulation of coomassie brilliant blue R-250 that delivers substantial improvements in protein-staining performance compared to homemade or other commercial stains. Staining results in intensely colored protein bands that are easy to photograph and document with gel imagers. This reagent is one of the most sensitive colorimetric stains available, easily detecting 3 to 6 nanograms of protein per band. The Imperial Protein Stain protocol uses simple water-wash steps rather than methanol/acetic acid fixation and destaining, which saves valuable preparation time and minimizes reagent costs.
- Sensitive – detects less than 3ng protein per band with the enhanced protocol (3 hours)
- Fast – ready-to-use reagent detects less than 6ng protein per band in just 20 minutes
- Robust – highly consistent, reproducible protein staining technique
- High contrast – intense purple bands are easier to photograph or scan than typical coomassie blue stains
- Versatile – compatible with downstream mass spectrometry analysis and protein sequencing
- Convenient – water washes only; no acid-fixative or methanol destaining solutions required
- Stable – 1-year room-temperature stability ensures consistent performance and saves refrigerator space
- Flexible – adjust staining and washing times to meet time- and sensitivity-requirements
|Protocol summary for the Thermo Scientific Imperial Protein Stain.
Imperial Protein stain is a unique formulation of Coomassie Brilliant Blue R-250 that delivers substantial improvements in protein-staining performance compared to homemade or other commercially available coomassie-based stains. The easy-to-follow protocol is flexible to meet demanding time and sensitivity requirements. The use of Imperial Protein Stain does not result in the problems (e.g., staining consistency) that can be associated with other coomassie G-250 stain preparations. In addition to faster protein band development and improved sensitivity over standard coomassie G-250 stains, Imperial Protein Stain does not require methanol/acetic acid fixation and destaining, saving valuable preparation time and minimizing reagent cost.
|Enhanced sensitivity and crystal-clear background using Imperial Protein Stain. For even greater sensitivity and reduced background, gels can be stained with Imperial Protein Stain for 1 hour and washed with water from 1 hour to overnight. Lane 1: BSA only (6µg), Lane 2-9: Loaded left to right at 1000ng, 200ng, 100ng, 50ng, 25ng, 12ng, 6ng and 3ng, respectively.
|Thermo Scientific Imperial Protein Stain is fast and sensitive. Proteins were separated on 4-20% Tris-glycine gels, stained for 5 minutes and destained 3 x 5 minutes in water. Lane 1. BSA only (6µg), Lanes 2-9. Indicated proteins loaded left to right at 1000ng, 200ng, 100ng, 50ng, 25ng, 12ng, 6ng and 3ng, respectively.
Protein Gel Stains Selection Guide
Protein gel electrophoresis technical guide
Process stained polyacrylamide gel pieces for mass spectrometry (Tech Tip #50)
Purify proteins from polyacrylamide gels (Tech Tip #51)
All Protein Gel Stains (coomassie, silver, fluorescent, etc.)
Reversible Protein Stains for PVDF and Nitrocellulose membranes