Update profile
    Change password
    Sign out

One website. 70,000+ products. Your one-stop destination for essentials, documentation, and research resources.
piercenet.com becomes part of lifetechnologies.com on January 26, 2015  Find out more >

Hydrochloric Acid Solution

6N HCl in convenient, ready-to-use ampules for amino acid analysis.

Pierce Hydrochloric Acid Solution

Thermo Scientific Pierce Hydrochloric Acid Solution is a constant-boiling 6N HCl solution, supplied in 1mL ampules, that is specifically formulated as a sequencing-grade reagent for total protein hydrolysis for amino acid analysis.

A variety of products are available for peptide hydrolysis, amino acid analysis and HPLC separation. This constant boiling HCl is specially purified to effect peptide hydrolysis at 150°C, to ensure the integrity of your amino acid analysis data and to maximize sensitivity by eliminating interferences in your amino acid analysis. Pierce Hydrochloric Acid is purified and packaged to ensure a ninhydrin negative blank on hydrolysis. Convenient, pre-scored ampule packaging of the ready-to-use HCl maintains reagent integrity. This virtually eliminates exposure to laboratory atmospheres, fingerprints and other contaminants resulting from pipetting from bulk bottles.


  • Hydrolyzes peptides in 6 hours at 150°C
  • Specially purified to give a ninhydrin-negative blank on hydrolysis
  • Packaged in ampules to eliminate contamination and ensure product integrity

Product Details:

Eveleigh and Winter (1970) give an excellent description of the total protein hydrolysis technique using Constant Boiling Hydrochloric Acid. Standard protein hydrolysis conditions are 105-110°C for 16-24 hours. At 150°C, this reagent can hydrolyze peptides in 6 hours.

Chemical structure of hydrochloric acid, HCl Chemical structure of hydrochloric acid (HCl).


  1. Eveleigh, J.W. and Winter, G.D. (1970). Protein Sequence Determination, Ed Needleman, S.B., Springer-Verlag, pp 92-95.
  2. Blankenship, et al. (1989). High-sensitivity amino acid analysis by derivatization with o-Phthalaldehyde and 9-Fluorescence detection: applications in protein structure determination. Anal. Biochem. 178, 227-232.
  3. HurIey, J.B., et al. (1984). Isolation and characterization of a cDNA clone for the λ subunit of bovine retinal transducin. Proc. Natl. Acad. Sci. U.S.A. 81, 6948-6952.
  4. Lee, K., et al. (1979). Derivatization of cysteine and cystine for fluorescence amino acid analysis with the o-Phthaldialdehyde/ 2-mercaptoethanol reagent. J. Biol. Chem. July 25, 6248-6251.

Related Products:

Vacuum Hydrolysis Tubes
Amino Acid Standard H
Amino Acid Detection Reagents
Solvents for HPLC

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
24308 Hydrochloric Acid Solution, Sequencing Grade
Formulation: 6.1±0.2N hydrochloric acid in water
Sufficient For: Use neet (undiluted) in vacuum hydrolysis methods
10 x 1mL MSDS for product #24308 Hydrochloric Acid Solution, Sequencing Grade Certificate of Analysis for product #24308 Hydrochloric Acid Solution, Sequencing Grade $46.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

Recommend this page

Follow Us
Email Sign-up  Email Announcements

You may also need
21059 - Restore Western Blot Stripping Buffer

88323 - Pierce LTQ Velos ESI Positive Ion Calibration Solution

24590 - GelCode Blue Stain Reagent

46430 - Restore PLUS Western Blot Stripping Buffer

32134 - Pierce ECL Plus Western Blotting Substrate


Antibodies  |   Molecular Biology   |   Cell Biology   |  Thermo Scientific  |   * Trademarks  |   Privacy Statement
© 2015 Thermo Fisher Scientific Inc.   |   3747 N Meridian Rd, Rockford, IL USA 61101   |   1-800-874-3723  or  815-968-0747