Thermo Scientific Pierce Hydroxylamine-HCl has many uses, which includes cleaving Asn-Gly peptide bonds and deprotecting (exposing) sulfhydryls on SATA-modified molecules for conjugation.
Hydroxylamine•HCl is a strong reducing agent that is useful in biochemical crosslinking applications, including the deacetylation of SATA and chemical cleavage of EGS and Sulfo-EGS. Hydroxylamine converts carbonyl compounds (aldehydes and ketones) to their oxime derivatives in the presence of a weak base. Therefore, crosslinkers and other compounds that contain a carbonyl within their structure are cleavable with hydroxylamine•HCl.
- Quench amine-labeling or crosslinking reactions (e.g., with NHS esters)
- Expose protected sulfhydryl groups on SATA-modified molecules
- Cleave carbonyl-containing crosslinkers such as EGS and Sulfo-EGS
Structure and properties of hydroxylamine:
- Chemical name: hydroxylamine hydrochloride
- Chemical formula: NH2OH•HCl
- Molecular weight: 69.49
- CAS number: 5470-11-1
- Form: white crystals, free of foreign material
- Solubility: 10% (w/v)
- Abdella, P., et al. (1979). A new cleavable reagent for cross-linking and reversible immobilization of proteins. Biochem Biophys Res Commun 87(3):732-42.
- Duncan, R. J. S., et al. (1983). A new reagent which may be used to introduce sulfhydryl groups into proteins, and its use in the preparation of conjugates for immunoassay. Anal Biochem 132:68-73.
Overview of crosslinking and protein modification