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Pierce Iminobiotin Agarose


Purify or remove streptavidin or avidin protein components.

Pierce Iminobiotin AgaroseThermo Scientific Pierce Iminobiotin Agarose provides for unusual affinity purification experiments involving clean-up or removal of streptavidin or avidin protein components.

Pierce Iminobiotin Agarose is beaded agarose support onto which iminobiotin moieties have covalently immobilized. Typical avidin-biotin affinity procedures use immobilized avidin or streptavidin proteins to capture biotin-tagged molecules from a sample. Occasionally, however, the reverse situation arises in which immobilized biotin in needed to capture avidin or streptavidin components from a sample. This iminobiotin resin addresses this special situation.

Highlights:

  • Enable purification or removal of avidin or streptavidin samples
  • Crosslinked beaded agarose onto which iminobiotin is covalently immobilized
  • Use Iminobiotin Agarose for reversible binding (e.g., non-denaturing purification of avidin)

Product Details:

Immobilized iminobiotin is the guanido analog of biotin. The dissociation constant of the avidin-iminobiotin complex is pH dependent. At pH 9.5 to 11, the avidin-iminobiotin complex will bind tightly. At pH 4, the avidin-iminobiotin complex will dissociate. Because no denaturing agents such as 8M guanidine hydrochloride or 4M urea are required for elution, the avidin conjugate has a better chance of maintaining its activity after purification. Thus, iminobiotin agarose can be used for situations requiring reversible binding and elution of avidin components.

Chemical structure of iminobiotin agarose.
 
Chemical structure of Thermo Scientific Pierce Iminobiotin Agarose. Each porous agarose bead is 45 to 165µm diameter and contains trillions of iminobiotin groups.

References (for both biotin and iminobiotin):

  1. Chaiet, I. and Wolf, F.J. (1964). Arch. Biochem. Biophys. 106, 1-5.
  2. Green, N.M. (1975). Avidin. Advances in Protein Chemistry. New York: Academic Press, pp. 29, 85-133.
  3. Hofmann, K., Wood, S.W., Brinton, C.C., Montibeller, J.A. and Finn, F.M. (1980). Proc. Natl. Acad. Sci. 77(8), 4666-4668.
  4. Wood, G.S. and Warnke, R. (1981). J. Histochem. Cytochem. 29, 1196-1204.
  5. Gitlin, G., Bayer, E.A. and Wilchek, M. (1987). Biochem. J. 242, 923-926.
  6. Hiller, Y., Gershoni, J.M., Bayer, E.A. and Wilchek, M. (1987). Biochem. J. 248, 167-171.
  7. Alon, R., Bayer, E.A. and Wilchek, M. (1990). Biochem. Biophys. Res. Comm. 170, 1236-1241.
  8. Gao, C., et al. (1997) Proc. Natl. Acad. Sci. 94, 11777-11782.
  9. Pazy, Y., et al. (2003). J. Biol. Chem. 278(9), 7131-7134.

Related Resources:

Review of affinity purification
Review of avidin-biotin interaction

Related Products:

Biotin Agarose (for irreversible binding)


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
20221 Pierce Iminobiotin Agarose
Formulation: Iminobiotin on crosslinked 6% beaded agarose, slurried in water with sodium azide
Sufficient For: Binding ≥ 1mg of avidin per mL of resin
5mL Product Instructions for product #20221 Pierce Iminobiotin Agarose MSDS for product #20221 Pierce Iminobiotin Agarose Certificate of Analysis for product #20221 Pierce Iminobiotin Agarose $124.00

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20221B Pierce Iminobiotin Agarose
Formulation: Iminobiotin on crosslinked 6% beaded agarose, slurried in water with sodium azide
Sufficient For: Binding = 1mg of avidin per mL of resin
Custom MSDS for product #20221B Pierce Iminobiotin Agarose Certificate of Analysis for product #20221B Pierce Iminobiotin Agarose

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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