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Hydroxylamine-HCl


Exposes sulfhydryls of SATA-modified proteins and cleaves Asn-Gly bonds.

Pierce Hydroxylamine-HCl

Thermo Scientific Pierce Hydroxylamine-HCl has many uses, which includes cleaving Asn-Gly peptide bonds and deprotecting (exposing) sulfhydryls on SATA-modified molecules for conjugation.

Hydroxylamine•HCl is a strong reducing agent that is useful in biochemical crosslinking applications, including the deacetylation of SATA and chemical cleavage of EGS and Sulfo-EGS. Hydroxylamine converts carbonyl compounds (aldehydes and ketones) to their oxime derivatives in the presence of a weak base. Therefore, crosslinkers and other compounds that contain a carbonyl within their structure are cleavable with hydroxylamine•HCl.

Highlights:

  • Quench amine-labeling or crosslinking reactions (e.g., with NHS esters)
  • Expose protected sulfhydryl groups on SATA-modified molecules
  • Cleave carbonyl-containing crosslinkers such as EGS and Sulfo-EGS

Product Details:

Hydroxylamine Hydrochloride

Structure and properties of hydroxylamine:

  • Chemical name: hydroxylamine hydrochloride
  • Chemical formula: NH2OH•HCl
  • Molecular weight: 69.49
  • CAS number: 5470-11-1
  • Form: white crystals, free of foreign material
  • Solubility: 10% (w/v)

References:

  1. Abdella, P., et al. (1979). A new cleavable reagent for cross-linking and reversible immobilization of proteins. Biochem Biophys Res Commun 87(3):732-42.
  2. Duncan, R. J. S., et al. (1983). A new reagent which may be used to introduce sulfhydryl groups into proteins, and its use in the preparation of conjugates for immunoassay. Anal Biochem 132:68-73.

Related Resources:

Overview of crosslinking and protein modification


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
26103 Hydroxylamine-HCl
Formulation: Hydroxylamine hydrochloride; crystalline solid
Sufficient For: 720mL of typical 1X (500mM) reaction solution
25g Product Instructions for product #26103 Hydroxylamine-HCl MSDS for product #26103 Hydroxylamine-HCl Certificate of Analysis for product #26103 Hydroxylamine-HCl $44.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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